{"product_id":"recombinant-human-usp7-hausp-protein-aa-208-560-his-gst-tag","title":"Recombinant Human USP7\/HAUSP Protein (aa 208-560, His \u0026 GST Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: HAUSP;TEF1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Microbiology;Cell biology;Cancer;epigenetics and nuclear signal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-His-GST\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: Q93009\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_003461.2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Ubiquitin  carboxyl-terminal hydrolase 7, also known as Ubiquitin thioesterase 7,  Herpesvirus-associated ubiquitin-specific protease,  Ubiquitin-specific-processing protease 7, USP7 and HAUSP, is a widely expressed protein  which belongs to thepeptidase C19 family. USP7 is a member of the family  of deubiquitinating enzymes. It is involved in the regulation of stress  response pathways, epigenetic silencing and the progress of infections by DNA  viruses. USP7 is a protein with a cysteine peptidase core, N- and C-terminal  domains required for protein-protein interactions. USP7 contributes to  epigenetic silencing of homeotic genes by Polycomb (Pc). USP7 cleaves ubiquitin  fusion protein substrates. It deubiquitinates TP53\/p53 and MDM2 and strongly  stabilizes TP53 even in the presence of excess MDM2. USP7 also induces  TP53-dependent cell growth repression and apoptosis. USP7 has key roles in the  p53 pathway whereby it stabilizes both p53 and MDM2. Herpes simplex virus type  1 (HSV-1) regulatory protein ICP0 stimulates lytic infection and the  reactivation of quiescent viral genomes. ICP0 interacts very strongly with  USP7. USP7-mediated stabilization of ICP0 is dominant over ICP0-induced degradation  of USP7 during productive HSV-1 infection. The biological significance of the  ICP0-USP7 interaction may be most pronounced in natural infection situations,  in which limited amounts of ICP0 are expressed.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Lys 208-Glu 560\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 98 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM EDTA, pH 8.0\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 68.8 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 60 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47972946706532,"sku":"PKSH031030","price":714.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_7c31eb83-f76d-4fbe-b4fb-02b38d9df263.jpg?v=1772201496","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/recombinant-human-usp7-hausp-protein-aa-208-560-his-gst-tag","provider":"GeneBio ","version":"1.0","type":"link"}