{"product_id":"recombinant-human-kynu-kynureninase-protein-his-tag","title":"Recombinant Human KYNU\/Kynureninase Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: KYNUU\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Signal Transduction;metabolism;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Enzyme\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q16719\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: Q16719\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Protein disulfide-isomerase,  also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase  subunit beta, p55 and P4HB, is a peripheral membrane protein which belongs to  the protein disulfide isomerase family. P4HB is highly abundant. In some  cell types, it seems to be also secreted or associated with the plasma  membrane, where it undergoes constant shedding and replacement from  intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid  cell surfaces. It is identified by mass spectrometry in melanosome fractions  from stage I to stage IV. P4HB reduces and may activate fusogenic properties of  HIV-1 gp120 surface protein, thereby enabling HIV-1 entry into the cell. P4HB  catalyzes the formation, breakage and rearrangement of disulfide bonds. At the  cell surface, it seems to act as a reductase that cleaves disulfide bonds of  proteins attached to the cell. P4HB may therefore cause structural  modifications of exofacial proteins. Inside the cell, it seems to form\/rearrange  disulfide bonds of nascent proteins. At high concentrations, P4HB functions as  a chaperone that inhibits aggregation of misfolded proteins. At low  concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may  be involved with other chaperones in the structural modification of the TG  precursor in hormone biogenesis. It also acts a structural subunit of various  enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer  protein MTTP.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Measured by its ability to oxidize 3-hydroxykynurenine. The specific activity is \u0026gt; 200 pmoles\/min\/μg. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1- Asn 465\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 92 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 25% glycerol\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 53.7 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 47 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47973022859364,"sku":"PKSH031397","price":838.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_74fce769-5342-4c53-b803-65890bdb3846.jpg?v=1772203284","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/recombinant-human-kynu-kynureninase-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}