{"title":"New products","description":"","products":[{"product_id":"easyspin-plant-mirna-kit","title":"EASYspin Plant miRNA kit","description":"\u003cp\u003eEASYspin Plant miRNA kit\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Preps","offer_id":39752492974180,"sku":"RNK4001-01","price":395.05,"currency_code":"CAD","in_stock":true},{"title":"100 Preps","offer_id":39752493006948,"sku":"RNK4001-02","price":768.14,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/172.jpg?v=1643252325"},{"product_id":"gb-amp-2-x-pcr-master-mix-with-loading-dye","title":"GB-Amp™ 2X PCR Master mix (with loading dye)","description":"\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003ePCR Master Mix is a premixed solution containing all essential components and reagents required to conduct a PCR assay. It significantly simplifies the analysis of PCR products and the preparation of PCR samples. It is specified for\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cspan style=\"font-weight: 400;\"\u003erequiring high sensitivity, such as somatic mutations and microbial DNA detection. Generally, the master mix catalog includes thermostable Taq DNA polymerase recombinant, dNTPs, stabilizers, cofactor (MgCl2), and enhancers, all in a reaction buffer.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eApplications of GB-Amp™ 2X PCR Master Mix Products\u003c\/b\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eRoutine PCR\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003ePrimer dimers reduction\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eVarious primer-extension reactions, including labeling, signaling, and sequencing\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\n\u003cb\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch2\u003e\u003cb\u003eFeatures and Benefits of our Product\u003c\/b\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eIncredible amplification — The GB-Amp™ 2X PCR Master mix guarantees up to 6 kb amplification of PCR products.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\u003cspan style=\"font-weight: 400;\"\u003eIt is resistant to impurities.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cspan style=\"font-weight: 400;\"\u003eQuick and convenient — Our 2x master mixes are equipped with gel loading dyes and other additives, including Taq DNA polymers facilitating direct loading of the products and templates onto the agarose gels.\u003c\/span\u003e \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eThe GB-Amp™ 2X PCR Master mix is designed to make work easier, save time, and eliminate errors in proportioning and measurements. If you have been looking to streamline and improve your lab experiments, the state-of-the-art GB-Amp™ 2X PCR Master mix is a great option. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-weight: 400;\"\u003eDo you need to use both Taq, Pfu or any other proofreading polymerase for PCR? In this case, our 5min TA-Blunt-Zero Cloning Kit is the perfect choice. This is where we come in. We have your back, no matter the scope and size of your laboratory. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GB-Amp_2_x_PCR_Master_mix_with_loading_dye.pdf?v=1700752097\"\u003e\u003cspan style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cimg height=\"46\" width=\"164\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL","offer_id":39752493170788,"sku":"P3001","price":22.83,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":39752493203556,"sku":"P3002","price":98.09,"currency_code":"CAD","in_stock":true},{"title":"10mL","offer_id":39752493236324,"sku":"P3003","price":166.96,"currency_code":"CAD","in_stock":true},{"title":"50mL","offer_id":39752493269092,"sku":"P3004","price":728.95,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/2_2e0d719f-3c21-459e-be9c-3155512f5fe6.jpg?v=1751570904"},{"product_id":"gb-ruler-100bp-dna-ladder-plus","title":"GB-Ruler™ 100 bp DNA Ladder Plus","description":"\u003cdiv\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eGB-Ruler™ \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e100 bp Plus DNA Ladder is ideal for determining the size of double-stranded DNA from 100 to 3,000 base pairs. The ladder consists of 14 linear double-stranded fragments. The\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cu\u003e50\u003c\/u\u003e\u003c\/strong\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cu\u003e0bp\u003c\/u\u003e\u003c\/strong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eand\u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003e\u003cu\u003e1,200bp\u003c\/u\u003e\u003c\/strong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e \u003c\/span\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003efragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 500bp and 1,200bp are 40ng. The 500bp and 1,200bp fragments are 100ng. This ladder is pre-mixed with loading dye and is ready to use.\u003c\/span\u003e\u003cbr\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended Loading\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2-5 µl\/lane\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTypical Bands 100ng\/5µl\u003c\/p\u003e\n\u003cp\u003eOther Bands 40ng\/5µl\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended Electrophoresis Condition\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e5 µl\/lane, 8cm 1.7% Agarose Gel, 0.5×TBE, 5V\/cm, 1h.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eContents\u003c\/strong\u003e\u003cstrong\u003e(\u003c\/strong\u003e\u003cstrong\u003ebp\u003c\/strong\u003e\u003cstrong\u003e)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e100,200,300,400,\u003cstrong\u003e\u003cu\u003e500\u003c\/u\u003e\u003c\/strong\u003e,600,700,800,900,1,000,\u003cstrong\u003e\u003cu\u003e1,200\u003c\/u\u003e\u003c\/strong\u003e,1,500,2,000,3,000.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStable for 3 months at RT, for long-time storage, please store at -20 ̊C.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"347 ul (100 uses)","offer_id":39752493334628,"sku":"M1071","price":49.79,"currency_code":"CAD","in_stock":true},{"title":"1735 ul (500 uses)","offer_id":39752493367396,"sku":"M1072","price":198.13,"currency_code":"CAD","in_stock":true},{"title":"5205 ul (1500 uses)","offer_id":42713461031012,"sku":"M1073","price":544.32,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/100_bp_ladder_plus_image_2.jpg?v=1742212758"},{"product_id":"gb-ruler-1kb-dna-ladder","title":"GB-Ruler™ 1 kb DNA Ladder","description":"\u003cp\u003eThe quantity of DNA fragment or band is provided for you to estimate the concentration of your bands from the sample.\u003c\/p\u003e\n\u003cdiv class=\"title\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"title\"\u003e\u003cstrong\u003eFeatures and Specifications\u003c\/strong\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cbr\u003e\u003cstrong\u003eSize Range :\u003c\/strong\u003e 250 bp to 10 kb\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eNumber of bands:\u003c\/strong\u003e 13 bands\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eTotal Concentration:\u003c\/strong\u003e 100 μg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRecommended Volume:\u003c\/strong\u003e 5 μl per well\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eLoading buffer: \u003c\/strong\u003e10 mM Tris-HCl (pH 8), 10 mM EDTA, bromophenol blue and glycerol.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e 25°C for 6 months and -20°C for 24 months.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"347 ul (100 uses)","offer_id":39752493531236,"sku":"M1181","price":45.5,"currency_code":"CAD","in_stock":true},{"title":"1735 ul (500 uses)","offer_id":39752493564004,"sku":"M1182","price":210.31,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/179.jpg?v=1643252351"},{"product_id":"pfu-dna-polymerase","title":"Pfu DNA Polymerase","description":"\u003cp\u003ePfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.\u003c\/p\u003e\n\u003cp\u003eComponents: Pfu DNA polymerase (2.5 U\/ul or 5\u003cspan data-mce-fragment=\"1\"\u003e U\/ul-please see user manual\u003c\/span\u003e), 10x Pfu buffer\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e3'-5' exonuclease activity provides a low error rate\u003cbr\u003eOne of the most thermostable DNA polymerases known\u003cbr\u003eLack of extendase activity means no unwanted 3’ overhangs\u003cbr\u003eOptimal for blunt-ended PCR cloning\u003cbr\u003eOptimum temperature near 75°C\u003cbr\u003e95% active after 1hour incubation at 98°C\u003cbr\u003e\u003cbr\u003eApplications\u003cbr\u003eHigh-fidelity PCR and primer-extension reactions\u003cbr\u003ePCR cloning and blunt-ended amplification product generation\u003cbr\u003e\u003cbr\u003eUnit Definition\u003cbr\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.\u003cbr\u003e\u003cbr\u003eStore all components at –20°C\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/B600003_EN_P-UM-Pfu_DNA_Polymerase-GBS.pdf?v=1700584401\"\u003e\u003cimg height=\"42\" width=\"149\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"250 U","offer_id":39752493957220,"sku":"P1021","price":52.67,"currency_code":"CAD","in_stock":true},{"title":"1000 U","offer_id":39752493989988,"sku":"P1023","price":164.61,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_f893e40b-0727-4daa-a0e2-7769aa7f6e68.jpg?v=1706709482"},{"product_id":"ultrabright-led-transilluminator","title":"MAESTROGEN UltraBright LED Transilluminator","description":"\u003cdiv class=\"list_box\"\u003e\n\u003ch2 class=\"h3\"\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eTransilluminator \u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Transilluminators.pdf?v=1705611209\"\u003e\u003c\/a\u003e\n\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list_box\"\u003e\n\u003ch2 class=\"title\"\u003eFeatures\u003c\/h2\u003e\n\u003cp class=\"txt\"\u003e-Safe to use, as you are not working with UV\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Providing robust R\/G\/B LEDs for excitation\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Three wavelengths supported in a single transilluminator\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Delta LED configuration\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Providing a high degree of uniformity across entire viewing area\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eThis unit comes pre-installed with an Amber filter lid for the BLUE LED fluorescence. \u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e More information on the transilluminators can be found \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Transilluminator_Brochure.pdf?v=1680096633\"\u003ehere\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list_box\"\u003e\n\u003cdiv class=\"title\"\u003eSpecifications\u003c\/div\u003e\n\u003cdiv class=\"title\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e• Illumination type:\u003c\/strong\u003e LED\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e• Wavelength (nm):\u003c\/strong\u003e 470nm (Blue), 530nm (Green), 630nm (Red)\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e• Dimension (mm):\u003c\/strong\u003e 340L*280W*80H\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e• Viewing surface (mm):\u003c\/strong\u003e 160D*200W\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e• LED lamps arrangement:\u003c\/strong\u003e Delta matrix\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e• LED lifetime:\u003c\/strong\u003e 50,000 hrs\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e• Weight (Kg):\u003c\/strong\u003e 3.6\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003e• Power:\u003c\/strong\u003e 24V - 1.25A\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39752494088292,"sku":"GLB-17","price":1909.39,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Fig_3_GLB-17.png?v=1760913299"},{"product_id":"ultraslim-led-illuminator","title":"MAESTROGEN UltraSlim LED illuminator","description":"\u003ch2 class=\"h3\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eTransilluminator\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThis product comes with a color choice for the base unit: GSLB-01 (Black), or GSLB-01w (White )\u003c\/p\u003e\n\u003cdiv class=\"list_box\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Small size with only 3 cm high, THINNEST transilluminator available anywhere.