{"title":"RNA Isolation: Cells and Tissues","description":"\u003cp\u003eIsolating all RNA molecules from a sample allows for messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA) to be extracted in an efficient and reproducible way while minimizing degradation and contamination. Applications include gene expression analyses, transcriptome profiling, functional genomics, microarray analyses, non-coding RNA studies, disease research, and quality control measures.\u003c\/p\u003e\n\u003cp\u003eOur streamlined total RNA isolation\/extraction kit is specialized for RNA isolation from cells, tissues, and yeast samples. Learn more about our easy-to-follow protocol with high-quality results:\u003c\/p\u003e","products":[{"product_id":"on-column-dnase-digestion-kit","title":"On-Column DNase Digestion Kit","description":"\u003cp\u003e\u003cstrong\u003e50rxns\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eDNase Digestion Kit (On Spin Columns) is for removing DNA on spin columns used during RNA isolation procedures. Although these procedures are capable of removing most DNA, small amount of DNA remains. For demanding applications where all DNA must be removed, on-column digestion can be performed. Same as Qiagen RNase free DNase set (Cat# 79254*).\u003c\/p\u003e\n\u003cp\u003eSee protocol details for RNI02 in manual:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/RNK3401_RNI02_Protocol_ON_Column_DNase_Digestion-_GBS_April_2025.pdf?v=1743602031\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e*Note: this catalog number is that of a product of Qiagen.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766549659748,"sku":"RNI02","price":108.63,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_194ad8f7-7c81-4ae4-af8a-50100cafdca9.jpg?v=1757609201"},{"product_id":"hipure-total-rna-mini-kit","title":"HiPure Total RNA Mini Kit (RNA Isolation\/Extraction Kit)","description":"\u003cdiv\u003eIsolation \/ extraction of total RNA from a large number of mammalian tissues and cells and yeast. \u003cbr\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003eProvides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA.\u003c\/li\u003e\n\u003cli\u003eNo need for phenol\/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated\u003c\/li\u003e\n\u003cli\u003eApplications: RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul class=\"list-paddingleft-2\"\u003e\n\u003cli\u003e\u003cspan\u003eHigh quality - high purity total RNA can be directly used in various sensitive downstream applications\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eFast - several samples can be extracted in 20 minutes by column method\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSafe - no phenol chloroform extraction required\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSensitive - RNA can be recovered at the level of picogram\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eStorage\/Stability: store kit dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.\u003c\/p\u003e\n\u003cp\u003eSee protocol details in manual:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R4011_HiPure_Total_RNA_Kit-GBS-50_and_250_preps.pdf?v=1700842592\" title=\"HiPUre Total RNA Kit\"\u003e\u003cimg height=\"60\" width=\"213\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eKit Contents\u003c\/p\u003e\n\u003ctable width=\"250\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e\u003cstrong\u003eProduct \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eR401102\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eR401103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003ePreparation Times\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 53px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 53px;\"\u003e\n\u003cp\u003eHiPure RNA Mini Columns\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 53px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 53px;\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e2 mL Collection Tubes\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e250\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eRTL Lysis Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e40 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e200 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eRNA Binding Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e15 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e75 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003eBuffer RW1\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e40 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e200 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e\n\u003cp\u003eBuffer RW2*\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e20 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px; height: 18px;\"\u003e2 x 50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 