{"product_id":"anti-hsp60-hspd1-antibody-monoclonal-lk1","title":"Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100 μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eForm\u003c\/b\u003e: Lyophilized\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Chicken,Human,Mouse,Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e: IHC,WB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication Details\u003c\/b\u003e: Western blot, 2-4μg\/ml, Human, chicken, rat \u003cbr\u003e\nImmunohistochemistry (Paraffin-embedded Section), 4-8μg\/ml, Human, chicken, mouse, rat\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Name\u003c\/b\u003e: HSPD1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: No cross reactivity with other proteins.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e: Recombinant human heat shock protein 60 (HSP60)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e: Monoclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eContents\u003c\/b\u003e: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.\n*This antibody is supplied in a stabilized formulation. \u003cbr\u003e\nCompatibility with conjugation reactions depends on the chemistry of the conjugation method used. \u003cbr\u003e\nFor conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.\u003cbr\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification\u003c\/b\u003e: Ascites\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Add 1ml of PBS buffer will yield a concentration of 100ug\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReference\u003c\/b\u003e: 1. Cheng, M. Y.; Hartl, F.-U.; Martin, J.; Pollock, R. A.; Kalousek, F.; Neupert, W.; Hallberg, E. M.; Hallberg, R. L.; Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989.\n2. Hansen, J. J.; Bross, P.; Westergaard, M.; Nielsen, M. N.; Eiberg, H.; Borglum, A. D.; Mogensen, J.; Kristiansen, K.; Bolund, L.; Gregersen, N. : Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a biional promoter. Hum. Genet. 112: 71-77, 2003. Note: Erratum: Hum. Genet. 112: 436 only, 2003.\n3. Zanin-Zhorov, A.; Cahalon, L.; Tal, G.; Margalit, R.; Lider, O.; Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006.\n\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUniprot ID\u003c\/b\u003e: P63039\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: Adding 1 ml of PBS buffer will yield a concentration of 100 μg\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugate\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eCross Reactivity\u003c\/b\u003e: No cross-reactivity with other proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG1\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePhospho_site\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone Number\u003c\/b\u003e: Clone: LK1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObserved Molecular Weight\u003c\/b\u003e: 65 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated Molecular Weight\u003c\/b\u003e: 60955 MW\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID\u003c\/b\u003e: Human(3329), Mouse(15510), Rat(63868)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eProtein Name\u003c\/b\u003e: 60 kDa heat shock protein, mitochondrial\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene Full Name\u003c\/b\u003e: heat shock protein family D (Hsp60) member 1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms\u003c\/b\u003e: HSP 60; HSP60; HSP65; HSPD1\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48788058112100,"sku":"MA1049","price":506.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_69103a61-955b-4b16-b8e0-7d5eceb273b0.jpg?v=1784407094","url":"https:\/\/www.genebiosystems.com\/en-fr\/products\/anti-hsp60-hspd1-antibody-monoclonal-lk1","provider":"GeneBio ","version":"1.0","type":"link"}