{"product_id":"human-nkx2-5-nk2-homeobox-5-elisa-kit","title":"Human NKX2-5(NK2 Homeobox 5) ELISA Kit","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:96T\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlternative Names\u003c\/b\u003e:Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; CSX; NKX2.5; NKX2E\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Type\u003c\/b\u003e:Sandwich\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSensitivity\u003c\/b\u003e:21.4 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStandard\u003c\/b\u003e:5000 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection range\u003c\/b\u003e:78.13-5000 pg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSample type\u003c\/b\u003e:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay length\u003c\/b\u003e:3.5h\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Area\u003c\/b\u003e:Signal transduction\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest principle\u003c\/b\u003e:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX2-5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX2-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX2-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX2-5 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniProt ID\u003c\/b\u003e:P52952\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48644873027684,"sku":"ELK0416","price":655.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_1cfae248-8ada-482f-952d-90125c57790c.jpg?v=1782659637","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/human-nkx2-5-nk2-homeobox-5-elisa-kit","provider":"GeneBio ","version":"1.0","type":"link"}