458.667px;\"\u003eRNase Free Water\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px;\"\u003e6 mL\u003c\/td\u003e\n\u003ctd style=\"width: 458.667px;\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eCitations:\u003c\/p\u003e\n\u003cp\u003eLouis L, Ramzan F, Martin DD, Olaitan AO.0.Reversion of heme-dependent metronidazole resistance in Clostridioides difficile. Microbiol Spectr0:e01787-25.https:\/\/doi.org\/10.1128\/spectrum.01787-25\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Preps","offer_id":39766553100388,"sku":"R401102","price":237.5,"currency_code":"CAD","in_stock":true},{"title":"250 Preps","offer_id":39766553133156,"sku":"R401103","price":745.92,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/20250328_151443.jpg?v=1743595623"},{"product_id":"trazol-rna-reagent","title":"TRA-zol RNA Isolation Reagent","description":"\u003cp\u003eTRA-zol Reagent is an improved version of the popular single-step total RNA isolation reagent developed by Chomczynski and Sacchi (1987). The RNA isolation (extraction) method based on this single reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.\u003c\/p\u003e\n\u003cp\u003eTRA-zol Reagent performs well with easily scalable small or large amounts of tissue or cells, and many samples can be simultaneously extracted.\u003c\/p\u003e\n\u003cp\u003eThis product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein upon homogenization or lysis of tissue sample. After adding chloroform or 1-bromo-3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. One milliliter of TRAzol is sufficient to isolate RNA, DNA, and protein from 50-100 mg of tissue, 5-10 million cells, or 10 cm² of culture dish surface for cells grown in monolayer.\u003c\/p\u003e\n\u003cp\u003eThis is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.\u003c\/p\u003e\n\u003cp\u003eThe RNA extraction reagent has been widely used for isolating RNA, similar to the leading RNA Isolation Reagents. Resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures and Benefits:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e• Easily scalable RNA isolation • Works with many sources: human, plant, yeast, bacterial, or viral • Better yields than traditional guanidine thiocyanate\/cesium chloride methods • Cost-effective compared to other similar single-reagent RNA isolation reagents\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Trazol_Magzol_Reagent_User_Manual.pdf?v=1688853244\" title=\"Protocol for Trazol Magzol\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"221\" height=\"62\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReference\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eChomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal. Biochem. 162: 156-159. 1987.\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"Gene Bio Systems","offers":[{"title":"200mL","offer_id":39766558703716,"sku":"R1022b","price":579.4,"currency_code":"CAD","in_stock":true},{"title":"400mL","offer_id":42713521029220,"sku":"R1022c","price":966.72,"currency_code":"CAD","in_stock":true},{"title":"3 x 400mL","offer_id":42713521061988,"sku":"R1022d","price":1305.64,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/TRAzol_20250314_105917.jpg?v=1742485128"},{"product_id":"hipure-universal-rna-kit","title":"HiPure Universal RNA Kit","description":"\u003cdiv\u003e\n\u003ch3\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eFor isolation (extraction) of total RNA from a large number of sample types, including cultured cells, tissues, plants and microbial samples. \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003ePhenol\/guanidine-based lysis and silica membrane purification of total RNA\u003c\/li\u003e\n\u003cli\u003eMagZol Reagent is a monophasic solution of phenol and guanidine thiocyanate, facilitates lysis of samples and inhibits RNases\u003c\/li\u003e\n\u003cli\u003eHigh lysis efficiency of reagent and subsequent removal of contaminants by organic phase extraction enables up to 200ug total purified RNA from less than 100mg animal plant, fungal samples, bacterial cells and other samples\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003ca style=\"font-size: 0.875rem;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R4130_HiPure_Universal_RNA_Kit_-_GBS.pdf?v=1700842764\"\u003e\u003cimg height=\"60\" width=\"213\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 preps","offer_id":41365767815268,"sku":"R413002","price":237.51,"currency_code":"CAD","in_stock":true},{"title":"250 preps","offer_id":41365767848036,"sku":"R413003","price":1065.