{"product_id":"mitochondrial-membrane-potential-assay-kit-with-jc-1","title":"Mitochondrial Membrane Potential Assay Kit (with JC-1)","description":"\u003cp\u003e\u003cb\u003eSize\u003c\/b\u003e:100Assays\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e:This product can be stored at 2~8°C\/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eShipping\u003c\/b\u003e:Gel Packs\u003c\/p\u003e\u003cp\u003e\u003cb\u003eExp date\u003c\/b\u003e:12 months\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_I\u003c\/b\u003e:Apoptosis and Cell Health Detection\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_II\u003c\/b\u003e:Cell Apoptosis\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCategory ID_III\u003c\/b\u003e:Mitochondrial Assay Kits\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConjugation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTarget Species\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecies reactivity\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eUsage\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eKit Type\u003c\/b\u003e:Apoptosis\/JC-1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eResult Type\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Instrument\u003c\/b\u003e:Flow Cytometer;Fluorescence Microscope\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Sample Types\u003c\/b\u003e:Cell samples\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAssay Time\u003c\/b\u003e:40min\u003c\/p\u003e\u003cp\u003e\u003cb\u003eDetection Type\u003c\/b\u003e:Fluorometric method;Flow cytometry\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBackground\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTest Principle\u003c\/b\u003e:The decrease of mitochondrial membrane potential is a marker event in the early stage of apoptosis. It occurs before the appearance of nuclear apoptotic features (chromatin condensation and DNA fragmentation). Once the mitochondrial membrane potential collapses, apoptosis is irreversible.\nJC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential ?Ψm. In normal cells, the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial matrix to form a polymer, which can produce red fluorescence. In the early stage of apoptosis, the mitochondrial membrane potential decreases, JC-1 can’t accumulate in the mitochondrial matrix. When JC-1 is a monomer, it can produce green fluorescence.\nThe decrease of cell membrane potential can be detected by the transition of JC-1 from red fluorescence to green fluorescence, and the transition of JC-1 fluorescence color can be used as an early detection indicator of cell apoptosis. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization.\nThe maximum excitation wavelength of JC-1 monomer is 514 nm and the maximum emission wavelength is 529 nm; the maximum excitation wavelength of JC-1 polymer is 585 nm and the maximum emission wavelength is 590 nm.\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBinding Capacity\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eBuffer\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReconstitution\u003c\/b\u003e:\u003c\/p\u003e\u003cp\u003e\u003cb\u003eCalculated MW\u003c\/b\u003e:\u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"Default Title","offer_id":48665604653156,"sku":"E-CK-A301","price":526.95,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/no_image_default_image-jpeg_5a8dc8d0-7a06-44d5-81e0-858a436bc274.jpg?v=1782866778","url":"https:\/\/www.genebiosystems.com\/en-de\/products\/mitochondrial-membrane-potential-assay-kit-with-jc-1","provider":"GeneBio ","version":"1.0","type":"link"}