{"product_id":"recombinant-human-stim1-gok-protein-his-tag","title":"Recombinant Human STIM1\/GOK Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: D11S4896E;GOK;IMD10;STRMK;TAM;TAM1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction;Cancer;metabolism;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: HEK293 Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q13586\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_003147.2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Stromal interaction molecule 1, also  known as STIM1 and GOK, is a cell membrane, a single-pass type I membrane  protein and a endoplasmic reticulum membrane protein. STIM1 \/ GOK is  ubiquitously expressed in various human primary cells and tumor cell lines. It  contains oneEF-hand domain and oneSAM (sterile alpha motif) domain.  STIM1 \/ GOK plays a role in mediating Ca2+influx following depletion of  intracellular Ca2+stores. It acts as Ca2+sensor in the  endoplasmic reticulum via its EF-hand domain. Upon Ca2+depletion,  STIM1 \/ GOK translocates from the endoplasmic reticulum to the plasma membrane  where it activates the Ca2+release-activated Ca2+(CRAC) channel subunit,  TMEM142A \/ ORAI1. Transfection of STIM1 \/ GOK into cells derived from a  rhabdoid tumor and from a rhabdomyosarcoma that do not express detectable  levels of STIM1 can induce cell death, suggesting a possible role in the control  of rhabdomyosarcomas and rhabdoid tumors. Defects in STIM1 are the cause of  immune dysfunction with T-cell inactivation due to calcium entry defect type 2  (IDTICED2) which is an immune disorder characterized by recurrent infections,  impaired T-cell activation and proliferative response, decreased T-cell  production of cytokines, lymphadenopathy, and normal lymphocytes counts and  serum immunoglobulin levels.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Asp 213\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 97 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile PBS, pH 7.4\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 23.3 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 33-38 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47973005918308,"sku":"PKSH031120","price":540.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_3aab49ae-61a4-4ee6-8136-8b26fcca741d.jpg?v=1772201930","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/recombinant-human-stim1-gok-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}