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/Screenshot2022-12-30095750.jpg?v=1672412858"},{"product_id":"rna-safer-reagent","title":"RNA Safer Reagent","description":"\u003ch1 data-mce-fragment=\"1\"\u003eIntroduction\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRNA Safer Reagent is a safe tissue RNA protective agent. It can quickly penetrate into the cell, inactivate nuclease and protect RNA from degradation. Biological samples (tissues and cells) can be stored at room temperature (25°C) for one week, 4°C for one month, or -20°C \/ -80°C for long time by simply soaking in the reagent. RNA Safer Reagent is suitable for preserving various fresh biological samples, such as animal tissues, plant tissues, cultured cells, etc. Samples protected by RNA Safer Reagent can be more convenient for transportation and storage.\u003c\/p\u003e\n\u003ch1 data-mce-fragment=\"1\"\u003eDetails\u003c\/h1\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eKit Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eContents\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eP8310\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\"\u003eRNA Safer Reagent\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\"\u003e500 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRNA Safer Reagent can be stored at room temperature for 18 months. If crystal precipitation occurs during storage, redissolve it in 55°C water bath.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P8310_RNASafer_Reagent_-GBS.pdf?v=1700842489\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"213\" height=\"60\" data-mce-selected=\"1\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"100 ml","offer_id":41493369520228,"sku":"P8310-01","price":240.71,"currency_code":"CAD","in_stock":true},{"title":"500 ml","offer_id":41493369552996,"sku":"P8310-02","price":603.59,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/Screenshot_45.png?v=1675800226"},{"product_id":"hipure-total-rna-plus-kit","title":"HiPure Total RNA Plus Kit","description":"\u003ch2\u003eIntroduction\u003c\/h2\u003e\n\u003cp\u003eThis kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is \u003cstrong\u003eno need for phenol\/chloroform extractions\u003c\/strong\u003e and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.\u003c\/p\u003e\n\u003ch2\u003ePrinciple\u003c\/h2\u003e\n\u003cp\u003eThe Kit isolates total RNA from\u003cstrong\u003e up to 10\u003csup\u003e7\u003c\/sup\u003e cells or 20 mg tissue\u003c\/strong\u003e. A short workflow enables RNA isolation with genomic DNA removal in less than 25 mins. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAdvantages\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eEfficient removal of DNA \u003c\/strong\u003e- unique genomic DNA removal column \u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003ewithout\u003c\/span\u003e DNase treatment\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh quality\u003c\/strong\u003e - high purity total RNA can be directly used in various sensitive downstream applications\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFast\u003c\/strong\u003e - several samples can be extracted in 25 minutes by column method\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSafe \u003c\/strong\u003e- \u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003eno phenol chloroform extraction\u003c\/span\u003e\u003c\/strong\u003e required\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitive \u003c\/strong\u003e- RNA can be recovered at the level of PG\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eDetails\u003cstrong\u003e\u003c\/strong\u003e\n\u003c\/h3\u003e\n\u003cp\u003eSee protocol details in manual:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R4111_GBS-Manual_HiPure-Total-RNA-Plus_March-2025.pdf?v=1741196371\" title=\"HiPUre Total RNA Kit\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg height=\"60\" width=\"213\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R4011_HiPure_Total_RNA_Kit-GBS-50_and_250_preps.pdf?v=1700842592\" title=\"HiPUre Total RNA Kit\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFeatures\u003c\/td\u003e\n\u003ctd\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Functions\u003c\/td\u003e\n\u003ctd\u003eIsolation total RNA from 20mg tissue, 150mg plant, 5 x 10\u003csup\u003e6\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003ecell using two columns (gDNA removed column)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eRT-PCR, qRT-PCR, Northern hybridization, second generation sequencing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification method\u003c\/td\u003e\n\u003ctd\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification technology\u003c\/td\u003e\n\u003ctd\u003eSilica technology, DNA filtration technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcess method\u003c\/td\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eAnimal soft tissue, cultured cells, lymphocytes, simple plant tissue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cstrong\u003eSample amount\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eCells:\u003c\/strong\u003e≤1 x 