{"product_id":"one-step-tunel-flow-cytometry-apoptosis-kit-green-fitc","title":"One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100Assays\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e:Gel Packs\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e:12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_I\u003c\/b\u003e:Apoptosis and Cell Health Detection\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e:Cell Apoptosis\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e:TUNEL Assay Kits\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies reactivity\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUsage\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eKit Type\u003c\/b\u003e:Apoptosis\/DNA Fragmentation\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResult Type\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Instrument\u003c\/b\u003e:Flow Cytometer\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Sample Types\u003c\/b\u003e:Cell samples\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Time\u003c\/b\u003e:2.5-3 hours\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Type\u003c\/b\u003e:Fluorometric method;Flow cytometry\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest Principle\u003c\/b\u003e:When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBinding Capacity\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e:\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48665605701732,"sku":"E-CK-A420","price":710.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_5ff4dd1a-d596-4fca-82b0-18bdfb890cbf.jpg?v=1782866824","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/one-step-tunel-flow-cytometry-apoptosis-kit-green-fitc","provider":"GeneBio ","version":"1.0","type":"link"}