\u003cbr\u003e\u003cbr\u003e• With the LED design, you can cut bands off agarose gel on your bench, without the UV safety goggles or face shields.\u003cbr\u003e\u003cbr\u003e• Light weight, you can share this box with your colleagues in the lab\u003cbr\u003e\u003cbr\u003e• LED design also means that your DNA sample is safe from UV damage, so are you face, hands and skins.\u003cbr\u003e\u003cbr\u003e• High detection sensitivity supporting detection of DNA bands with as low as 0.5 ng\u003cbr\u003e\u003cbr\u003e• High uniformity, \u0026lt;10% coefficient of variance (CV) across the main viewing area\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list_box\"\u003e\n\u003cdiv class=\"title\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"title\"\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Dimensions (cm): 21D x 21W x 3H\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Viewing surface (cm): 12D x 7W\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Sensitivity: as low as 0.5 ng\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Uniformity: \u0026lt; 10 % CV (coefficient of variance)\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• LED Wavelength (nm): 470\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Power: 24V - 1.25A\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e• Weight (kg): 2.3\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"Black","offer_id":43017028501604,"sku":"GSLB-01","price":1524.1,"currency_code":"CAD","in_stock":true},{"title":"White","offer_id":43017028534372,"sku":"GSLB-01w","price":1524.1,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/206.jpg?v=1643252421"},{"product_id":"slider-led-imager","title":"MAESTROGEN Slider LED Imager","description":"\u003cdiv class=\"title\"\u003e\u003cem\u003e\u003cspan data-olk-copy-source=\"MessageBody\"\u003eThis price is a signal that a quotation must be obtained before placing an order. Please reach out to us for a quote\u003c\/span\u003e\u003c\/em\u003e\u003c\/div\u003e\n\u003cdiv class=\"title\"\u003e\u003cstrong\u003e\u003cspan data-olk-copy-source=\"MessageBody\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"title\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/div\u003e\n\u003cp class=\"txt\"\u003eThe Slider imager is for gel viewing band curring and gel imaging, all in one.\u003c\/p\u003e\n\u003cp\u003eWe have also set a low price compared to similar products to help you make your acquisition of this unit.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eDark room for photography furnished by a brand-name camera.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eWiFi connectivity, so that you do not need to use the memory card all the time.\u003c\/p\u003e\n\u003cp\u003eThis means that you can transfer and print your imaging data on your computer using either the WiFi or the memory card, whichever is convenient to you.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cb\u003eThe \u003c\/b\u003eLCD monitor can be rotated to help you easily perform photography.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLight source: 470 nm.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39752494317668,"sku":"GSML-01","price":1.04,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/210.jpg?v=1643252429"},{"product_id":"slider-uv-imager","title":"MAESTROGEN Slider UV Imager","description":"\u003cdiv class=\"title\"\u003e\u003cem\u003e\u003cspan data-olk-copy-source=\"MessageBody\"\u003eThis price is a signal that a quotation must be obtained before placing an order. Please reach out to us for a quote\u003c\/span\u003e\u003c\/em\u003e\u003c\/div\u003e\n\u003cdiv class=\"title\"\u003e\u003cspan data-olk-copy-source=\"MessageBody\"\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv class=\"title\"\u003eFeatures\u003c\/div\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eMaestrogen provides a Slider imager for gel photography imaging and cutting.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003eEasy: \u003c\/strong\u003e\u003cbr\u003eGel photography imager and cutting at the same instrument\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003eAffordable: \u003c\/strong\u003e\u003cbr\u003eCompetitive price\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003eQuality: \u003c\/strong\u003e\u003cbr\u003eBranded digital camera, ~100 % dark room for photography\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003eFriendly: \u003c\/strong\u003e\u003cbr\u003eWifi interface, no need to take out the memory card frequently.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e**Transfer and print data directly with your smart device via WiFi function**\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e\u003cstrong\u003eConsiderate: \u003c\/strong\u003e\u003cbr\u003eRotatable LCD monitor for user easily perform photography.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eLight source from MaestroGen 302 nm UV transilluminator\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eSafe: Cutting gel with MaestroGen UltraSafe UV shield (99.99 % UV cut off)\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eEasy Powering: \u003cbr\u003eNO need to change and re-charge batteries on camera\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39752494350436,"sku":"GSMU-01","price":1.04,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/213.jpg?v=1643252438"},{"product_id":"high-resolution-hr-agarose","title":"High Resolution (HR) Agarose","description":"\u003cp\u003eYou can have resolution of DNA and RNA similar to that seen with polyacrylamide gels. \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"10 g","offer_id":39752494415972,"sku":"ELA-01-01","price":94.37,"currency_code":"CAD","in_stock":true},{"title":"25 g","offer_id":39752494448740,"sku":"ELA-01-02","price":219.48,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/221.jpg?v=1643252443"},{"product_id":"minicute-imager","title":"MAESTROGEN MiniCute™ Imager","description":"\u003cdiv class=\"title\"\u003e\u003cem\u003e\u003cspan data-olk-copy-source=\"MessageBody\"\u003eThis price is a signal that a quotation must be obtained before placing an order. Please reach out to us for a quote\u003c\/span\u003e\u003c\/em\u003e\u003c\/div\u003e\n\u003cdiv class=\"title\"\u003e\u003cem\u003e\u003cspan data-olk-copy-source=\"MessageBody\"\u003e\u003c\/span\u003e\u003c\/em\u003e\u003c\/div\u003e\n\u003cdiv class=\"title\"\u003eFeatures\u003c\/div\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eMiniCute imager is an easy gel image system , recommend to use with UltraSlim® LED Illuminator, UltraBright LED Transilluminator and UltraBright UV Transilluminator\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003eMiniCute imager, compact size, compatible with Maestrogen’s UV and blue LED transilluminators.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e • 100% fit to all Maestrogen's LED or UV illuminators\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e • Branded digital camera, 100% dark room for photography\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e • Light is convenient to move around (option)\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766537273444,"sku":"GMC-02","price":1.04,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/217.jpg?v=1643677144"},{"product_id":"plexprep-4-rna-dna-mirna-protein-extract-kit","title":"PlexPrep-4 RNA-DNA-miRNA-protein Extract Kit","description":"\u003cp\u003eThe Kit is used to extract and collect RNA,DNA,miRNA,protein separately from blood. Kits of 50 or 100 preps are available.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766537732196,"sku":"RNK4701-01","price":493.81,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766537764964,"sku":"RNK4701-02","price":932.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/222.jpg?v=1643677149"},{"product_id":"gb-amp-10-mm-each-dntp-mixture","title":"GB-Amp™ 10mM dNTP Mixture","description":"\u003cp\u003eA mixture of dNTPs with 10mM each of dATP, dCTP, dGTP, dTTP. RNAse, DNAse-free, high purity. \u0026gt;2 year Stablity at -20 C. Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GB-Amp_10_mM_each_dNTP_Mixture.docx?v=1700752043\"\u003e\u003cspan\u003e\u003cimg height=\"46\" width=\"164\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-selected=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"0.5 ml","offer_id":39766537896036,"sku":"PCN-01-01","price":30.72,"currency_code":"CAD","in_stock":true},{"title":"2 ml","offer_id":39766537928804,"sku":"PCN-01-02","price":96.57,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_6fcee860-b060-462d-a604-77d0f575d68a.jpg?v=1706882362"},{"product_id":"gb-amp-25-mm-each-dntp-mixture","title":"GB-Amp™ 25mM dNTP Mixture","description":"\u003cp\u003eA mixture of dNTPs with 25 mM each of dATP, dCTP, dGTP, dTTP. RNAse, DNAse-free, high purity. \u0026gt;2 year Stablity at -20 C. Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/dNTP_Mix-25_mM_each.docx?v=1700594064\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg height=\"42\" width=\"149\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-selected=\"1\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"0.5 ml","offer_id":39766538027108,"sku":"RNK2401-01","price":38.4,"currency_code":"CAD","in_stock":true},{"title":"2 ml","offer_id":39766538059876,"sku":"RNK2401-02","price":131.68,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_c707bca6-9ba3-4a58-9cfa-fa073a36e067.jpg?v=1706882153"},{"product_id":"gb-amp-100-mm-dutp","title":"GB-Amp™ 100mM dUTP","description":"\u003cp\u003e Can be used in polymerase and other enzyme catalyzed reactions, such as PCR, isothermal amplification and sequencing reactions.