{"product_id":"recombinant-mouse-smpd1-asm-protein-his-tag","title":"Recombinant Mouse SMPD1\/ASM Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: A-SMase;ASM;Zn-SMase;aSMase\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Enzyme\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q04519\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: Q04519\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Sphingomyelin  phosphodiesterase 1 (SMPD1) , also known as ASM ( acid sphingomyelinase ), is a  member of the acid sphingomyelinase family of enzymes. Three isoforms have been  identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while  Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform  1 contains one saposin B-type domain that likely interacts with sphingomyelin,  and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1  is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane  lipid ) into ceramide through the removal of phosphorylcholine. This generates  second messenger components that participate in signal transduction. Defects in  SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also  known as Niemann-Pick disease classical infantile form and Niemann-Pick disease  visceral form. Niemann-Pick disease is a clinically and genetically  heterogeneous recessive disorder. NPB has little if any neurologic involvement  and patients may survive into adulthood.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine(HNPPC). The specific activity is \u0026gt; 1, 500 pmoles\/min\/μg. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Leu 626\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 85 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 0.1% Tween20 \u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 66.3 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 63 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47986026545252,"sku":"PKSM040504","price":837.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_eb4fbaa5-11a3-4262-9efb-236063cf97ae.jpg?v=1772479987","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/recombinant-mouse-smpd1-asm-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}