{"product_id":"human-gamma-glutamylcysteine-synthetase-gcs-elisa-kit","title":"Human Gamma-glutamylcysteine synthetase (?-GCS) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e:Human (Homo sapiens)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt\u003c\/b\u003e:N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e:?-GCS\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:glutamate-cysteine ligase; gamma-glutamylcysteine synthetase\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRange\u003c\/b\u003e:Request Information\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:Request Information\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIntra-AssayCV\u003c\/b\u003e:?5.2%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eInter-AssayCV\u003c\/b\u003e:?9.6%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecovery\u003c\/b\u003e:1.05\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample Type\u003c\/b\u003e:Serum, Plasma, Other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Method\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAnalysis Method??\u003c\/b\u003e:Quantitive\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e:This assay employs a two-site sandwich ELISA to quantitate ?-GCS in samples. An antibody specific for ?-GCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any?-GCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ?-GCS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ?-GCS bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Overview\u003c\/b\u003e:Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.It catalyses the ATP-dependent condensation of cysteine and glutamate to form the dipeptide gamma-glutamylcysteine. The peptide bond in this peptide product is between the carboxylate group of the glutamate and the amino group of the cysteine.The product of the GCL-mediated condensation reaction of L-glutamate and L-cysteine is gamma-glutamylcysteine, which is readily condensed with glycine, by glutathione synthetase, to form glutathione.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40500710867044,"sku":"AE62809HU","price":849.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_e828917c-d295-46cb-aaee-9b264b83c596.jpg?v=1660529581","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/human-gamma-glutamylcysteine-synthetase-gcs-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}