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"0.25 ml","offer_id":39766538289252,"sku":"PCN-01","price":54.87,"currency_code":"CAD","in_stock":true},{"title":"1 ml","offer_id":39766538322020,"sku":"PCN-02","price":197.52,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/226.jpg?v=1643677164"},{"product_id":"achievertm-dna-polymerase","title":"Achiever(TM) DNA Polymerase","description":"\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eAchiever(TM) DNA Polymerase allows amplification of the higher fidelity and longer templates than the single-enzyme formulations. It is also a better choice for amplifying complex template, such as GC-rich and secondary structure-harboring template. And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. In addition, Using Taq plus results in 3´-dA overhangs PCR products, which can be used in TA clone. \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eHigher fidelity: Its fidelity is four times higher than ordinary Taq polymerase.\u003cbr\u003eHigh yields: Suitable for amplifying complex template which is rich in GC or repeat sequence or that contains secondary structures.\u003cbr\u003eLong-range amplification by PCR. Large fragments as large as 20 kb for plasmid, phage DNA and 10 kb genomic DNA can be routinely amplified. \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eQuality Control\u003c\/strong\u003e \u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests\u003c\/p\u003e\n\u003cp\u003ePlease see manual for product P201\u003c\/p\u003e\n\u003ch2\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Achiever_TM_P201_Taq_Plus_DNA_Polymerase-GBS.pdf?v=1744128247\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"178\" height=\"50\"\u003e\u003c\/a\u003e\u003cbr\u003eDefinition of Activity Unit\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore all components at –20°C\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"250U","offer_id":39766538420324,"sku":"P201-01","price":43.89,"currency_code":"CAD","in_stock":true},{"title":"1000U","offer_id":39766538453092,"sku":"P201-02","price":153.63,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/AchieverDNAPolymerase.jpg?v=1669147313"},{"product_id":"100-mm-2-f-dntp-set","title":"100mM 2'-F-dNTP Set","description":"\u003cp\u003eA set of four 2'-F-dNTPs, with 100mM each of 2'-F-dATP, 2'-F-dCTP, 2'-F-dGTP, 2'-F-dUTP. RNAse, DNAse-free, high purity. \u0026gt;2 year Stability at -20 C. Can be used in RNA synthesis reactions to produce RNA with increased stability. \u003c\/p\u003e\n\u003cp\u003eIf you need other sizes, please  talk to us. \u003c\/p\u003e\n\u003cp\u003e\u0026gt;93% in purity. \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"2mL","offer_id":39766538616932,"sku":"PCN03-01","price":3950.45,"currency_code":"CAD","in_stock":true},{"title":"4mL","offer_id":39766538649700,"sku":"PCN03-02","price":5706.22,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/228.jpg?v=1643677173"},{"product_id":"long-pcr-taq-master-mix-with-optimizer","title":"Long PCR Taq Master Mix with Optimizer","description":"\u003cp\u003e\u003cspan\u003eThis product is a 2X Long PCR reagent Mix, plus a separately supplied Long PCR DNA Polymerase. The mix contains dNTPs, Mg2+ and Buffer at optimal concentrations. This Optimzer format provides flexibility of optimizing Long DNA polymerase activity for each unique reaction and still the convenience that comes with master mixes. The Long Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex targets, such as GC-rich targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eLong PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, \u003c\/span\u003ewhich can be used in TA clone\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e 5 x 1 ml is supplied. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR amplification of DNA fragments any sizes around 5 kb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR for cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High fidelity: three times fidelity of Taq DNA Polymerase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Longer fragment: amplify long templates as long as 40kb.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Amplification of complex template (GC rich or repetitive sequence).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Generates 3'-dA and blunt end PCR products. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10 x Long PCR Buffer is classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10 x Long PCR Buffer \u003cspan\u003eⅡ\u003c\/span\u003e is an alternative long PCR buffer . It is for better fidelity but may not be robust for longer templates above 10 kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNotes on cycling conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e- Initial denaturation can be performed over an interval of 1~5 min at 95\u003cspan\u003e℃\u003c\/span\u003e depending on the GC content of template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Denaturation for 30 sec to 2 min at 94~95\u003cspan\u003e℃\u003c\/span\u003e is sufficient. If the amplified DNA has a very high GC content, denaturation time may be increased up to 4 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Optimal annealing temperature is 5\u003cspan\u003e℃\u003c\/span\u003e lower than the melting temperature of primer-temperature DNA duplex. If nonspecific PCR products are obtained optimization of annealing temperature can be performed by increasing temperature stepwise by 2\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The number of PCR cycles depends on the amount of emplate DNA in the reaction mix and on the expected yield of the PCR products, 25-35 cycles are usually sufficient \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003efor the majority PCR reaction. Low amounts of starting template may require 40 cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The time of the final extensi, on step can be extended for amplicons that will be cloned into T\/A vectors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766538748004,"sku":"P306","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/229.jpg?v=1643677180"},{"product_id":"gb-amp-taq-dna-polymerase","title":"GB-Amp™ Taq DNA Polymerase","description":"\u003cp\u003e\u003cstrong\u003e\u003cem\u003eOther sizes of this product are also available. Please inquire. \u003c\/em\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eGB-Amp™ \u003c\/span\u003eTaq DNA Polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5' → 3' DNA polymerase and a 5' → 3' exonuclease activity but lacks a 3' → 5' exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase is heat-stable.\u003c\/p\u003e\n\u003cp\u003eContents\u003c\/p\u003e\n\u003cp\u003eTaq DNA Polymerase by default is packaged in 5 U\/μl.\u003c\/p\u003e\n\u003cp\u003e10×PCR Buffer with Mg++, \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eApplications\u003cbr\u003eStandard PCR \u003cbr\u003eDNA labeling \u003cbr\u003eDNA sequencing \u003cbr\u003eNumerous applications for which a high-quality, thermostable DNA polymerase is required\u003cbr\u003e\u003cbr\u003eUnit Definition\u003cbr\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.\u003cbr\u003e\u003cbr\u003eStorage Buffer\u003c\/p\u003e\n\u003cp\u003e20mM TrisCl ( pH8.0), 100mM KCl, 3.2mM MgCl2 1mM DTT，0.1% Triton X-100 ,0.1% Tween20, 0.2mg\/ml BSA, 50% (v\/v) glycerol\u003c\/p\u003e\n\u003cp dir=\"ltr\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eNote\u003c\/p\u003e\n\u003cp\u003e-Recombinant Taq DNA Polymerase is the enzyme of choice for most PCR applications.\u003c\/p\u003e\n\u003cp\u003e-The half-life of enzyme is \u0026gt;40 minutes at 95°C.\u003c\/p\u003e\n\u003cp\u003e-The error rate of Taq DNA Polymerase in PCR is 2.2x10-5 errors per nt per cycle;\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003ethe accuracy (an inverse of the error rate) an average number of correct nucleotides incorporated before making an error, is 4.5x104.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e- Taq DNA Polymerase accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labeled nucleotides) as substrates for the DNA synthesis.Compatible with TA cloning – generates PCR products with 3’-dA overhangs.\u003c\/p\u003e\n\u003cp\u003e-Suitable for Taqman probe real time PCR\u003c\/p\u003e\n\u003cp\u003e-Recommendations with Template DNA in a 50μl reaction volume\u003c\/p\u003e\n\u003cp\u003e-recommended quantities of template per react\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"blob.png\" title=\"59b9137604a11.png\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/t\/6\/assets\/59b9137604a11.png?v=1643677186\"\u003e\u003c\/p\u003e\n\u003cp\u003eStore all components at –20°C\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GBS-P101_Taq_DNA_Polymerase_Mg2_plus_buffer.pdf?v=1709224988\"\u003e\u003cspan\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cstrong\u003e\u003cimg data-mce-fragment=\"1\" height=\"52\" width=\"185\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1000U","offer_id":39766538879076,"sku":"P101-01","price":105.42,"currency_code":"CAD","in_stock":true},{"title":"5000U","offer_id":39766538911844,"sku":"P101-02","price":419.13,"currency_code":"CAD","in_stock":true},{"title":"10,000U","offer_id":39766538944612,"sku":"P101-03","price":709.97,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/GB-AmpTaqDNAPolymerase.jpg?v=1669147237"},{"product_id":"benzonase-nuclease-250-u-ul","title":"Benzonase Nuclease, 250U\/µl","description":"\u003cp\u003e\u003cspan\u003eBenzonase Nuclease, No-specific endonuclease, 250 U\/µl, is a genetically engineered endonuclease from \u003cem\u003eSerratia marcescens\u003c\/em\u003e. Benzonase® Nuclease. It is a highly useful endonuclease for removal of nucleic acids DNA and RNA, from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)\/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.\u003cbr\u003e\u003cbr\u003e Unit definition:\u003cbr\u003e One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units. \u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e• It possesses a higher specific activity than other nucleases\u003c\/p\u003e\n\u003cp\u003e• It is free of detectable proteolytic activities\u003c\/p\u003e\n\u003cp\u003e• It can be used effectively in various fields of applications, including applications in biotechnological processing, such as recombinant protein expression and purification, and biopharmaceutical production • It has a proven track record in various commercial vaccine manufacturing processes. \u003cstrong\u003eBulk quantities are available. GMP grade is also available. See our GMP section for more information. