{"product_id":"hipure-dna-rna-protein-kit","title":"HiPure DNA\/RNA\/Protein Kit","description":"\u003cp\u003e\u003ca href=\"https:\/\/www.magen-tec.com\/products\/info.aspx?itemid=108\" data-slide-index=\"0\"\u003e1\u003c\/a\u003e\u003c\/p\u003e\n\u003ch1\u003eHiPure DNA\/RNA\/Protein Kit\u003c\/h1\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eIntroduction\u003c\/h2\u003e\n\u003cp\u003eHiPure DNA \/ RNA \/ protein kit is the most rapid and simple method to simultaneously extract DNA, RNA and protein from cultured cells and animal soft tissue samples. This kit adopts column technology which is no need for dangerous phenol chloroform extraction and time-consuming alcohol precipitation in the extraction process. This kit is suitable for simultaneous extraction of RNA, DNA and protein from \u0026lt; 1x 10\u003csup\u003e^\u003c\/sup\u003e7 eukaryotic cultured cells as well as from animal soft tissues less than 20mg such asliver, kidney, spleen and brain. The purified RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection, in vitro translation while the obtained DNA can be directly used in PCR and Southern hybridization, the obtained protein can be directly used in SDS-PAGE electrophoresis, western blot and so on.\u003c\/p\u003e\n\u003ch1\u003eDetails\u003c\/h1\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eMain Functions\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eCo-isolation DNA and RNA \/protein from a single sample (cells, soft tissue, plant sample)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eSDS-PAGE electrophoresis and western blot, etc\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003ePurification method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eMini spin column\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003ePurification technology\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eSilica technology\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eProcess method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eManual (centrifugation or vacuum)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eCulture cells, animal tissues, plant fungi, yeast, bacteria and other samples\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003eSample amount\u003c\/span\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eCultured cells: \u0026lt; 10\u003csup\u003e^\u003c\/sup\u003e7\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003eAnimal tissue: ≤\u003cspan lang=\"EN-US\"\u003e 20 mg\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan lang=\"EN-US\"\u003ePlant samples: ≤ 150 mg\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan lang=\"EN-US\"\u003eYeast cells: 2 x 10\u003csup\u003e^\u003c\/sup\u003e6 - 5 x 10\u003csup\u003e^\u003c\/sup\u003e7\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch1\u003e\n\u003cbr\u003ePrinciple\u003c\/h1\u003e\n\u003cp\u003e\u003cspan\u003eThe Kit isolates total RNA from up to 10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e \u003c\/span\u003e7\u003c\/sup\u003e\u003cspan\u003e cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eAdvantages\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh quality - high purity total RNA \/ DNA can be directly used in a variety of downstream applications\u003c\/li\u003e\n\u003cli\u003eFast - column method can complete the extraction of several samples in 30 minutes\u003c\/li\u003e\n\u003cli\u003eSafe - no phenol chloroform extraction required\u003c\/li\u003e\n\u003cli\u003eSimultaneous extraction- simultaneously isolate DNA and RNA from one sample\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eContents\u003c\/td\u003e\n\u003ctd\u003eR521102\u003c\/td\u003e\n\u003ctd\u003eR521103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNumber of Purification\u003c\/td\u003e\n\u003ctd\u003e50 Preps\u003c\/td\u003e\n\u003ctd\u003e250 Preps\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiPure DNA Mini Columns II\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003ctd\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiPure RNA Mini Columns\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003ctd\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2ml Collection Tubes\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e100\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e500\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RL\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e250 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer DW1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e30 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e150 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW1\u003c\/td\u003e\n\u003ctd\u003e50 ml\u003c\/td\u003e\n\u003ctd\u003e250 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW2*\u003c\/td\u003e\n\u003ctd\u003e2 x 20  ml\u003c\/td\u003e\n\u003ctd\u003e3 x 50  ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase Free Water\u003c\/td\u003e\n\u003ctd\u003e10 ml\u003c\/td\u003e\n\u003ctd\u003e30\u003cspan\u003e \u003c\/span\u003eml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd\u003e10 ml\u003c\/td\u003e\n\u003ctd\u003e30 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDPS Solution (5% SDS)\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e30 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e150 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eHipure DNA\/RNA\/protein kit can be stored at room temperature (15-25℃) in dry for 18 months. At low temperature, buffer RL may form precipitation. It needs 55℃\u003cspan\u003e \u003c\/span\u003ewater bath to completely dissolve the precipitation before use. As DEPC treated water does not contain any bacteriostatic factors, it may be contaminated by bacterial or fungal when placed or operated at room temperature. We recommend to pack DEPC treated water separately and store it at -20℃\u003cspan\u003e \u003c\/span\u003eto reduce pollution. If DEPC treated water is polluted, please prepare it again.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R5211_HiPure_DNA_RNA_Protein_Kit_2022-GBS.pdf?v=1700843221\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"188\" height=\"53\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 preps","offer_id":41450354180196,"sku":"R521102","price":325.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/Screenshot_33_2ed97dad-2bdf-4f5c-83ff-a8d661880826.png?v=1674666302","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/hipure-dna-rna-protein-kit","provider":"GeneBio ","version":"1.0","type":"link"}