Recombinant Human Ephrin-A4/EFNA4 Protein (Fc & His Tag)

SKU: PKSH032392

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Ephrin-A4;EFNA4

Target Synonym: EFNA4;EPH-Related Receptor Tyrosine Kinase Ligand 4;EPLG4;Ephrin-A4;LERK-4;LERK4

Research Areas: Cardiovascular;Neuroscience;Developmental Biology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-Fc-His

UNIProt ID: P52798

Accession: P52798

Background: Ephrin-A4 is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence; which most likely corresponds to the receptor binding domain. Ephrin-A4 consists of approximately 125 amino acids and includes four invariant cysteines; It has been shown to bind EphA2; EphA3; EphA4; EphA5; EphA6; EphA7; and EphB1. Ephrin-A4 binds promiscuously Eph receptors residing on adjacent cells; leading to contact-dependent bidirectional signaling into neighboring cells. It may play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.

Concentration:

Activity: Immobilized Human EphA7-His(Cat: PKSH033689) at 2μg/ml(100 μl/well) can bind Human EFNA4-Fc-6His. The ED50 of Human EFNA4-Fc-6His is 1. 5190 ug/ml.

Sequence: Leu26-Gly171

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 44.3 kDa

ObservedMW: 45 kDa