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GBS-Benzonase_User_Manual-Copy.pdf?v=1705431907\"\u003e\u003cstrong\u003e\u003cspan data-mce-fragment=\"1\"\u003e\u003cimg data-mce-fragment=\"1\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"153\" height=\"43\" data-mce-selected=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000U","offer_id":39766539305060,"sku":"OTD-01","price":54.87,"currency_code":"CAD","in_stock":true},{"title":"25,000U","offer_id":39766539337828,"sku":"OTD-02","price":252.39,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/noimagedefaultimage-jpeg_79486e0d-7800-4dc4-9560-d3b37d870074.jpg?v=1647396538"},{"product_id":"gb-amp-hotstart-taq-2x-master-mix","title":"GB-Amp™ HotStart Taq 2X Master Mix","description":"\u003cdiv\u003eUsed for end-point PCR, this product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3' end of the PCR product, A base, can be directly cloned into the T vectors (e.g., our TA-cloning kits: \u003ca href=\"https:\/\/www.genebiosystems.com\/50-ta-cloning-kits\"\u003ehttps:\/\/www.genebiosystems.com\/50-ta-cloning-kits\u003c\/a\u003e)\u003c\/div\u003e\n\u003cp\u003eWhen using this product for qPCR reactions, reliable standard curve can be obtained in a wide range and accurate qualification of target genes can be achieved with high repeatability and reliability.\u003c\/p\u003e\n\u003cp\u003ePlease note: this product does not contain loading dye for gel electrophoresis, therefore, loading buffer with dye must be added before loading PCR products onto gels. \u003c\/p\u003e\n\u003cp\u003eFor the HotStart Taq 2 x Master Mix pre-spiked with loading dye, please refer to GB-Amp™ HotStart Taq 2x Master Mix (with Loading Dye) \u003clabel\u003eCat. #:\u003c\/label\u003e \u003cspan class=\"editable\"\u003eP2041b \u003ca href=\"https:\/\/www.genebiosystems.com\/pcr-reagents\/260802-hot-start-taq-2x-master-mix.html\"\u003ehttps:\/\/www.genebiosystems.com\/pcr-reagents\/260802-hot-start-taq-2x-master-mix.html\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"editable\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003c\/h2\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39766539436132,"sku":"P2041","price":31.82,"currency_code":"CAD","in_stock":true},{"title":"5 ml","offer_id":39766539468900,"sku":"P2042","price":141.56,"currency_code":"CAD","in_stock":true},{"title":"10 ml","offer_id":39766539501668,"sku":"P2043","price":263.37,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_38f7efe8-962f-4d0a-b7fb-59ac67be3e78.jpg?v=1706709443"},{"product_id":"pcr-enhancing-reagent","title":"PCR Enhancing Reagent","description":"\u003cp\u003eThe PCR Enhancing Reagent is supplied in 10 x conc. Simply add the reagent in PCR reactions in the 1x-3x final concentration. We recommend that for each target, a series of reactions are set up to titrate the concentration of the PCR Enhancing Reagent in 1x, 2x, and 3 x concentrations.\u003c\/p\u003e\n\u003cp\u003eThe PCR Enhancing Reagent is also referred to as PCR Enhancer. \u003c\/p\u003e\n\u003cp\u003eThe effective melting temperature of primers in a reaction containing this reagent is reduced. Therefore, a decrease in annealing temperature of PCR is often required. \u003c\/p\u003e\n\u003cp\u003eWhat is this for? High GC content DNA targets or DNA targets with secondary structures. \u003c\/p\u003e\n\u003cp\u003eA low-cost way to troubleshoot difficult PCR amplification. Also try our high Robustness PCR enzymes, including the PaCeR. \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL","offer_id":41734492487780,"sku":"RNK3101","price":20.38,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":41734492520548,"sku":"RNK3102","price":83.09,"currency_code":"CAD","in_stock":true},{"title":"20mL","offer_id":41734492553316,"sku":"RNK3103","price":156.24,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/235.jpg?v=1643677207"},{"product_id":"2x-achiever-taq-master-mix-with-loading-dye","title":"2X Achiever™ Taq Master Mix (with loading dye)","description":"\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch2 data-end=\"175\" data-start=\"114\"\u003eProduct Details\u003c\/h2\u003e\n\u003cp class=\"\" data-end=\"563\" data-start=\"177\"\u003e\u003cstrong data-end=\"208\" data-start=\"177\"\u003e2X Achiever™ Taq Master Mix\u003c\/strong\u003e combines the high processivity of Taq polymerase with the superior fidelity of Pfu polymerase, delivering robust amplification performance even with complex templates. This dual-enzyme blend is ideal for amplifying longer fragments and GC-rich or structurally challenging DNA sequences—offering higher fidelity and greater yields than standard Taq alone.\u003c\/p\u003e\n\u003cp class=\"\" data-end=\"792\" data-start=\"565\"\u003eThe pre-mixed version includes a blue loading dye, allowing PCR products to be loaded directly onto an agarose gel for electrophoresis. The dye has been thoroughly validated to ensure it does not interfere with PCR performance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"952\" data-end=\"971\"\u003eHigher Fidelity\u003c\/strong\u003e – 4x greater fidelity than standard Taq polymerase\u003cbr data-start=\"1022\" data-end=\"1025\"\u003e\u003cstrong data-start=\"1027\" data-end=\"1042\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"1027\" data-end=\"1042\"\u003eHigh Yields\u003c\/strong\u003e – Efficient amplification of GC-rich, repetitive, or structurally complex templates\u003cbr data-start=\"1126\" data-end=\"1129\"\u003e\u003cstrong data-start=\"1131\" data-end=\"1154\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"1131\" data-end=\"1154\"\u003eLong PCR Capability\u003c\/strong\u003e – Amplifies up to 10 kb from genomic DNA and up to 20 kb from plasmid or phage DNA\u003cbr data-start=\"1237\" data-end=\"1240\"\u003e\u003cstrong data-start=\"1242\" data-end=\"1267\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"1242\" data-end=\"1267\"\u003eTA Cloning-Compatible\u003c\/strong\u003e – Produces PCR products with 3’-dA overhangs, ideal for TA cloning\u003cbr data-start=\"1334\" data-end=\"1337\"\u003e\u003cstrong data-start=\"1339\" data-end=\"1360\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"950\" data-end=\"1413\"\u003e\n\u003cstrong data-start=\"1339\" data-end=\"1360\"\u003eConvenient Format\u003c\/strong\u003e – Includes blue loading dye for direct gel loading\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1420\" data-end=\"1445\"\u003eQuality Assurance\u003c\/h2\u003e\n\u003cul data-start=\"1446\" data-end=\"1551\"\u003e\n\u003cli data-start=\"1446\" data-end=\"1488\" class=\"\"\u003e\n\u003cp data-start=\"1448\" data-end=\"1488\" class=\"\"\u003eFree from endo\/exonucleases and RNases\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1446\" data-end=\"1488\" class=\"\"\u003e\n\u003cp data-start=\"1448\" data-end=\"1488\" class=\"\"\u003eFunctionally tested in PCR for performance and reliability\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore all components at –20°C\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003ePlease see product protocols for \u003cspan\u003eP212-01 below:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Achiever_P211-P212_2x_Master_Mix-GBS.pdf?v=1700579180\"\u003e\u003cimg height=\"49\" width=\"174\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5mL","offer_id":39766541860964,"sku":"P212-01","price":164.61,"currency_code":"CAD","in_stock":true},{"title":"15mL","offer_id":39766541893732,"sku":"P212-02","price":384.07,"currency_code":"CAD","in_stock":true},{"title":"50mL","offer_id":39766541926500,"sku":"P212-03","price":986.52,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/image-removebg-achieverwithloadingdye.png?v=1680988362"},{"product_id":"proteinase-k-lyophilized","title":"Proteinase K  Lyophilized Powder","description":"\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003eProteinase K, lyophilized, Specific activity ≥30 U\/mg protein, is a broad substrate non-specific serine proteinase. It is very stable at pH 4-12 and can be used in isolating mRNA, genomic DNA and digesting unwanted proteins during DNA and RNA preparations from tissues and cells. It’s also been used on glycoprotein modification and protein structure studies. Proteinase K is active with SDS, urea and EDTA. the very agents that inactivates nucleases.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eProteinase K is used for the isolation of native high molecular genomic nucleic acids. Enzymes like DNases and RNases from microorganisms and mammalian cells are rapidly inactivated by Proteinase K.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecific Activity:\u003c\/strong\u003e \u003cstrong\u003e\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e≥3\u003c\/span\u003e0 U\/mg protein\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSource: \u003c\/strong\u003eTritirachium album limber\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e 29,730\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCAS: \u003c\/strong\u003e39450-01-6\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEINECS No:\u003c\/strong\u003e3.4.21.64\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePurity:\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e≥\u003c\/span\u003e\u003c\/strong\u003e95%\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder. \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eStorage Conditions:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e Store vial at 2-4ºC upon receipt.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDissolve and aliquot and store at -20ºC recommended.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eUse sterile filtration for liquid enzyme storage if keep it at 2-8ºC over a week.\u003c\/p\u003e\n\u003cp\u003eStorage Buffer: 20 mM Tris-HCl (pH 7.4), 1 mM CaCl2, 50% Glycerol (Dilution buffer without glycerol). QC Assays: Unit Definition: One unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37ºC, pH7.5.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/DNase%20I.html\" data-mce-href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/DNase%20I.html\"\u003eDNase\u003c\/a\u003e Activity: None detectable enzyme activity with λ DNA after 6 hrs incubation at 37ºC.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/RNase%20A.html\" data-mce-href=\"http:\/\/www.mebep.com\/a\/Products\/Enzyme\/2013\/0823\/RNase%20A.html\"\u003eRNase\u003c\/a\u003e Activity: None detectable ribonuclease activity after 16 hrs incubation at 25ºC.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eAdding Proteinase K already during the cell lysis enables the isolation of highly native undamaged high molecular DNA or RNA. A variety of methods have been established, which are documented in numerous publications.\u003cbr\u003e\u003cbr\u003eRecently, Proteinase K has been used for the detection of BSE forming proteins which are uniquely resistant towards the enzyme's proteolytic cleavage.\u003cbr\u003e\u003cbr\u003eProteinase K is very useful in the analysis of membrane structure by means of modification of proteins and glycoproteins on cell surfaces.\u003cbr\u003e\u003cbr\u003eBecause of the cleavage specificity Proteinase K, characteristic fragments of proteins are obtained which are helpful in revealing the structure and function of proteins, particularly enzymes.