{"product_id":"hiscript-iv-rt-supermix-for-qpcr-gdna-wiper","title":"HiScript IV RT SuperMix for qPCR (+gDNA wiper)","description":"\u003cp\u003e\u003cstrong\u003eSize: 100 rxns \u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"tabs6 el-tabs el-tabs--top\"\u003e\n\u003cdiv class=\"el-tabs__content\"\u003e\n\u003cdiv class=\"el-tab-pane\" aria-labelledby=\"tab-aba8736c-1392-424d-93d3-7590b8b765b9\" id=\"pane-aba8736c-1392-424d-93d3-7590b8b765b9\" role=\"tabpanel\"\u003e\n\u003cdiv class=\"article-box\" id=\"contentHtml\"\u003e\n\u003cdiv\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Description  \u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eHiScript IV RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper), including a new generation of HiScript IV Reverse Transcriptase and Buffer optimized for reverse transcription. This kit further improves the synthesis efficiency of cDNA, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates. 5 × gDNA wiper Mix can completely remove the genomic contaimination in the RNA template, so there is no need to design intron-spanning qPCR primers. 4 × HiScript IV qRT SuperMix contains all the components required for the reverse transcription, just add template RNA and RNase-free ddH\u003csub\u003e2\u003c\/sub\u003eO to start the reaction.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003e Features\u003c\/strong\u003e  \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSuperior reverse transcription efficiency: Achieve qPCR detection results with smaller \u003c\/span\u003e\u003cspan\u003eC\u003csub\u003eT\u003c\/sub\u003e\u003c\/span\u003e\u003cspan\u003e values. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGreat gDNA elimination efficiency: Thoroughly remove 500 ng of gDNA to ensure the accuracy of qPCR detection. \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eExcellent template compatibility: Efficient reverse transcription of low-input, low-expression or degraded RNA templates.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e Components  \u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/a1f9b8a9a3f74e5498c8e68196135a2f\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003cstrong\u003eStorage  \u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eStore at -30 ~ -15°C and transport at ≤0°C.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e Performance  \u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFaster Reaction\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"185\" width=\"967\" alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/1d5d6f73c623439ea3a0612682e7854c\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eSuperior RT Efficiency\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e100 ng RNA from animals, plants and microbes were reverse transcribed by Vazyme #R423 and similar RT products from Supplier A,  Supplier B and Supplier C, following their respective manuals. The RT efficiency of these reagents was evaluated by qPCR amplification of obtained cDNA. It can be seen from the figure, △CT between Vazyme #R423 and Supplier C is within 0.5, indicating a  similar efficiency of them; △C\u003csub\u003eT\u003c\/sub\u003e between Vazyme #R423 and Suppler A\/Supplier B are more than 0.5, indicating a better efficiency of Vazyme #R423.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"438\" width=\"843\" alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/617833e697e44ff5a84ae8ed3b4be33a\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eG\u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003ereat gDNA Elimination Efficiency\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e500 ng gDNA from 293T cells were eliminated by Vazyme #R423 and similar RT products from Supplier A, Supplier B and  Supplier C. The digested gDNA were amplified by qPCR to evaluate the elimination efficiency of these products. The results  show that the gDNA elimination efficiency of Vazyme #R423 is consistent with that of Supplier A and Supplier B, and significantly  better than that of Supplier C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"311\" width=\"981\" alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/ef27d6cd477542029966fbf0d5e22ff3\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eExcellent Template Compatibility\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e100 ng human FFPE samples and degraded 293T cells (Rin=1) were reverse transcribed by Vazyme #R423 and similar RT  products from Supplier A, Supplier B and Supplier C. The template compatibility of these reagents was evaluated by qPCR amplification of obtained cDNA. The results show that △CT between Vazyme #R423 and Suppliers are more than 0.5 in most genes, indicating a better template compatibility of Vazyme #R423.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg height=\"403\" width=\"921\" alt=\"HiScript IV RT SuperMix for qPCR (+gDNA wiper)\" src=\"https:\/\/vazyme-singapore-website-prod.s3.ap-southeast-1.amazonaws.com\/2259dab1b2b44563a987a4895bfef55c\"\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":47172647878756,"sku":"R423-01","price":505.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Screenshot_136.png?v=1761319464","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/hiscript-iv-rt-supermix-for-qpcr-gdna-wiper","provider":"GeneBio ","version":"1.0","type":"link"}