10\u003csup\u003e7\u003c\/sup\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eAnimal tissue sample: \u003c\/strong\u003e1-20 mg\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003ePlant tissue:\u003c\/strong\u003e 50-150 mg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYield\u003c\/td\u003e\n\u003ctd\u003e2-100μg\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume\u003c\/td\u003e\n\u003ctd\u003eVariable to 50μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTime per run\u003c\/td\u003e\n\u003ctd\u003e≤25 minutes（1-24 samples）\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd\u003e800µl\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd\u003e100µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 310.031px;\"\u003eContents\u003c\/td\u003e\n\u003ctd style=\"width: 103.719px;\"\u003eR411102\u003c\/td\u003e\n\u003ctd style=\"width: 116.917px;\"\u003eD411103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 310.031px;\"\u003ePurification Times\u003c\/td\u003e\n\u003ctd style=\"width: 103.719px;\"\u003e50 Preps\u003c\/td\u003e\n\u003ctd style=\"width: 116.917px;\"\u003e250 Preps\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 310.031px;\"\u003eHiPure DNA Mini Columns\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 103.719px;\"\u003e50\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 116.917px;\"\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 310.031px;\"\u003eHiPure RNA Mini Columns\u003c\/td\u003e\n\u003ctd style=\"width: 103.719px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"width: 116.917px;\"\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 310.031px;\"\u003e2ml Collection Tubes\u003c\/td\u003e\n\u003ctd style=\"width: 103.719px;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"width: 116.917px;\"\u003e2 x\u003cspan\u003e \u003c\/span\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 310.031px;\"\u003eBuffer RLC\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 103.719px;\"\u003e40 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 116.917px;\"\u003e200 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 310.031px;\"\u003eBuffer RW1\u003c\/td\u003e\n\u003ctd style=\"width: 103.719px;\"\u003e40 ml\u003c\/td\u003e\n\u003ctd style=\"width: 116.917px;\"\u003e200 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 310.031px;\"\u003eBuffer RW2*\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 103.719px;\"\u003e\n\u003cspan\u003e20 \u003c\/span\u003eml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 116.917px;\"\u003e2 x 50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 310.031px;\"\u003eRNase Free Water\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 103.719px;\"\u003e10 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 116.917px;\"\u003e30 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage and Stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eHiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 rxn","offer_id":41767079346276,"sku":"R411102","price":296.35,"currency_code":"CAD","in_stock":true},{"title":"250 rxn","offer_id":41767079379044,"sku":"R411103","price":1329.35,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R411102_kit_image.jpg?v=1742213952"},{"product_id":"easypure-rna-kit","title":"EasyPure® RNA Kit","description":"\u003ch2\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eProduct Details\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eElevate your RNA isolation protocols with the EasyPure® RNA Kit, designed to deliver high-quality RNA from diverse biological samples, including \u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003eanimal cells, animal tissues, bacteria, and yeast\u003c\/strong\u003e\u003c\/span\u003e. This advanced kit employs a column-based method that ensures rapid and efficient RNA extraction. The process begins with enzymatic lysis of cells and tissues, followed by DNase I treatment to eliminate DNA contamination. RNA is then selectively bound to a silica membrane, washed, and eluted in its pure form, free from protein contaminants.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cb data-olk-copy-source=\"MessageBody\"\u003eContains DNase I\u003c\/b\u003e\u003c\/span\u003e\u003cb\u003e \u003c\/b\u003e\u003cspan\u003ewhich\u003c\/span\u003e\u003cb\u003e \u003c\/b\u003eremoves contaminating DNA from RNA samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSafe and Non-Toxic:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003eNo need\u003c\/strong\u003e\u003c\/span\u003e for \u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003ephenol and chloroform.\u003c\/span\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Yield and Purity:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eDelivers RNA suitable for RT-PCR, qRT-PCR, and Northern blotting.