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Proteinase_K-N9016-Lyophilized_User_Manual.pdf?v=1709225452\" data-mce-href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Proteinase_K-N9016-Lyophilized_User_Manual.pdf?v=1709225452\"\u003e\u003cspan\u003e\u003cspan style=\"text-decoration: underline;\" data-mce-style=\"text-decoration: underline;\"\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"185\" height=\"52\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-fragment=\"1\"\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 mg","offer_id":39766542712932,"sku":"N9016","price":44.55,"currency_code":"CAD","in_stock":true},{"title":"1 g","offer_id":39766542745700,"sku":"N9017","price":229.68,"currency_code":"CAD","in_stock":true},{"title":"5 g","offer_id":39766542778468,"sku":"N9018","price":613.75,"currency_code":"CAD","in_stock":true},{"title":"20 g","offer_id":41950700339300,"sku":"N9019","price":2621.4,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/269.jpg?v=1643677242"},{"product_id":"low-retention-pipette-tips-in-bulk-bagged","title":"Non-filtered Low-retention Pipette Tips in Bulk-Bagged","description":"\u003ch2\u003eFeatures include:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli style=\"list-style-type: none;\"\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003e1000 tips per bag\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eAutoclavable\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eEngineered for increased accuracy and are compatible with most single-channel, multichannel, and electronic pipetters\u003c\/li\u003e\n\u003cli\u003eDNase-\/RNase-free;\u003c\/li\u003e\n\u003cli\u003eTips allow for noncontaminated sample recovery\u003c\/li\u003e\n\u003cli\u003eAvailable in 10, 200, 300, 1000 and 1250 ul volume ranges (prices and sizes are provided as options on the right side of this page)\u003c\/li\u003e\n\u003cli\u003eMade of high transparency PP material, the tip is straight with high accuracy\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eNote: These tips do not have filters. For filter pipettes, choose our filter tips for either loose (bulk) filter tips, or racked filter tips in 96-count boxes. \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"200μL Tips","offer_id":39766543695972,"sku":"MTB200R-10L","price":22.36,"currency_code":"CAD","in_stock":false},{"title":"1000μL Tips","offer_id":39766543728740,"sku":"MTB1000R-10L","price":28.45,"currency_code":"CAD","in_stock":true},{"title":"1250μL Tips","offer_id":39863000039524,"sku":"MTB1250R-10L","price":31.5,"currency_code":"CAD","in_stock":true},{"title":"10μL XL Tips","offer_id":39766543663204,"sku":"MTB10R-10L","price":22.36,"currency_code":"CAD","in_stock":false},{"title":"300μL Tips","offer_id":39862998335588,"sku":"MTB300R-10L","price":28.45,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/528.jpg?v=1643677263"},{"product_id":"fs-fast-dna-polymerase-2x-master-mix","title":"FS Fast DNA Polymerase 2X Master Mix","description":"\u003cp\u003e\u003cspan\u003eThe same high-performance Taq DNA polymerase, configured in a Master Mix format for your convenience. Fast Taq DNA polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be used in a variety of PCR. The FS\u003csup\u003eTM\u003c\/sup\u003e PCR Buffer, designed for FS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA polymerase, can be used in fast amplification reaction. The elongation rate of FS\u003csup\u003eTM\u003c\/sup\u003e Taq DNA polymerase is more than twice the rate by regular Taq DNA polymerase, which shortens the amplification time at least by half , as fast as \u003cspan\u003e5s\/kb for simple sequence templates\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e Features\u003cbr\u003e Fast rate of elongation: elongation rate can reach to 3 kb\/min, more than twice the rate by regular Taq DNA polymerase;\u003cbr\u003e Highly thermostable : have a half-life of over 40 min at 95\u003cspan\u003e°\u003c\/span\u003eC incubation Generates 3'-dA overhangs PCR products.\u003cbr\u003e\u003cbr\u003e Applications\u003cbr\u003e Routine PCR\u003cbr\u003e PCR labeling\u003cbr\u003e PCR sequencing\u003cbr\u003e Generate PCR product for TA cloning\u003cbr\u003e\u003cbr\u003e Unit Definition\u003cbr\u003e One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70\u003cspan\u003e°\u003c\/span\u003eC using hering sperm DNA as substrate.\u003cbr\u003e\u003cbr\u003e Quality Control \u003cbr\u003e The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFunctionally tested in amplification of a single-copy gene from human genomic DNA\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore all components at \u003cspan\u003e–\u003c\/span\u003e20\u003cspan\u003e°\u003c\/span\u003eC\u003cbr\u003e\u003cbr\u003e Protocol for PCR\u003cbr\u003e All solutions should be thawed on ice, gently vortexed and briefly centrifuged.\u003cbr\u003e Add in a thin walled PCR tube on ice:\u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39766543892580,"sku":"P2070","price":43.89,"currency_code":"CAD","in_stock":true},{"title":"5 ml","offer_id":39766543925348,"sku":"P2070","price":164.61,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/242.jpg?v=1643677274"},{"product_id":"rt-pcr-kit-cdna-synthesis-kit","title":"RT-PCR Kit ( cDNA synthesis kit)","description":"\u003cdiv class=\"body\"\u003e\n\u003cdiv class=\"w-container w-main\"\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"wrap-content-in w-system w-productcom\"\u003e\n\u003cdiv class=\"w-system-in clearfix\"\u003e\n\u003cdiv class=\"product-detail-wrap\"\u003e\n\u003cdiv class=\"w-com-content\"\u003e\n\u003cdiv class=\"product-maincon\"\u003e\n\u003cdiv class=\"product-maincon-in\"\u003e\n\u003cdiv class=\"product-descons\"\u003e\n\u003cdiv class=\"descon_item\" id=\"Tabitem234864\"\u003e\n\u003cp\u003e\u003cspan\u003eRT-PCR Kit is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The RT-pcr kit for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. You get all of the components you need for successful first-strand cDNA synthesis, saving your time and ensuring your success with every experiment. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42-55\u003cspan\u003e°\u003c\/span\u003eC, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications \u003cbr\u003eFirst strand cDNA synthesis for RT-PCR . \u003cbr\u003eConstruction of cDNA libraries. \u003cbr\u003eOne-step RT-PCR \u003cbr\u003ePrimer extension\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eComponents of the kit \u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eCompenent\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eµl\u003cstrong\u003e (20 rxns)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eµl\u003cstrong\u003e (100 rxns)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eM-MLV (200 U\/µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRNAsine (40 U\/ µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e12\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e60\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eOligo (dT)15 (50 µM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRandom hexame (50 µM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e100\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5x First-strand buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e80\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e400\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRnase-free ddH2O\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml x 5\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003edNTP (10 mM each)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e200\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStorage\u003cbr\u003eAll components of the kit should be stored at -20\u003cspan\u003e°\u003c\/span\u003eC. Keep control RNA at -70\u003cspan\u003e°\u003c\/span\u003eC for longer storage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eImportant notes \u003cbr\u003eAvoiding ribonuclease contamination \u003cbr\u003eRNA purity and integrity is essential for synthesis of full-length cDNA. RNA can be degraded RNase A, which is a highly stable contaminant found in any laboratory environment. All components of the kit have been rigorously tested to ensure that they are RNase free. To prevent contamination both the laboratory environment and all prepared solutions must be free of RNases. General recommendations to avoid RNase contamination:\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eDEPC-treat all tubes and pipette tips to be used in cDNA synthesis or use certified nuclease-free labware.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eWear gloves when handling RNA and all reagents, as skin is a common source of RNases. Change gloves frequently.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eUse RNase-free reagents, including high quality water (e.g., DEPC-treated Water).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eUse an RNase inhibitor to protect RNA from the activity of RNases.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eKeep all kit components tightly sealed when not in use. Keep all tubes tightly closed during the reverse transcription reaction.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cspan\u003eTemplate RNA \u003cbr\u003eTotal cellular RNA isolated by standard methods is suitable for use with the kit. Purified RNA must be free of salts, metal ions, ethanol and phenol to avoid inhibiting the cDNA synthesis reaction. Trace contaminants can be removed by ethanol precipitation of the RNA followed by two washes of the pellet with cold 75% ethanol. For RT-PCR applications, template RNA must be free of DNA contamination. Prior to cDNA synthesis, RNA can be treated with DNase I to remove trace amounts of DNA (DNase I is not included in this kit). Always perform a control (RT-minus) reaction which includes all components for RT-PCR except for the reverse transcriptase enzyme.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRNase H Digestion \u003cbr\u003eThe sensitivity of the PCR step can be increased (especially for long templates) by removing the RNA template from the cDNA:RNA hybrid molecule by digestion with RNase H after first-strand synthesis. Presence of RNase H during first-strand synthesis will degrade the template mRNA, resulting in decreased full-length cDNA synthesis and decreased yields of first-strand cDNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePrimers \u003cbr\u003eSynthesis of first strand cDNA can be primed with either oligo(dT)15 primer, random primers or gene-specific primers.\u003cbr\u003eOligo(dT)15 primes cDNA synthesis from the poly(A) tail present at the 3\u003cspan\u003e’\u003c\/span\u003e-end of eukaryotic mRNA. Random Primers initiate cDNA synthesis from the total RNA population (rRNA and mRNA). Therefore, using random primers for first strand synthesis results in a greater complexity of the generated cDNA compared with the oligo(dT)15 primer. As a consequence, the sensitivity and specificity of subsequent PCR reactions may be reduced. However, there are several applications where it is beneficial to use random primers, such as cDNA synthesis using mRNAs without a poly(A) tail, or cDNA synthesis using poly(A)-enriched RNA samples.\u003cbr\u003eGene-specific primers are used to synthesize specifi c cDNA from a pool of total RNA or mRNA and must be obtained by the user.