{"product_id":"recombinant-human-b7-h3-c-6his","title":"Recombinant Human B7-H3 (C-6His)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:50ug\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDescription\u003c\/b\u003e:Recombinant Human B7 Homolog 3 is produced by our Mammalian expression system and the target gene encoding Leu29-Thr461 is expressed with a 6His tag at the C-terminus.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e:Q5ZPR3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eMolecular weight\u003c\/b\u003e:47.3 KDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApparent molecular weight\u003c\/b\u003e:65-90 KDa, reducing conditions\u003c\/p\u003e\u003cp\u003e\u003cb\u003eOther names\u003c\/b\u003e:CD276; B7H34Ig-B7-H3; B7-H3; B7 homolog 3; CD276 antigen; CD276 molecule; Costimulatory molecule\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage condition\u003c\/b\u003e:Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:Greater than 95% as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:Less than 0.1 ng\/µg (1 EU\/µg) as determined by LAL test.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBiological activity\u003c\/b\u003e:Immobilized Human B7-H3-His(Cat#CK62) at 2μg\/ml (100 μl\/well) can bind Anti-Human B7-H3 mAb(Cat#NC055 ) The ED50 of Anti-Human B7-H3 mAb(Cat#NC055 ) is 7.98 ng\/ml.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRedissolve\u003c\/b\u003e:Always centrifuge tubes before opening.Do not mix by vortex or pipetting.\nIt is not recommended to reconstitute to a concentration less than 100μg\/ml.\nDissolve the lyophilized protein in distilled water.\nPlease aliquot the reconstituted solution to minimize freeze-thaw cycles. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:Lyophilized protein should be stored at \u0026lt; -20°C, though stable at room temperature for 3 weeks.\nReconstituted protein solution can be stored at 4-7°C for 2-7 days.\nAliquots of reconstituted samples are stable at \u0026lt; -20°C for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDelivery condition\u003c\/b\u003e:The product is shipped at ambient temperature.\nUpon receipt, store it immediately at the temperature listed below.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:CD276, also known as B7-H3, is a member of the B7 superfamily with signature IgV and IgG regions in extracellular domains. It is a type I transmembrane protein and shares 20–27% amino acid identity with other B7 family members. B7-H3 is involved in the activation of T lymphocytes, and regulates murine bone formation. It is also reported that B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is expressed on T-cells, natural killer cells, and antigen presenting cells, as well as some non-immune cells, such as osteoblasts, fibroblasts, fibroblast-like synoviocytes and epithelial cells. High expression of B7-H3 in tumor vasculature also correlates with poor survival in patients, suggesting that it may play a role in tumor cell migration.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48647912587364,"sku":"EPT290","price":806.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_a4b446e8-59b6-494a-9423-d7f22d9bb28c.jpg?v=1782704751","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/recombinant-human-b7-h3-c-6his","provider":"GeneBio ","version":"1.0","type":"link"}