{"product_id":"phospho-rasgrf1-ser916-antibody-cat-csb-pa256747","title":"Phospho-RASGRF1 (Ser916) Antibody - Cat. #: CSB-PA256747","description":"\u003cp\u003e\u003cb\u003eSize \u003c\/b\u003e:100ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone Number\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAliases\u003c\/b\u003e:CDC25 antibody; CDC25L antibody; GNRP antibody; GRF1 antibody; GRF55 antibody; Guanine nucleotide exchange factor antibody; Guanine nucleotide releasing factor 55 kD antibody; Guanine nucleotide releasing protein antibody; guanine nucleotide-releasing factor 1 antibody; Guanine nucleotide-releasing protein antibody; H GRF55 antibody; HGRF55 antibody; PP13187 antibody; Ras protein specific guanine nucleotide releasing factor 1 antibody; Ras specific guanine nucleotide releasing factor antibody; Ras specific guanine nucleotide releasing factor CDC25 homolog antibody; Ras specific nucleotide exchange factor CDC25 antibody; Ras-GRF1 antibody; Ras-specific guanine nucleotide-releasing factor 1 antibody; Ras-specific nucleotide exchange factor CDC25 antibody; RASGRF1 antibody; RGRF1_HUMAN antibody\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Type\u003c\/b\u003e:Polyclonal Antibody\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen Species\u003c\/b\u003e:Homo sapiens (Human)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt ID\u003c\/b\u003e:Q13972\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e:Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1.\n\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRaised in\u003c\/b\u003e:Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e:Mouse, Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Applications\u003c\/b\u003e:ELISA, IHC; IHC:1:50-1:100\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003eRas activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.\u003cbr\u003e\u003cbr\u003eHuibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285. \u003cbr\u003eHuibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189. \u003cbr\u003eRaymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379. \u003cbr\u003eTie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e:IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification Method\u003c\/b\u003e:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e:Non-conjugated\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e:liquid\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStroage\u003c\/b\u003e:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Names\u003c\/b\u003e:RASGRF1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:Signal transduction\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40484028973156,"sku":"CSB-PA256747","price":621.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_444f5c08-f459-4195-8f91-6aae75e541be.jpg?v=1660086425","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/phospho-rasgrf1-ser916-antibody-cat-csb-pa256747","provider":"GeneBio ","version":"1.0","type":"link"}