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFirst Strand cDNA synthesis procedure \u003cbr\u003eThe first strand cDNA reaction can be performed as an individual reaction or as a series of parallel reactions with different RNA templates. Therefore, the reaction mixture can be prepared by combining reagents individually or a master mix containing all of the components except template RNA can be prepared. Depending on the structure of the RNA template, separate steps for RNA denaturation and primer annealing may improve RT-PCR results.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProtocol \u003cbr\u003eI. First strand cDNA synthesis\u003cbr\u003e1. Add the following reagents into a sterile, nuclease-free tube on ice in the indicated order:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eTemplate RNA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePoly (A) mRNA or specific RNA\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1-5 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eOligo (dT)15 Primer or Randome hexamer primer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µg\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eDEPC-treated water\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd colspan=\"2\"\u003e\n\u003cp\u003e\u003cspan\u003e To 12.4 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e2. Mix gently, centrifugebriefly and incubate at 70\u003cspan\u003e°\u003c\/span\u003eC for 5 min. Chill on ice, spin down and place the vial back on ice.\u003cbr\u003e3. Prepare the following cDNA Synthesis Mix, add the following components in the indicated order:\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5x First-strand buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e4 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003edNTPs(10 mM each)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRnasine\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.6 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eM-MLV\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Mix gently and centrifuge\u003cbr\u003e5.For oligo(dT)15, incubate for 60 min at 42\u003cspan\u003e°\u003c\/span\u003eC. For random hexamer primed synthesis, incubate for 60 min at 37\u003cspan\u003e°\u003c\/span\u003eC .\u003cbr\u003e6.Terminate the reaction by heating at 70\u003cspan\u003e°\u003c\/span\u003eC for 5 min. \u003cbr\u003eThe reverse transcription reaction product can be used immediately in second strand cDNA synthesis reactions or stored at -20\u003cspan\u003e°\u003c\/span\u003eC for less than a week. For longer storage, -70\u003cspan\u003e°\u003c\/span\u003eC is recommended.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eII. PCR Amplification of First Strand cDNA\u003cbr\u003eThe product of the first strand cDNA synthesis can be used directly in PCR or qPCR. The volume of first strand cDNA synthesis reaction mixture should not comprise more than 1\/10 of the total PCR reaction volume. Normally, 2 \u003cspan\u003eμ\u003c\/span\u003el of the first strand cDNA synthesis reaction mixture is used as template for subsequent PCR in 50 \u003cspan\u003eμ\u003c\/span\u003el total volume.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R1011-R1012_RT-PCR_Kit_V1.0_1_-GBS.pdf?v=1700836884\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"160\" height=\"45\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"20 Preps","offer_id":39766544220260,"sku":"R1011","price":103.47,"currency_code":"CAD","in_stock":true},{"title":"100 Preps","offer_id":39766544253028,"sku":"R1012","price":354.29,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_ee950fd4-ff2b-49fa-b323-cba524f27fc4.jpg?v=1704394367"},{"product_id":"long-pcr-taq-polymerase-master-mix","title":"Long PCR Taq polymerase Master Mix","description":"\u003cp\u003e\u003cspan\u003eMaster mix Long PCR Taq DNA Polymerase, the same high-performing DNA polymerases, now in the master mix format to provide you with convenience of use. The master mix is a special formulation containing the Long PCR Taq DNA polymerase. The Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex targets, such as GC-rich targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eJust add primers and template, your PCR reaction is ready to begin!\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, \u003c\/span\u003ewhich can be used in TA clone\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR amplification of DNA fragments any sizes around 5 kb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR for cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High fidelity: three times fidelity of Taq DNA Polymerase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Longer fragment: amplify long templates as long as 40kb.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Amplification of complex template (GC rich or repetitive sequence).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Generates 3'-dA and blunt end PCR products. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer\u003cspan\u003eⅠ\u003c\/span\u003eis classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer \u003cspan\u003eⅡ\u003c\/span\u003e is an alternatie long PCR buffer . It is for better fidelity but may not be robust for longer templates above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eBasic PCR Protocol\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following basic protocol serves as a general guideline and a starting point for any PCR amplification. Optimal reaction conditions \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(incubation time and temperature, concentration of Taq DNA Polymerase, primers, Mg2+, and template DNA) vary and need to be optimized. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNotes on cycling conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e- Initial denaturation can be performed over an interval of 1~5 min at 95\u003cspan\u003e℃\u003c\/span\u003e depending on the GC content of template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Denaturation for 30 sec to 2 min at 94~95\u003cspan\u003e℃\u003c\/span\u003e is sufficient. If the amplified DNA has a very high GC content, denaturation time may be increased up to 4 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Optimal annealing temperature is 5\u003cspan\u003e℃\u003c\/span\u003e lower than the melting temperature of primer-temperature DNA duplex. If nonspecific PCR products are obtained optimization of annealing temperature can be performed by increasing temperature stepwise by 2\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The number of PCR cycles depends on the amount of emplate DNA in the reaction mix and on the expected yield of the PCR products, 25-35 cycles are usually sufficient \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003efor the majority PCR reaction. Low amounts of starting template may require 40 cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The time of the final extensi, on step can be extended for amplicons that will be cloned into T\/A vectors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL","offer_id":39766544351332,"sku":"DNK300P-01","price":76.81,"currency_code":"CAD","in_stock":true},{"title":"3mL","offer_id":39766544416868,"sku":"DNK300P-02","price":197.52,"currency_code":"CAD","in_stock":true},{"title":"6mL","offer_id":39766544449636,"sku":"DNK300P-03","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/245.jpg?v=1643677287"},{"product_id":"6x-gel-loading-buffer-with-dyes","title":"6x Gel Loading Buffer with Dyes","description":"\u003cp\u003e\u003cstrong\u003eQuantity:\u003c\/strong\u003e 5 x 1 mL tubes (5 mL total)\u003c\/p\u003e\n\u003cp\u003eGel Loading Buffer with Dye (6X) is a pre-mixed Loading Buffer with two tracking dyes for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping any ongoing reaction. Bromophenol blue and xylene cyanol are the standard tracking dye for electrophoresis.\u003cbr\u003e\u003cbr\u003eContents\u003cbr\u003eGel Loading Dye (6X):\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e60 mM Tris-HCL\u003cspan lang=\"zh-cn\"\u003e（\u003c\/span\u003epH 8.0\u003cspan lang=\"zh-cn\"\u003e）\u003c\/span\u003e\u003cbr\u003e0.06% Bromophenol Blue\u003cbr\u003e0.03% Xylene Cyanol\u003cbr\u003e60% Glycerol\u003cbr\u003e60 mM EDTA\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStorage\u003c\/p\u003e\n\u003cp\u003eStore at RT\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eQuality Assurance Statement\u003cbr\u003eGel Loading Dye(6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.\u003cbr\u003e\u003cbr\u003eDye Migrate Rate (on a standard 1% TAE or TBE agarose gel) \u003cbr\u003eXylene Cyanol\u003cbr\u003eTAE: 4160 bp\u003cbr\u003eTBE: 3030 bp\u003cbr\u003e\u003cbr\u003eBromophenol Blue \u003cbr\u003eTAE: 370 bp\u003cbr\u003eTBE: 220 bp\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/6x-Gel-Loading_Buffer-with-Dyes_M904_GBS-Manual_Jan-2025.pdf?v=1736359233\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/R323-V22.1-GBS.pdf?v=1700840382\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766544810084,"sku":"M904","price":23.04,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/246.jpg?v=1643677293"},{"product_id":"fast-taq-blood-direct-mix","title":"Fast Taq Blood Direct Mix","description":"\u003cp\u003e\u003cspan\u003eFS Taq Mix Direct for Blood is a premixed, ready-to-use solution containing FS Taq DNA Polymerase, dNTPs, and all other PCR components, except DNA template and primers. FS Taq Mix Direct for Blood is specific for whole blood amplification. It contributes to fast, specific, sensitive and reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. The FS Mix (2X) B can be used with conventional PCR machines. This direct protocol makes the process of complicated and time-consuming DNA extraction unnecessary, and also minimize the risk of cross-contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFS Taq DNA Polymerase is the latest generation Taq-based DNA polymerase. It possesses high amplification efficiency as does Taq polymerase, and fast elongation ability as does KOD polymerase, can be use in various kinds of PCR. The FS PCR Buffer is designed for FS Taq DNA polymerase, can be used in fast amplification reaction. FS Taq DNA polymerase has an elongation rate 2x higher than regular Taq DNA polymerase, and can shorten the amplification time by half. It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity, which results in a 3'-dA overhangs PCR product.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eContents\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2X Taq Mix Direct Mix B; Nuclease-free water\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote: The product contains bromophenol blue for convenient gel loading of the PCR products. If you would like to leave it out, talk to us when you order the product.