{"product_id":"gb-ruler-unstained-protein-ruler","title":"GB-Ruler™ Unstained Protein Ruler","description":"\u003cspan data-mce-fragment=\"1\"\u003eThis \u003c\/span\u003eGB-Ruler™ \u003cspan data-mce-fragment=\"1\"\u003eUnstained Protein Ladder is a mixture of \u003c\/span\u003e\u003cu data-mce-fragment=\"1\"\u003e12 unstained recombinant proteins\u003c\/u\u003e\u003cspan data-mce-fragment=\"1\"\u003e covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands.  The This GB-Ruler™ Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.\u003c\/span\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure Caption\u003c\/strong\u003e-3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eStorage Buffer : \u003c\/strong\u003e\u003cbr\u003e20 mM Tris-phosphate(pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v\/v) Glycerol.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eQuality Control : \u003c\/strong\u003e\u003cbr\u003eUnder suggested conditions, Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eStorage :\u003c\/strong\u003e\u003cbr\u003eStable for up to 2 weeks at 25°C.\u003cbr\u003eStable for up to 3 months at 4°C.\u003cbr\u003eStable for up to 24 months at  -20°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAll products are for research use only.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eCaution :\u003c\/strong\u003e\u003cbr\u003eNot intended for human or animal diagnostic or therapeutic uses.\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"500uL","offer_id":39771533967460,"sku":"UPR01-01","price":47.95,"currency_code":"EUR","in_stock":true},{"title":"1 ml","offer_id":39771534000228,"sku":"UPR01-02","price":79.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_781bc6d7-8466-44c4-84e1-e2af15eaddd7.jpg?v=1671573853","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/gb-ruler-unstained-protein-ruler","provider":"GeneBio ","version":"1.0","type":"link"}