{"product_id":"phi29-dna-polymerase","title":"Phi29 DNA Polymerase","description":"\u003cspan class=\"author\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Details\u003c\/strong\u003e\u003c\/span\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis product is a 74.4 kDa purified recombinant protein inducibly expressed in\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003cem data-mce-fragment=\"1\"\u003eE. coli\u003c\/em\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003ecarrying the φ29 DNA polymerase gene. With high sensitivity and great synthesis ability, it can be used for whole genome amplification from a single cell. It possesses 3'→5' proofreading exonuclease activity to ensure high fidelity of resulting DNA. Its properties of multiple strand displacement and processive synthesis allow \u0026gt;10 kb lengths of amplification products from genome or plasmid DNA, and enable rolling-circle replication of circular DNA.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eHighlights\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e· Isothermal amplification;\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e· High fidelity; high efficiency; high sensitivity; high yield;\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e· Random primers or specific N9 primers can be used.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eSequencing, SNP genotyping, STR\/ microsatellite analysis, virus detection, miRNA detection, etc.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eat -20°C  for two years\u003cbr data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eShipping\u003c\/strong\u003e\u003cbr data-mce-fragment=\"1\"\u003eDry ice (-70 ℃)\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"clear\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan class=\"author\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"firstRow\" data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eLP101-01 \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eLP101-02 \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePhi29 DNA Polymerase\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e250 units\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e5×250 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10×Phi29 DNA Polymerase Buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e250 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003ePhi29 Random Primers\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e125 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e625 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10 mM dNTPs\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e25 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e125 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10×DNA Loading Buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"GeneBio Systems","offers":[{"title":"250 units","offer_id":41977681805412,"sku":"LP101-01","price":79.0,"currency_code":"GBP","in_stock":false},{"title":"5×250 units","offer_id":41977681838180,"sku":"LP101-02","price":313.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_431f75e9-c580-45b0-85c9-56ee7a00871a.jpg?v=1691001144","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/phi29-dna-polymerase","provider":"GeneBio ","version":"1.0","type":"link"}