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Convenient: Direct for whole blood amplification PCR\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e High yields of PCR products with minimal optimization\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Fast: saves time due to reduced number of pipetting steps \u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Reproducible: lower contamination and pipetting error risk \u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Higher sensitivity and fast compared to conventional Taq DNA polymerase\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e Amplification for Whole Blood\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e High throughput PCR\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Long and Complex template PCR\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003eUnit Definition\u003cbr\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70\u003cspan\u003e°\u003c\/span\u003eC using hering sperm DNA as substrate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eBasic PCR Protocol\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAll solutions should be thawed on ice, gently vortexed and briefly centrifuged.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1. Add in a thin walled PCR tube on ice:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003eFor a total 50\u003cspan\u003eμ\u003c\/span\u003el reaction volume\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eReagent\/Sample\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVolume (50 µl rxn)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFinal concentration\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10x FS PCR Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e25 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1x\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer I\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.1-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer II\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.1-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eBlood\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.2-2 µl*\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWater\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable to 50 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eN.A.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e*up to 5 \u003c\/span\u003e\u003cspan\u003eµl can be used. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eRecommendation for Blood Template in a 50\u003cspan\u003eμ\u003c\/span\u003el reaction volume is 0.1-1\u003cspan\u003eμ\u003c\/span\u003el.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Gently vortex the sample and briefly centrifuge to collect all drops to the bottom of the tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Overlay the sample with mineral oil or add an appropriate amount of wax. This step may be omitted if the thermal cycler is equipped with a heated lid.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. Preform PCR using the following thermal cycling conditions.\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eInitial Denaturation\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e94°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e4 min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e25-35 Cycles\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e94°C \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e55-68°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e72°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e30s\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e30s\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e20 s\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFinal Extension\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e72°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e2 min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1 ml","offer_id":39766544908388,"sku":"P207A","price":109.74,"currency_code":"CAD","in_stock":true},{"title":"5 ml","offer_id":39766544941156,"sku":"P207A","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/247.jpg?v=1643677297"},{"product_id":"rnase-a-powder","title":"RNase A-powder","description":"\u003cp\u003ePancreatic Ribonuclease (RNase  A ) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Activity \u003cspan class=\"font6\"\u003e≥\u003c\/span\u003e\u003cspan class=\"font5\"\u003e 50 KU\/mg peotein, Lyophilized Powder\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eSource: \u003c\/em\u003e\u003cem\u003eBovine Pancrease EC:3.1.27.5\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eThe ultrapure Pancreatic Ribonuclease has been further purified by affinity chromatography method to remove trace contaminating proteases.\u003c\/p\u003e\n\u003cp\u003eForm: lyophilized powder\u003c\/p\u003e\n\u003cp\u003eSPECIFICATION (I)\u003c\/p\u003e\n\u003cp\u003eActivity ≥ 50 Kunitz units\/mg powder\u003c\/p\u003e\n\u003cp\u003eSTORAGE Store in an airtight container, at a temperature of 2°C to 8°C.\u003c\/p\u003e\n\u003ch3\u003e\u003c\/h3\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 mg","offer_id":39766545039460,"sku":"N9046","price":61.67,"currency_code":"CAD","in_stock":true},{"title":"1 g","offer_id":39766545072228,"sku":"N9047","price":479.71,"currency_code":"CAD","in_stock":true},{"title":"5 g","offer_id":39766545104996,"sku":"N9047b","price":2063.02,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/249.jpg?v=1643677301"},{"product_id":"plexprep-3-rna-dna-mirna-extract-kit","title":"PlexPrep-3 RNA-DNA-miRNA Extract Kit","description":"\u003cp\u003eThe Kit is used to extract and collect RNA,DNA and miRNA separately from blood. Kits of 50 or 100 preps are available.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766545203300,"sku":"RNK4801","price":438.94,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766545236068,"sku":"RNK4801","price":768.14,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/250.jpg?v=1643677306"},{"product_id":"serological-pipettes","title":"Serological Pipettes","description":"\u003cdiv\u003e\n\u003cblockquote\u003e\n\u003ch3\u003e\u003cspan style=\"text-decoration: underline;\"\u003eAvailable in six volume capacities:\u003c\/span\u003e\u003c\/h3\u003e\n\u003ch4\u003e1.0mL, 2.0mL, 5.0mL, 10.0mL, 25.0mL and 50mL\u003c\/h4\u003e\n\u003c\/blockquote\u003e\n\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eMade of \u003cstrong\u003epremium quality polystyrene (GPPS)\u003c\/strong\u003e in class 100,000 clean room workshops\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eNovel aspiration area design can fit most of the pipettors on the market\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eUsed for accurate measure and\/or transfer of aqueous liquid and solutions \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eWidely used in the field of cell culture, bacteriology, clinical research, etc.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eGraduations are \u003cstrong\u003ecalibrated to within \u003cem data-mce-fragment=\"1\"\u003e± \u003c\/em\u003e2.0%\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003eSmooth and clean without drawing lines\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003eDifferent colors (color-coded)\u003c\/span\u003e\u003c\/strong\u003e, easy to distinguish minimizing errors\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eMaximally reduces the liquid adhesion to the pipette inner wall, improved transfer accuracy\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eExcellent \u003cstrong\u003edouble-side graduation design\u003c\/strong\u003e ideally suited for transferring entire volume or a volume by subtraction\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eClear and simple graduation, positive and negative ones\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eAll serological pipettes are assembled with high quality filter plugs\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eStringent integrity test on every pipette\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003eSterilized\u003c\/strong\u003e by gamma irradiation\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eNon-pyrogenic \u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eDNase\/RNase-free\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL, 500 Pieces","offer_id":39766545367140,"sku":"MSP0101","price":96.52,"currency_code":"CAD","in_stock":true},{"title":"2mL, 500 Pieces","offer_id":39766545399908,"sku":"MSP0201","price":101.61,"currency_code":"CAD","in_stock":true},{"title":"5mL, 400 Pieces","offer_id":39766545432676,"sku":"MSP0501","price":97.54,"currency_code":"CAD","in_stock":false},{"title":"10mL, 400 Pieces","offer_id":39766545465444,"sku":"MSP1001","price":100.59,"currency_code":"CAD","in_stock":false},{"title":"25mL, 200 Pieces","offer_id":39766545498212,"sku":"MSP2501","price":89.41,"currency_code":"CAD","in_stock":true},{"title":"50mL, 100 Pieces","offer_id":39766545530980,"sku":"MSP5001","price":96.52,"currency_code":"CAD","in_stock":true},{"title":"100mL 100 pieces","offer_id":41846605643876,"sku":"MSP100001","price":220.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_8e738572-c8f0-4d59-a0ef-013c2fc136a4.jpg?v=1708626612"},{"product_id":"mieasy-kit-for-paxgene-tube","title":"miEASY Kit (for PAXgene tube)","description":"\u003cdiv id=\"divDescription\"\u003e\n\u003cdiv id=\"productHead\" class=\"productHead\"\u003e\n\u003cp\u003eFor purification of miRNA and total RNA from whole blood\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"divFeatures\"\u003e\n\u003cdiv id=\"productFeatures\" class=\"dottedList\"\u003e\n\u003cul\u003e\n\u003cli\u003eEffective purification of miRNA and total RNA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"divShortinfo\"\u003e\n\u003cdiv id=\"productShortInfo\" class=\"copy\"\u003e\n\u003cp\u003eThe PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out using a microcentrifuge. Used with the tubes, the kit provides a complete pre-analytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766545662052,"sku":"RNK0501","price":263.37,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766545694820,"sku":"RNK0501","price":482.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/251.jpg?v=1643677320"},{"product_id":"m-mlv-reverse-transcriptase","title":"M-MLV Reverse Transcriptase","description":"\u003cp\u003e\u003cstrong style=\"font-size: 0.875rem;\"\u003eThe MMLV \u003c\/strong\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eReverse Transcriptase(short name MMLV) i is iolated from E.coli carrying rat leukemia virus pol gene consists of a single peptide, molecule weight 71kd.It possesses RNA and DNA depended polymerase activity and weak RNase H activity.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eConcentration: \u003c\/strong\u003e 200U\/μl\u003cbr\u003e\u003cstrong\u003eComponent : \u003c\/strong\u003e M-MLV （200U\/μl） 5×Buffer （with DTT）\u003cbr\u003e\u003cstrong\u003ePackage：\u003c\/strong\u003e Bulk\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003cbr\u003eWeak RNaseH activity; High cDNA yield\u003cbr\u003e\u003cstrong\u003eStoreage: \u003c\/strong\u003e-20℃\u003cbr\u003eApplication：Synthesis of the first chain cDNA, cDNA Library construction, one-step RT-PCR, primer extention, 3′ and 5′RACE\u003cbr\u003e\u003cstrong\u003eSource:\u003c\/strong\u003e Recombination of E.coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine .