{"product_id":"recombinant-human-mapk12-protein-his-tag","title":"Recombinant Human MAPK12 protein (His tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e:This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e:12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e:Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e:Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e:MAPK12\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e:ERK-6;ERK3;ERK6;MAPK12;P38-gamma;P38GAMMA;PRKM12;SAPK-3;SAPK3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e:Signal Transduction;Cell biology;\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e:Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e:E.coli\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e:N-His \u0026amp; C-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e:P53778\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e:P53778\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:ERK3, also known as MAPK12 and p38-gamma, belongs to the\u0026amp;nbsp,protein kinase superfamily, CMGC Ser\/Thr protein kinase family and MAP kinase subfamily. ERK3 is highly expressed in skeletal muscle and heart.\u0026amp;nbsp,ERK3 is a serine\/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e:Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e:Asp 101-Leu 367\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e: \u0026gt; 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:Lyophilized from sterile PBS, pH 7.4.\u003cbr\u003eNormally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg\/mL. Concentration is measured by UV-Vis.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:\u0026lt; 10 EU\/mg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e:29.3 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e:40 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48644130242660,"sku":"PDEH101048","price":838.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_fb0bd82a-951e-4df1-90cc-84a07fe9c6b9.jpg?v=1782614292","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/recombinant-human-mapk12-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}