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVersatile:\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003eEffective for \u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003eanimal cells, tissues, bacteria, and yeast\u003c\/strong\u003e\u003c\/span\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eSee protocol details in manual:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/ER101_EasyPure-RNA-Kit_GBS-Manual_Feb-2025.pdf?v=1740489252\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg height=\"64\" width=\"228\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/ER111_EasyPure-Fast-RNA-Kit_GBS-Manual_Feb-2025.pdf?v=1740489252\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/EasyPure_Universal_Plant_Total_RNA_Kit_ER302-GBS-Manual_Jan_2025.pdf?v=1736365507\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2 class=\"product-content-sub-title\"\u003eSample requirements\u003c\/h2\u003e\n\u003ctable style=\"height: 122px; width: 58.5455%;\" width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px; width: 37.8947%;\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 27.273%;\"\u003eAmount\u003c\/td\u003e\n\u003ctd style=\"width: 33.6744%;\"\u003eVolume of BB4\/\u003cspan\u003eβ-ME\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px; width: 37.8947%;\"\u003e\n\u003cp\u003eAnimal cells\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 27.273%;\"\u003e\n\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e≤5x10^6\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 33.6744%;\"\u003e0.3-0.6 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"height: 19.5938px; width: 37.8947%;\"\u003eAnimal tissues\u003c\/td\u003e\n\u003ctd style=\"width: 27.273%;\"\u003e\n\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e≤20 mg\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 33.6744%;\"\u003e0.3-0.6 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 37.8947%;\"\u003eBacterial Cells\u003c\/td\u003e\n\u003ctd style=\"width: 27.273%;\"\u003e\n\u003cspan\u003e \u003c\/span\u003e\u003cstrong\u003e≤1x10^9\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 33.6744%;\"\u003e0.35 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e*BB4 with β-mercaptoethanol; add 10 μl of β-mercaptoethanol per 1ml of BB4, the solution must be freshly prepared just before use.\u003c\/p\u003e\n\u003ch2 class=\"product-content-sub-title\"\u003eKit Content\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eNot provided in kit:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eβ-mercaptoethanol\u003c\/li\u003e\n\u003cli\u003e96%-100% ethanol\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ctable border=\"1\" width=\"333\" height=\"311\" style=\"width: 276px;\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd style=\"width: 171.646px;\"\u003e\n\u003cp\u003eComponent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 90.3542px;\"\u003e\n\u003cp\u003eER101-01(50 rxns)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 171.646px;\"\u003eBinding buffer 4 (BB4)\u003c\/td\u003e\n\u003ctd style=\"width: 90.3542px;\"\u003e40 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 171.646px;\"\u003eClean buffer 4 (CB4)\u003c\/td\u003e\n\u003ctd style=\"width: 90.3542px;\"\u003e60 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 171.646px;\"\u003eWash buffer 4 (WB4)\u003c\/td\u003e\n\u003ctd style=\"width: 90.3542px;\"\u003e12 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 171.646px;\"\u003eProteinase K (20 mg\/ml)\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 90.3542px;\"\u003e1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 171.646px;\"\u003eDNase I (3 units\/μl)\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 90.3542px;\"\u003e1500 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 171.646px;\"\u003eDNase I Reaction Buffer\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 90.3542px;\"\u003e4×1 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 171.646px;\"\u003eRNase-free Water\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 90.3542px;\"\u003e10 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 171.646px;\"\u003eRNase-free Tube (1.5 ml)\u003c\/td\u003e\n\u003ctd colspan=\"1\" rowspan=\"1\" style=\"width: 90.3542px;\"\u003e50 each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 171.646px;\"\u003eRNA Spin Columns with Collection Tubes \u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 90.3542px;\"\u003e50 each\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 rxns","offer_id":45343071141988,"sku":"ER101-01","price":278.88,"currency_code":"CAD","in_stock":true},{"title":"200 rxns","offer_id":47199488933988,"sku":"ER101-02","price":794.64,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/20250328_150833.jpg?v=1743595982"}],"url":"https:\/\/www.genebiosystems.com\/en-fr\/collections\/rna-isolation-cells-and-tissues.oembed","provider":"GeneBio ","version":"1.0","type":"link"}