{"product_id":"transdetect-r-double-luciferase-reporter-assay-kit","title":"TransDetect® Double-Luciferase Reporter Assay Kit","description":"\u003cp\u003e\u003cstrong\u003eProduct Details\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFirefly luciferase and\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eRenilla\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eluciferase can catalyze the oxidation of luciferin or coelenterazine to form oxyluciferin or coelenteramide, respectively, and produce bioluminescence in the process.\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTransDetect\u003c\/em\u003e® Double-Luciferase Reporter Assay Kit first uses luciferin as a substrate to detect the activity of the firefly luciferase reporter gene. Then, while quenching the fluorescence reaction, the activity of the\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eRenilla\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eluciferase reporter gene was detected by using coelenterazine as a substrate. It has the characteristics of rapid detection, high sensitivity, wide detection range, and no interference with the endogenous activity of cells.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003cbr\u003eThe kit stored at -20°C for one year. Cell Lysis Buffer can be stored at -20°C for one year. Prepared Luciferase Reaction Reagent should be stored in aliquots in dark at -20°C for one month or at -70°C for one year.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/FR201_Double-Luciferase_Reporter_Assay_Kit-GBS_1a8cdcb6-30c8-42fa-818b-81e48a541049.pdf?v=1763965952\" target=\"_blank\" rel=\"noopener\"\u003e\u003cspan\u003e\u003cimg height=\"53\" width=\"188\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping\u003c\/strong\u003e\u003cbr\u003eDry ice (-70℃)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Contents\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eFR201-01-V2 (50 rxns)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eFR201-02 -V2(200 rxns)\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLuciferase Reaction Buffer\u003c\/td\u003e\n\u003ctd\u003e5 ml\u003c\/td\u003e\n\u003ctd\u003e20 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLuciferase Reaction Substrate (Lyophilized)\u003c\/td\u003e\n\u003ctd\u003e1 vial\u003c\/td\u003e\n\u003ctd\u003e4 vials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLuciferase Reaction Buffer II\u003c\/td\u003e\n\u003ctd\u003e5 ml\u003c\/td\u003e\n\u003ctd\u003e20 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLuciferase Reaction Substrate II (50×)\u003c\/td\u003e\n\u003ctd\u003e100 μl\u003c\/td\u003e\n\u003ctd\u003e400 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCell Lysis Buffer (5×)\u003c\/td\u003e\n\u003ctd\u003e5 ml\u003c\/td\u003e\n\u003ctd\u003e20 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCitations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eZhou W , Yao J , Wang G , \u003cem\u003eet al.\u003c\/em\u003e PKCζ phosphorylates TRAF2 to protect against intestinal ischemia–reperfusion–induced injury[J]. Cell Death \u0026amp; Disease, 2017, 8(7):e2935.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eShan W , Gao L , Zeng W , \u003cem\u003eet al\u003c\/em\u003e. Activation of the SIRT1\/p66shc antiapoptosis pathway via carnosic acid-induced inhibition of miR-34a protects rats against nonalcoholic fatty liver disease[J]. Cell Death and Disease, 2015, 6(7):e1833.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eXiong Y , Shi L , Wang L , \u003cem\u003eet al\u003c\/em\u003e. Activation of sirtuin 1 by catalpol-induced down-regulation of microRNA-132 attenuates endoplasmic reticulum stress in colitis[J]. Pharmacological Research, 2017:S1043661817303237.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eWang M , Gao Y , Qu P , \u003cem\u003eet al. \u003c\/em\u003eSperm-borne miR-449b influences cleavage, epigenetic reprogramming and apoptosis of SCNT embryos in bovine[J]. Scientific Reports, 2017, 7(1):13403.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eYang Z , Wang X , Yang J , \u003cem\u003eet al.\u003c\/em\u003e Aberrant CpG Methylation Mediates Abnormal Transcription ofMAO-AInduced by Acute and Chronicl-3,4-Dihydroxyphenylalanine Administration in SH-SY5Y Neuronal Cells[J]. Neurotoxicity Research, 2017, 31(3):334-347.\u003c\/span\u003e\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"50 Rxns","offer_id":41955078635620,"sku":"FR201-01","price":106.95,"currency_code":"EUR","in_stock":true},{"title":"200 Rxns","offer_id":41955078668388,"sku":"FR201-02","price":359.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/FR201-01.jpg?v=1689867092","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/transdetect-r-double-luciferase-reporter-assay-kit","provider":"GeneBio ","version":"1.0","type":"link"}