{"product_id":"purified-rabbit-anti-akt-pt308-antibody","title":"Purified Rabbit anti-AKT (pT308) Antibody","description":"\u003cp\u003e\u003cb\u003eSize 1\u003c\/b\u003e: 100 ul\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSynonyms \u003c\/b\u003e: AKT1; PKB; RAC; RAC-alpha serine\/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine\/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT3; PKBG; RAC-gamma serine\/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2\u003c\/p\u003e\u003cp\u003e\u003cb\u003ehost\u003c\/b\u003e: Rabbit\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）\u003c\/b\u003e: H, M, R, B, C, Z\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications\u003c\/b\u003e: WB, IF\/IC, IP\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e: Rabbit polyclonal antibody to AKT (pT308)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration\u003c\/b\u003e: 1mg\/mL\u003c\/p\u003e\u003cp\u003e\u003cb\u003eImmunogen\u003c\/b\u003e: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AKT. The exact sequence is proprietary.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eWB description\u003c\/b\u003e: Western blot analysis of AKT (pT308) expression in HeLa colchicine-treated (A), HL60 (B), NIH3T3 (C), SP2\/0 colchicine-treated (D), PC12 colchicine-treated (E) whole cell lysates.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIHC description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eIF\/ICC description\u003c\/b\u003e: Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIP description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eFC description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eChIP description\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification_Method\u003c\/b\u003e: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e: Polyclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e: \u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDilution\u003c\/b\u003e: WB (1\/500 - 1\/1000), IF\/IC (1\/100 - 1\/500), IP (1\/10 - 1\/100)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Human)\u003c\/b\u003e: 207; 208; 10000\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID (Mouse)\u003c\/b\u003e: 11651; 11652; 23797\u003c\/p\u003e\u003cp\u003e\u003cb\u003eGene ID(Rat)\u003c\/b\u003e: 24185; 25233; 29414\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Human)\u003c\/b\u003e: P31749; P31751; Q9Y243\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Mouse)\u003c\/b\u003e: P31750; Q60823; Q9WUA6\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSwissProt (Rat)\u003c\/b\u003e: P47196; P47197; Q63484\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: Store at -20℃. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48738677981284,"sku":"DL90032A","price":668.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_0b433997-3912-4dbe-823b-89e249b0f1f0.jpg?v=1783814777","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/purified-rabbit-anti-akt-pt308-antibody","provider":"GeneBio ","version":"1.0","type":"link"}