{"product_id":"rat-8-hydroxy-desoxyguanosine-8-ohdg-elisa-kit-1","title":"Rat 8-Hydroxy-desoxyguanosine (8-OHdG) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96T. Other sizes are also available. Please Inquire.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies Reactivity\u003c\/b\u003e:Rat (Rattus norvegicus)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt\u003c\/b\u003e:N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e:8-OHdG\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:N\/A\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:ELISA\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRange\u003c\/b\u003e:0.312-20 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:0.078 ng\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIntra-AssayCV\u003c\/b\u003e:≤4.6%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eInter-AssayCV\u003c\/b\u003e:≤11.3%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecovery\u003c\/b\u003e:1,05\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample Type\u003c\/b\u003e:Serum, Plasma, Other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Method\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAnalysis Method\u003c\/b\u003e:Quantitive\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e:This assay employs a two-site sandwich ELISA to quantitate 8-OHdG in samples. An antibody specific for 8-OHdG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any8-OHdG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 8-OHdG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 8-OHdG bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProduct Overview\u003c\/b\u003e:8-oxo-7,8-dihydro-2 deoxyguanosine(8-OHdG), is probably the most important product of \"oxidative stress” in DNA. Its concentration in DNA is, in fact. a quantitative analysis of the degree of DNA damage that an organism has undergone. Due to the importance of 8-OHdG of nucleic acidg in mutagenesis, carcinogenesis and aging, numerous chemical and biological investigations have been made on this subject in the past time. Kuchino and co-workers have found that 8-OHdG residue in DNA is misreading during the process of DNA replication. Recently, some reports have been presented on high 8-OHdG levels in patients suffering from various diseases such as chronic hepatitis, Fanconi s anemia, diabetes mellitus and Helicobacter pylori infections. As a result, 8-OHdG is a useful marker for the study of DNA damage arising from reactive oxygen species and is of great significance for cancer research.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39792306159716,"sku":"AE22964RA","price":970.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/no_image_default_image-jpeg_d7cc4a35-b1a7-4347-956e-917a9fbae62c.jpg?v=1659152316","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/rat-8-hydroxy-desoxyguanosine-8-ohdg-elisa-kit-1","provider":"GeneBio ","version":"1.0","type":"link"}