{"product_id":"recombinant-human-n6amt1-hemk2-protein-his-tag","title":"Recombinant Human N6AMT1\/HEMK2 Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: C21orf127;HEMK2;MTQ2;N6AMT;PRED28;m.HsaHemK2P\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Epigenetics and Nuclear Signaling\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: E.coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q9Y5N5\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: AAH11554.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Pyruvate  dehydrogenase kinase, isozyme 1, also known as [Pyruvate dehydrogenase  [lipoamide]] kinase isozyme 1, mitochondrial and PDK1, is a member of the PDK \/  BCKDK protein kinase family. PDK-1 is expressed predominantly in the heart. It  contains onehistidine kinase domain. Pyruvate dehydrogenase kinase (PDK)  isoforms are molecular switches that downregulate the pyruvate dehydrogenase  complex (PDC) by reversible phosphorylation in mitochondria. An inhibitory  effect of lipoic acid on PDKs would result in less phosphorylation of E1 and  hence increased PDC activity. At least two isoenzymic forms of pyruvate  dehydrogenase kinase ( PDK-1 and PDK-2 ) may be involved in the regulation of  enzymatic activity of mammalian pyruvate dehydrogenase complex by  phosphorylation. PDK-3 appears to have the highest specific activity among the  three isoenzymes. PDK-1 inhibits the mitochondrial pyruvate dehydrogenase  complex by phosphorylation of the E1 alpha subunit, thus contributing to the  regulation of glucose metabolism.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Ser 186\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 20mM Tris, pH 8.0\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e: Please contact us for more information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 21.2 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 23 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972851777636,"sku":"PKSH030850","price":466.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_4c544955-5c05-445c-abb2-73aa40540049.jpg?v=1772200561","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/recombinant-human-n6amt1-hemk2-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}