{"product_id":"recombinant-salmonella-typhi-dna-gyrase-subunit-bgyrb-partial","title":"Recombinant Salmonella typhi DNA gyrase subunit B(gyrB),partial","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100ug.  Other sizes are also available. For further information, please contact us.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot ID\u003c\/b\u003e:P0A2I4\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Names\u003c\/b\u003e:gyrB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOrganism\u003c\/b\u003e:Salmonella typhi\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAA Sequence\u003c\/b\u003e:PALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATLHANAHAPALSGEALEKLVSEYNATQKMIGRMERRFPKALLKELVYQPTLTEADLSDEQTVTRWVNALITELNEKEQHGSQWKFDVHTNTEQNLFEPIVRVRTHGVDTDYPLDHEFVTGAEYRRICTLGEKLRGLIEEDAFIERGERRQPVTSFEQALEWLVKESRRGLAIQRYKGLGEMNPDQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAANIDI\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Region\u003c\/b\u003e:415-804aa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence Info\u003c\/b\u003e:partial\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSource\u003c\/b\u003e:E.coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTag Info\u003c\/b\u003e:N-terminal 10xHis-tagged and C-terminal Myc-tagged\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMW\u003c\/b\u003e:51.8 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Name(s)\u003c\/b\u003e:\/\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRelevance\u003c\/b\u003e:A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReference\u003c\/b\u003e:\"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18.\"\nParkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P.  Barrell B.G.\nNature 413:848-852(2001)\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:Greater than 85% as determined by SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e:Liquid or Lyophilized powder\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:If the delivery form is liquid, the default storage buffer is Tris\/PBS-based buffer, 5%-50% glycerol.\nIf the delivery form is lyophilized powder, the buffer before lyophilization is Tris\/PBS-based buffer, 6% Trehalose, pH 8.0.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg\/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?\/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. \nGenerally, the shelf life of liquid form is 6 months at -20?\/-80?. The shelf life of lyophilized form is 12 months at -20?\/-80?.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eNotes\u003c\/b\u003e:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFunction\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eInvolvement in disease\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSubcellular Location\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProtein Families\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTissue Specificity\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePaythway\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHGNC Database Link\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniGene Database Link\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eKEGG Database Link\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSTRING Database Link\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOMIM Database Link\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eLead Time Guidance\u003c\/b\u003e:3-7 business days\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":40529795580004,"sku":"CSB-EP358232SWW","price":804.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_5ecf0285-411d-4c39-b2ab-44841376c40e.jpg?v=1661101892","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/recombinant-salmonella-typhi-dna-gyrase-subunit-bgyrb-partial","provider":"GeneBio ","version":"1.0","type":"link"}