{"product_id":"recombinant-human-pdgf-bb","title":"Recombinant Human PDGF-BB","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:10ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDescription\u003c\/b\u003e:Recombinant Human Platelet-Derived Growth Factor BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e:P01127\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:12.42 KDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApparent molecular weight\u003c\/b\u003e:14 KDa, reducing conditions\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOther names\u003c\/b\u003e:PDGFBB; PDGF-BB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage condition\u003c\/b\u003e:Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:Greater than 98% as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 0.02 ng\/µg (0.2 EU\/µg) as determined by LAL test.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:Measured in a cell proliferation assay using BALB\/c 3T3 cells. The ED50 for this effect is 5-20 ng\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRedissolve\u003c\/b\u003e:Always centrifuge tubes before opening.Do not mix by vortex or pipetting.\nIt is not recommended to reconstitute to a concentration less than 100μg\/ml.\nDissolve the lyophilized protein in distilled water.\nPlease aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks.\nReconstituted protein solution can be stored at 4-7°C for 2-7 days.\nAliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDelivery condition\u003c\/b\u003e:The product is shipped at ambient temperature.\nUpon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF\/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48647909015652,"sku":"EPT197","price":368.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_b68dc91d-3f8a-4574-82a0-abea81c0fa0a.jpg?v=1782704618","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/recombinant-human-pdgf-bb","provider":"GeneBio ","version":"1.0","type":"link"}