{"product_id":"lh-elisa-kit-for-mouse","title":"LH ELISA kit (Mouse)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 96Tests\u003c\/p\u003e\u003cp\u003e\u003cb\u003e# of Times Cited in literature\u003c\/b\u003e: 58\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePrepare Time\u003c\/b\u003e: 1-3 days(please inquire for mutiple units)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Name\u003c\/b\u003e: LH\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Full Name\u003c\/b\u003e: Luteinizing Hormone\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e: ICSH; Lutropin; Interstitial Cell Stimulating Hormone\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot\u003c\/b\u003e: P01216 \u0026amp; O09108\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e: 12640\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series\u003c\/b\u003e: CE kit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFeatured Series Function\u003c\/b\u003e: Detects small molecule\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Reactive with Mouse LH \/ Luteinizing Hormone\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMethod\u003c\/b\u003e: Colormetric\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection principle\u003c\/b\u003e: Competitive Inhibition\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection\nrange\u003c\/b\u003e: 370.4-30,000pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e: 145.2pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Time\u003c\/b\u003e: 2h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample Size\u003c\/b\u003e: 50uL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRecommended\/Predicted\nSample Types\u003c\/b\u003e: Serum, Plasma and other Biological Fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Precision\u003c\/b\u003e: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReproducibility test menthod\u003c\/b\u003e: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.\nCV(%) = SD\/meanX100\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShelf-life\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH).\nNo significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStability\u003c\/b\u003e: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay procedure summary\u003c\/b\u003e: 1. Prepare all reagents, samples and standards;\n2. Add 50µL standard or sample to each well.\n    And then add 50µL prepared Detection Reagent A immediately.\n    Shake and mix. Incubate 1 hour at 37°C;\n3. Aspirate and wash 3 times;\n4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\n5. Aspirate and wash 5 times;\n6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\n7. Add 50µL Stop Solution. Read at 450 nm immediately.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone (LH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone (LH) and unlabeled Luteinizing Hormone (LH) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone (LH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e: Endocrinology;Reproductive science;Hormone metabolism;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReferences Citing This Product\u003c\/b\u003e: \u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19686804\"\u003eEffects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes.\u003c\/a\u003e\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21108604\"\u003ePPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0303720709004043\"\u003eEffects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24486453\"\u003eInhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell lines\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/pubs.rsc.org\/en\/content\/articlehtml\/2013\/tb\/c3tb20693a\"\u003eNanocomposites of bio-based hyperbranched polyurethane\/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0006291X14001843\"\u003eCritical function of RA-GEF-2\/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25505846\"\u003eTargeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26771609\"\u003eOverexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26977770\"\u003eA Jacob\/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27803384%20\"\u003eExposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28232269\"\u003eNew gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency.\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28731686\"\u003ePyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28958147\"\u003eDevelopment of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29149324\"\u003ePyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1\/2 Signaling in LbT2 Mouse Pituitary Cells\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28655646\"\u003eVitamin D deficiency impairs testicular development and spermatogenesis in mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28578315\"\u003eLysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis.\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28496042\"\u003eHigh sensitivity of testicular function to titanium nanoparticles\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29058779\"\u003eThe Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29187457\"\u003eTransdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28687593\"\u003eEffect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29525625\"\u003ePrenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/doi.org\/10.1093\/toxsci\/kfy167\"\u003eImpact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29403355\"\u003eImpact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/ediss.sub.uni-hamburg.de\/volltexte\/2018\/8921\/\"\u003eA Pex13 knockout in germ cells induces a spermatogenic arrest\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0753332218326428\"\u003eCrocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"http:\/\/aammt.tmmu.edu.cn\/Upload\/rhtml\/201801114.htm\"\u003e基因敲除损伤小鼠睾丸睾酮产生与生精功能\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e\u003ca href=\"https:\/\/www.spandidos-publications.com\/10.3892\/etm.2019.7352\"\u003eTanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary\u003c\/a\u003e\u003cp\u003e \u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48696686280804,"sku":"CEA441Mu","price":922.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_d9ad4829-19cd-4632-8603-4f3fd4eceec6.jpg?v=1783134105","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/lh-elisa-kit-for-mouse","provider":"GeneBio ","version":"1.0","type":"link"}