HIF1a antibody , Cat. # FNab09940

SKU: FNab09940

Size:100ug

Purification:protein A+G purified

Form:liquid

Purity:?95% as determined by SDS-PAGE

Host:Mouse

Clonality:monoclonal

Clone ID:5H12

Isotype:IgG1

Storage:PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)

Background:Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).

Immunogen:hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)

Synonyms:BHLHE78, MOP1, PASD8

Observed MW:120 kDa

Uniprot ID:Q16665

Reactivity:Human

Tested Application:ELISA, IF, WB

Recommended dilution:WB: 1:1000-1:10000; IF: 1:50-1:200

Gene ID:3091

Research Area:Signal Transduction, Metabolism, Cardiovascular, Developmental biology, Neuroscience, Stem Cells