{"product_id":"mrna-capping-kit","title":"mRNA Capping Kit","description":"\u003cspan class=\"author\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Details\u003c\/strong\u003e\u003c\/span\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis product contains vaccinia virus capping enzyme, mRNA cap structure 2'-O-methyltransferase and other capping reaction components, which can be used for 5' capping modification of RNA produced by T7 in vitro transcription (JT101).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIn eukaryotes, after transcription to form the original mRNA, a special cap structure needs to be formed at the 5' end. This structure plays an important role in the stability, transport (exit) and translation of mRNA. The capping modification of the 5' end of RNA by the enzymatic reaction of capping enzymes is a simple and effective method: Vaccinia Capping Enzyme can attach 7-methylguanylate cap (m7Gppp, Cap0) to the 5' end of RNA to form m7Gppp5'N-mRNA (Cap0-mRNA). The mRNA Cap 2 '-O-methyltransferas uses CAP0-mRNA as substrate and SAM (S-adenosine methionine) as a methyl donor to methylate 2' -oh of the first nucleotide of cap0-mrna 5 'end adjacent to the cap structure to form Cap1-mRNA. \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eCap1 structure can improve mRNA stability, which helps to enhance its expression ability in cell transfection and microinjection experiments.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis product uses vaccinia virus capping enzyme and mRNA Cap-2'-O-methyltransferase at the same time in the capping reaction, so that the capping reaction can be completed in the same reaction. The reaction product is Cap1-mRNA, and the capping efficiency can be close to 100% in the correct cap orientation.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e-20℃ for one year, avoid repeated freezing and thawing.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eShipping\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDry ice (-70 ℃)\u003c\/p\u003e\n\u003cdiv class=\"clear\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003cdiv class=\"clear\" data-mce-fragment=\"1\"\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan class=\"author\" data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\n\u003cdiv class=\"acontentc\" data-mce-fragment=\"1\"\u003e\n\u003ctable border=\"1\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr class=\"firstRow\" data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eLC101-01 (25 rxns) \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eLC101-02 (100 rxns) \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eVaccinia Capping Enzyme\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e20 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e80 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003emRNA Cap 2’-O-Methyltransferase\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e40 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e160 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10×Capping Reaction Buffer\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e200 μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eGTP (10 mM)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e50 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e200 μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eSAM (32 mM)\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e40 μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eRibonuclease Inhibitor\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e10 μl\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e40 μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eRNase-free Water\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e1 ml\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e2×1 ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"GeneBio Systems","offers":[{"title":"25 rxns","offer_id":41977732825188,"sku":"LC101-01","price":499.95,"currency_code":"EUR","in_stock":true},{"title":"100 rxns","offer_id":41977732857956,"sku":"LC101-02","price":1665.95,"currency_code":"EUR","in_stock":true}],"url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/mrna-capping-kit","provider":"GeneBio ","version":"1.0","type":"link"}