{"product_id":"purified-rabbit-anti-plc-gamma-1-py1253-antibody","title":"Purified Rabbit anti-PLC gamma 1 (pY1253) Antibody","description":"\u003cp\u003e\u003cb\u003eSize 1\u003c\/b\u003e: 100 ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms \u003c\/b\u003e: PLC1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1; PLC-gamma-1\u003c\/p\u003e\u003cp\u003e\u003cb\u003ehost\u003c\/b\u003e: Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）\u003c\/b\u003e: H, M, R, Mk\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e: WB, IF\/IC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Rabbit polyclonal antibody to PLC gamma 1 (pY1253)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: 1mg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PLC gamma 1. The exact sequence is proprietary.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWB description\u003c\/b\u003e: Western blot analysis of PLC gamma 1 (pY1253) expression in MCF7 EGF-treated (A), A431 (B), mouse brain (C), mouse liver (D), H9C2 EGF-treated (E) whole cell lysates.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIHC description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eIF\/ICC description\u003c\/b\u003e: Immunofluorescent analysis of PLC gamma 1 (pY1253) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIP description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFC description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eChIP description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification_Method\u003c\/b\u003e: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e: Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e: WB (1\/500 - 1\/1000), IF\/IC (1\/100 - 1\/500)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Human)\u003c\/b\u003e: 5335\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID (Mouse)\u003c\/b\u003e: 18803\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Rat)\u003c\/b\u003e: 25738\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Human)\u003c\/b\u003e: P19174\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Mouse)\u003c\/b\u003e: Q62077\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Rat)\u003c\/b\u003e: P10686\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at -20℃. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48738484256868,"sku":"DL90896A","price":620.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_f91dc6b5-4e70-4474-a5de-e9c29a599b4b.jpg?v=1783810399","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/purified-rabbit-anti-plc-gamma-1-py1253-antibody","provider":"GeneBio ","version":"1.0","type":"link"}