{"product_id":"anti-caspase-14-casp14-antibody-picoband-r","title":"Anti-Caspase-14\/CASP14 Antibody Picoband®","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100 μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e: Lyophilized\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human,Mouse,Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e: IHC,WB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication Details\u003c\/b\u003e: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg\/ml, Human\u003cbr\u003eWestern blot, 0.1-0.5μg\/ml, Human, Mouse, Rat\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Name\u003c\/b\u003e: CASP14\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: No cross reactivity with other proteins.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Caspase 14 is an enzyme that in humans is encoded by the CASP14 gene. CASP14 belongs the evolutionarily conserved caspase family. CASP14 was assigned to chromosome 19p13.1. Using Northern blot and semiquantitative RT-PCR analyses, it was showed that CASP14 was transcriptionally upregulated during maintenance of confluent cultures of human epidermal keratinocytes. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. Caspase 14 seems to play a role in keratinocyte differentiation and cornification and probably regulates maturation of the epidermis by proteolytically processing filaggrin.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e: A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-14, different from the related rat and mouse sequences by four amino acids.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e: Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eContents\u003c\/b\u003e: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.\u003cbr\u003e\n*This antibody is supplied in a stabilized formulation. \nCompatibility with conjugation reactions depends on the chemistry of the conjugation method used. \nFor conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification\u003c\/b\u003e: Immunogen affinity purified.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Add 0.2ml of distilled water will yield a concentration of 500ug\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReference\u003c\/b\u003e: 1. Denecker, G., Hoste, E., Gilbert, B., Hochepied, T., Ovaere, P., Lippens, S., Van den Broecke, C., Van Damme, P., D'Herde, K., Hachem, J.-P., Borgonie, G., Presland, R. B., Schoonjans, L., Libert, C., Vandekerckhove, J., Gevaert, K., Vandenabeele, P., Declercq, W. Caspase-14 protects against epidermal UVB photodamage and water loss. Nature Cell Biol. 9: 666-674, 2007. \n2. Eckhart, L., Ban, J., Fischer, H., Tschachler, E. Caspase-14: analysis of gene structure and mRNA expression during keratinocyte differentiation. Biochem. Biophys. Res. Commun. 277: 655-659, 2000.\n3. Van de Craen, M., Van Loo, G., Pype, S., Van Criekinge, W., Van den brande, I., Molemans, F., Fiers, W., Declercq, W., Vandenabeele, P. Identification of a new caspase homologue: caspase-14. Cell Death Differ. 5: 838-846, 1998.\n\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot ID\u003c\/b\u003e: P31944\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost\u003c\/b\u003e: Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: Adding 0.2 ml of distilled water will yield a concentration of 500 μg\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eCross Reactivity\u003c\/b\u003e: No cross-reactivity with other proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Rabbit IgG\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePhospho_site\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone Number\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eObserved Molecular Weight\u003c\/b\u003e: 28 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated Molecular Weight\u003c\/b\u003e: 27680 MW\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e: 23581\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProtein Name\u003c\/b\u003e: Caspase-14\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Full Name\u003c\/b\u003e: caspase 14\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e: caspase 14; caspase-14; ARCI12; caspase14; CASP14; CASP 14; CASP-14\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48788104511588,"sku":"PA2104","price":431.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_041366e4-7aa9-4d2b-a88b-ed1fd7a44137.jpg?v=1784407814","url":"https:\/\/www.genebiosystems.com\/en-gb\/products\/anti-caspase-14-casp14-antibody-picoband-r","provider":"GeneBio ","version":"1.0","type":"link"}