{"product_id":"recombinant-human-gad65-gad2-gad-2-protein-gst-tag","title":"Recombinant Human GAD65\/GAD2\/GAD-2 Protein (GST Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: GAD65\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Neuroscience;Cancer;metabolism\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-GST\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q05329\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_000809.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Glutamate decarboxylase 2, also  known as glutamate decarboxylase 65 kDa isoform, 65 kDa glutamic acid  decarboxylase, GAD2 and GAD65, is a member of thegroup II decarboxylase  family. GAD2 is identified as a major autoantigen in insulin-dependent  diabetes. GAD2 is responsible for catalyzing the production of  gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme  has been identified in the human pancreas since it has been identified as an  autoantibody and an autoreactive T cell target in insulin-dependent diabetes.  GAD2 may also play a role in the stiff man syndrome. GAD2 is implicated in the  formation of the gamma-aminobutyric acid (GABA), a neurotransmitter involved in  the regulation of food intake. GABA is synthesized in brain by two isoforms of  glutamic acid decarboxylase (Gad), GAD1 and GAD2. GAD1 provides most of the  GABA in brain, but GAD2 can be rapidly activated in times of high GABA demand.  Mice lacking GAD2 are viable whereas deletion of GAD1 is lethal. Deletion of  GAD2 increased ethanol palatability and intake and slightly reduced the  severity of ethanol-induced withdrawal.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Leu 585\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 90 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 200 Tirs, 150 mM NaCl, pH 8.0, 10 % glycerol, 1 mM GSH \u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 92.6 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: \u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972814651492,"sku":"PKSH030280","price":1084.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_096b16ed-cad1-4850-9195-5a001c6695f3.jpg?v=1772197461","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/recombinant-human-gad65-gad2-gad-2-protein-gst-tag","provider":"GeneBio ","version":"1.0","type":"link"}