{"product_id":"recombinant-human-c-kit-cd117-protein-fc-tag","title":"Recombinant Human c-KIT\/CD117 Protein (Fc Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: CD117;Mast\/stem cell growth factor receptor Kit;PBT;Piebald trait protein;Proto-oncogene c-Kit;SCFR;Tyrosine-protein kinase Kit;p145 c-kit;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Immunology,Tags \u0026amp; Cell Markers,Signal Transduction,Neuroscience,Stem Cells,Cancer,Developmental Biology\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: HEK293 Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-hFc\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: P10721\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: P10721-2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor; also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily; tyr protein kinase family and CSF-1\/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs; c-Kit staining is typically cytoplasmic; with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors; mast cell disease; acute myelogenous lukemia; and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Immobilized human KIT-Fc at 10 μg\/ml (100 μl\/well) can bind biotinylated mouse KITL-His (1-189), The EC50 of biotinylated mouse KITL-His (1-189)) is 2. 87-6. 71 ng\/ml. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Thr516\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 95 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile PBS, pH 7.4 \u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 82.0 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 93-115 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972859445348,"sku":"PKSH030941","price":466.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_793eff43-2614-445f-8871-17a8e631c85b.jpg?v=1772201000","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/recombinant-human-c-kit-cd117-protein-fc-tag","provider":"GeneBio ","version":"1.0","type":"link"}