{"product_id":"recombinant-human-m-csf-protein","title":"Recombinant Human M-CSF protein","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:2 ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150 a.a. portion that is necessary and sufficient for interaction with the receptor. The first 223 a.a. of mature human M-CSF shares 88 %, 86 %, 81 % and 74 % sequence identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch_area\u003c\/b\u003e:Immunology\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:\u0026gt; 95 % by SDS-PAGE and HPLC analyses.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:Escherichia coli.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFull product name\u003c\/b\u003e:Recombinant Human Macrophage Colony Stimulating Factor\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse M-NFS-6 cells is less than 1 ng\/ml, corresponding to a specific activity of \u0026gt; 1. × 16 IU\/mg.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e:M-CSF, CSF1,CSF-1, MCSFHuman\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 1 EU\/μg of rHuM-CSF as determined by LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:-20ºC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGS\u003c\/p\u003e\u003cp\u003e\u003cb\u003euniprot accession\u003c\/b\u003e:P963\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:Approximately 36.8 kDa, a disulfide-linked homodimer consisting of two 158 amino acid polypeptide chains.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:Lyophilized from a .2 μm filtered solution in PBS, pH7.4.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48653997342820,"sku":"CD01312","price":277.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_cc08fd53-2562-4db5-b6ff-bcadca7399af.jpg?v=1782767255","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/recombinant-human-m-csf-protein","provider":"GeneBio ","version":"1.0","type":"link"}