{"product_id":"recombinant-human-ago1-argonaute-1-eif2c1-protein-his-tag","title":"Recombinant Human AGO1\/Argonaute 1\/EIF2C1 Protein (His Tag)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e: 50μg\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at \u0026lt; -20℃ for 3 months.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e: This product is provided as lyophilized powder which is shipped with ice packs.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e: 12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e: Recombinant Proteins\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e: Others\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAbbreviation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Synonym\u003c\/b\u003e: EIF2C;EIF2C1;GERP95;Q99\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResearch Areas\u003c\/b\u003e: Cell biology;epigenetics and nuclear signal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExpression Host\u003c\/b\u003e: Baculovirus-Insect Cells\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFusion tag\u003c\/b\u003e: N-His\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUNIProt ID\u003c\/b\u003e: B2RAD8\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAccession\u003c\/b\u003e: NP_036331.1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Protein argonaute-1, also known as  eukaryotic translation initiation factor 2C 1, EIF2C1, and AGO1, is a member of the argonaute family and ago  subfamily. Protein argonaute-1 in humans is encoded by the EIF2C1 gene. This  gene is located on chromosome 1 in a cluster of closely related family members  including argonaute 3, and argonaute 4. This genomic region is frequently lost  in human cancers such as Wilms tumors, neuroblastoma, and carcinomas of the  breast, liver, and colon. The human EIF2C1 gene is ubiquitously expressed at  low to medium levels. Differential polyadenylation and splicing result in a  complex transcriptional pattern.EIF2C1 protein contains onePAZ  domain and onePiwi domain. It is required for RNA-mediated gene silencing  (RNAi) and transcriptional gene silencing (TGS) of promoter regions which are  complementary to bound short antigene RNAs (agRNAs). EIF2C1 binds to short RNAs  such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses  the translation of mRNAs which are complementary to them.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eActivity\u003c\/b\u003e: Not validated for activity\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSequence\u003c\/b\u003e: Met 1-Ala 857\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurity\u003c\/b\u003e:  \u0026gt; 97 % as determined by reducing SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% glycerol, 0.5 PMSF, 0.5mM EDTA, pH 8.0\u003cbr\u003eNormally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.\u003cbr\u003ePlease refer to the specific buffer information in the printed manual.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e: Please refer to the printed manual for detailed information.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eEndotoxin\u003c\/b\u003e:  \u0026lt; 1.0 EU per μg of the protein as determined by the LAL method.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e: 99.5 kDa\u003c\/p\u003e\u003cp\u003e\u003cb\u003eObservedMW\u003c\/b\u003e: 99.5 kDa\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":47973010669668,"sku":"PKSH031192","price":719.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_f7cc9420-4968-4c72-ab36-c65a5c89c6d4.jpg?v=1772202288","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/recombinant-human-ago1-argonaute-1-eif2c1-protein-his-tag","provider":"GeneBio ","version":"1.0","type":"link"}