{"product_id":"hipure-viral-rna-kit","title":"HiPure Viral RNA Kit","description":"\u003ch3\u003eIntroduction\u003c\/h3\u003e\n\u003cp\u003eHipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A\/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.\u003c\/p\u003e\n\u003ch3\u003eDetails\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFeatures\u003c\/td\u003e\n\u003ctd\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Functions\u003c\/td\u003e\n\u003ctd\u003eExtract viral RNA from 140μl cell-free samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eRT-PCR, Northern hybridization, and various virus detection\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification method\u003c\/td\u003e\n\u003ctd\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification technology\u003c\/td\u003e\n\u003ctd\u003eSilica technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcess method\u003c\/td\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eCell-free body fluid or culture medium\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample amount\u003c\/td\u003e\n\u003ctd\u003e140μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume\u003c\/td\u003e\n\u003ctd\u003e≥15μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTime per run\u003c\/td\u003e\n\u003ctd\u003e≤25 minutes\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd\u003e800μl\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd\u003e100μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch1\u003e\n\u003cbr\u003ePrinciple\u003c\/h1\u003e\n\u003cp\u003eHipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.\u003c\/p\u003e\n\u003cp\u003eThe sample is homogenized and lysed in the lysate, and RNA is released into the lysate. The high concentration of guanidine isothiocyanate contained in the lysate denatured and inactivated endogenous or exogenous RNase, while RNA is protected from degradation. The lysate is centrifuged to remove insoluble impurities. After adding ethanol to adjust the binding conditions, it is transferred to the column for filtration. RNA is adsorbed on the membrane of the column, while protein is removed without adsorption. The column is washed with buffer VHB to remove protein and other impurities, washed with buffer RW2 to remove salt. Finally the RNA is eluted by RNase free water. The eluted RNA can be directly used in RT-PCR, Northern blot, poly-A purification, in vitro translation, etc.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdvantages\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFast - several samples can be extracted in 20 minutes by column method\u003c\/li\u003e\n\u003cli\u003eHigh quality - high purity total RNA can be directly used in various sensitive downstream applications\u003c\/li\u003e\n\u003cli\u003eSafe - no phenol chloroform extraction required\u003c\/li\u003e\n\u003cli\u003eSensitive - RNA can be recovered at the level of PG\u003c\/li\u003e\n\u003cli\u003eHigh yield - carrier RNA contained in the product maximize the recovery of trace nucleic acid\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eContents\u003c\/td\u003e\n\u003ctd\u003eR417102\u003c\/td\u003e\n\u003ctd\u003eR417103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification Times\u003c\/td\u003e\n\u003ctd\u003e50 Preps\u003c\/td\u003e\n\u003ctd\u003e250 Preps\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiPure Viral Micro Columns\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003ctd\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2ml Collection Tubes\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e100\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer VRL\u003c\/td\u003e\n\u003ctd\u003e50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e200 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCarrier RNA\u003c\/td\u003e\n\u003ctd\u003e310 µg\u003c\/td\u003e\n\u003ctd\u003e3 x 310 µg\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer VHB*\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e13 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e110 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW2*\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e20\u003cspan\u003e \u003c\/span\u003eml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e2 x 50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease Free Water\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e10 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e30 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCarrier RNA should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions\u003c\/p\u003e\n\u003ch3\u003eArticles\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/journals.asm.org\/doi\/abs\/10.1128\/JVI.00667-20\" data-mce-href=\"https:\/\/journals.asm.org\/doi\/abs\/10.1128\/JVI.00667-20\"\u003e【JOURNAL OF VIROLOGY】L226Q Mutation on Influenza H7N9 Virus Hemagglutinin Increases Receptor-Binding Avidity and Leads to Biased Antigenicity Evaluation | Journal of Virology\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2095809918309366\" data-mce-href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2095809918309366\"\u003e【Engineering】Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2021.673872\/full\" data-mce-href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2021.673872\/full\"\u003e【Frontiers in Microbiology】Development of a High-Efficiency Immunomagnetic Enrichment Method for Detection of Human Norovirus via PAMAM Dendrimer\/SA-Biotin Mediated Cascade-Amplification\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956713521002991\" data-mce-href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956713521002991\"\u003e【FOOD CONTROL】Detection of group A rotavirus in oyster tissues by in situ capture RT-qPCR\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/www.mdpi.com\/950828\" data-mce-href=\"https:\/\/www.mdpi.com\/950828\"\u003e【Viruses-Basel】Library Preparation Based on Transposase Assisted RNA\/DNA Hybrid Co-Tagmentation for Next-Generation Sequencing of Human Noroviruses\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003ca href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/jmv.28216\" data-mce-href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/jmv.28216\"\u003e【JOURNAL OF MEDICAL VIROLOGY】GII.17[P17] and GII.8[P8] noroviruses showed different RdRp activities associated with their epidemic characteristics\u003c\/a\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cbr\u003e\u003c\/h3\u003e","brand":"GeneBio Systems","offers":[{"title":"50 preps","offer_id":41740388040804,"sku":"R417102","price":121.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/hipureviralrnakitimage.png?v=1683470041","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/hipure-viral-rna-kit","provider":"GeneBio ","version":"1.0","type":"link"}