\u003cbr\u003e\u003cstrong\u003eUnit definition:\u003c\/strong\u003eOne unit is defined as the required enzyme incorporate 1 nm dNTP into a polynucleotide fraction in 10 min at 37℃, taking polyA﹒poly(dT)12-18 as template-primer.\u003cbr\u003e\u003cstrong\u003eProcedure:\u003c\/strong\u003e\u003cbr\u003e1. add the next reaction mixture to ice bath tube :\u003cbr\u003e1) template RNA\u003cbr\u003etotal RNA 0.1-5μg\u003cbr\u003eor total poly(A)+mRNA 0.1-0.5μg\u003cbr\u003eor unique RNA 0.01pg-0.5μg\u003cbr\u003e2) primer\u003cbr\u003eOligo(dT)18 (0.5μg\/μl) 1μl\u003cbr\u003eOr stochastic primer（0.2μg\/μl） 1μl\u003cbr\u003eOr sequence especially primer 20pmol\u003cbr\u003e3) RNase-free ddh2o : constant volume to 11μl\u003cbr\u003e2. Gently mix and water bath for 5 min in 70℃ and chill on ice.\u003cbr\u003e3. Put the tube into ice and add the next composition :\u003cbr\u003e5×Reaction Buffer 4μl\u003cbr\u003eRNase Inhibitor (40U\/μl) 0.5μl\u003cbr\u003edNTP Mix(10mmol\/L) 2μl\u003cbr\u003eadd water to 19ul ,gently mix and then water bath for 5 min in 37℃ ; or for 5 min in 25℃ for random primer \u003cbr\u003e4. Spin down for a few seconds. Add 1μl M-MLV RT（200U\/μl）\u003cbr\u003e5. Incubate at 42℃ for 60min(if use a random primer,first incubate for 10min in 25℃\u003cbr\u003e6. Inactivate at 70℃ for 10min.\u003cbr\u003e\u003cstrong\u003ePCR Reaction\u003c\/strong\u003e\u003cbr\u003e1. Transfer 10% volume of first reaction solution (2 μl ) to a proper PCR tube .\u003cbr\u003enote: the first reaction solution can be directly used as PCR template without purification ,the dosage is about 1-5μl. if excessively used ,the salt and Random primers in first reaction solution will restrain the activity of DNA polymerase .if purification needed ,it can follow the next :after reaction end of cDNA synthesis (step 6) , add RNase A in reaction system , 10 min in 37℃ ,use DP1501 recover cDNA .\u003cbr\u003e2. add next solution by order .\u003cbr\u003e5μl 10X PCR Buffer\u003cbr\u003e1μl 10mM dNTP mix\u003cbr\u003e1μl 10μM Primer #1 (customer supplied)\u003cbr\u003e1μl 10μM Primer #2 (p customer supplied)\u003cbr\u003exμl H20 (total reaction volume:49μl)\u003cbr\u003e1μl Taq DNA polymerase\u003cbr\u003e3. Mix thoroughly and add 50μl mineral oil to the surface of liquid.\u003cbr\u003e4. Amplified reaction : according to annealing temperature or gene copy number or technical parameter of Taq DNA polymerase , setting amplified condition , specify reference to specification of DNA polymerase ,the usually cycle number is 30-35\u003cbr\u003e5 Detect the product in agarose containing EB or another fluorescent dye.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000U","offer_id":39766547136612,"sku":"R1041","price":87.8,"currency_code":"CAD","in_stock":true},{"title":"25000U","offer_id":39903278661732,"sku":"R1042","price":384.07,"currency_code":"CAD","in_stock":true},{"title":"50000U","offer_id":42761391505508,"sku":"R1043","price":702.31,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/SealingFilmGuide.png?v=1765475529"},{"product_id":"e-coli-uracil-dna-glycosylase-udg","title":"E. coli Uracil-DNA Glycosylase (UDG)","description":"\u003cp\u003e\u003cem\u003eE. coli\u003c\/em\u003e Uracil-DNA Glycosylase (UDG) is a recombinant enzyme purified from an \u003cem\u003eE. coli\u003c\/em\u003e strain that carries the UDG gene from \u003cem\u003eE. coli\u003c\/em\u003e.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"5000 U","offer_id":39766547431524,"sku":"OTP-01","price":197.52,"currency_code":"CAD","in_stock":true},{"title":"25,000 U","offer_id":39766547464292,"sku":"OTP-01","price":877.88,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_309d961b-e467-4d9b-90a9-fc0f0f735868.jpg?v=1706882257"},{"product_id":"15ml-conical-centrifuge-tubes","title":"15mL Conical Centrifuge Tubes (Polypropylene)","description":"\u003cul\u003e\n\u003cli\u003eDNAse free and RNAse free\u003c\/li\u003e\n\u003cli\u003eMade of high transparency PP material, adapted to microcentrifuges of all models\u003c\/li\u003e\n\u003cli\u003eWidely used in molecular biological, clinical chemical and biochemical research\u003c\/li\u003e\n\u003cli\u003eResistant to chemical corrosion and freezing damage\u003c\/li\u003e\n\u003cli\u003eFree of plasticizer, fungistatic, and heavy metal\u003c\/li\u003e\n\u003cli\u003eStable under high centrifugal force (12000g).\u003c\/li\u003e\n\u003cli\u003eAdapted to wide range of temperature from -80 to 121 C, no distortion is observed\u003c\/li\u003e\n\u003cli\u003eClear graduation on the wise wall for easy observation of liquid volume\u003c\/li\u003e\n\u003cli\u003eArea on the cap and white area on the tube wall for convenient marking and labelling\u003c\/li\u003e\n\u003cli\u003eAvailable in sterilized by E.O.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e**Rack packages are also available\u003clabel\u003e \u003c\/label\u003e\u003ca href=\"https:\/\/www.genebiosystems.com\/products\/15ml-conical-centrifuge-tubes-rack-package?variant=39771533639780\"\u003e#: CTB15-Rack-100 tubes\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"100 Tubes","offer_id":39766547824740,"sku":"CTB15-25-100","price":33.33,"currency_code":"CAD","in_stock":false},{"title":"500 Tubes","offer_id":39766547857508,"sku":"CTB15-25-500","price":126.69,"currency_code":"CAD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/15ml_conical_tubes.jpg?v=1742212823"},{"product_id":"gb-clean-plasmid-miniprep-kit","title":"GB-Clean™ Plasmid Miniprep Kit (HiPure Mini system)","description":"\u003ch2\u003e\n\u003cspan style=\"font-size: 0.875rem;\"\u003eDetails\u003c\/span\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe GB-Clean™ Plasmid Miniprep Kit (HiPure Mini system) provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. GB-Clean™ Plasmid Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of tris buffer or water.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFeatures\u003c\/td\u003e\n\u003ctd\u003eSpecifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMain Functions\u003c\/td\u003e\n\u003ctd\u003eIsolation up to 35μg plasmid DNA from 1-5ml bacterial culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eEnzyme digestion, sequencing, PCR, cloning, etc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification method\u003c\/td\u003e\n\u003ctd\u003eMini spin column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification technology\u003c\/td\u003e\n\u003ctd\u003eSilica technology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProcess method\u003c\/td\u003e\n\u003ctd\u003eManual (centrifugation or vacuum)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample type\u003c\/td\u003e\n\u003ctd\u003eConventional plasmid, plasmid less than 30KB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample amount\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003eHigh copy plasmid: 1-5ml culture medium\u003c\/p\u003e\n\u003cp\u003eLow copy number plasmid : 5-10ml culture medium\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYield\u003c\/td\u003e\n\u003ctd\u003e5-35µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume\u003c\/td\u003e\n\u003ctd\u003e≥30μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTime per run\u003c\/td\u003e\n\u003ctd\u003eComplete 1-24 samples in 30 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLiquid carrying volume per column\u003c\/td\u003e\n\u003ctd\u003e800µl\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding yield of column\u003c\/td\u003e\n\u003ctd\u003e35µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch1\u003e\n\u003cbr\u003ePrinciple\u003c\/h1\u003e\n\u003cp\u003eThe HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method, then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).\u003c\/p\u003e\n\u003cp\u003eSee manual for more details on P1001:\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P1001_GBS-Manual_GB-Clean-Plasmid-Miniprep-Kit_Mar-2025_3c80698a-616e-483a-9356-c222c26eb340.pdf?v=1744374106\" target=\"_blank\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\" width=\"188\" height=\"53\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAdvantages\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.\u003c\/li\u003e\n\u003cli\u003eFast - it takes only 1 minute to obtain supernatant by optimized solution (10 minutes for other brands)\u003c\/li\u003e\n\u003cli\u003eHigh yield (see comparison below)- up to 35µg plasmid can be bound in one column\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ctable style=\"height: 209px;\" width=\"1185\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eContents\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003eP100101\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003eP100103\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003ePurification Times\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e50 Preps\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e250 Preps\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eRNase A\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e2 mg\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e10 mg\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer P1\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e15 ml\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e80 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer P2\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e15 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e80 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer P3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e20 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e100 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer PW1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e25 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e140 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eBuffer PW2*\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e10 ml\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e50 ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eElution Buffer\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e6 ml\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e30  ml\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003eHiPure DNA Mini Columns II\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003ctd style=\"height: 19px; width: 319.177px;\"\u003e2 ml Collection Tubes\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 98.9062px;\"\u003e50\u003c\/td\u003e\n\u003ctd style=\"height: 19px; width: 112.583px;\"\u003e250\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage and Stability\u003c\/h2\u003e\n\u003cp\u003eThe kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃\u003cspan\u003e \u003c\/span\u003eto dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.\u003c\/p\u003e\n\u003ch2\u003eExperiment Data\u003c\/h2\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/experimentaldatafigureforminiprepmagen.png?v=1681153639\" width=\"1026\" height=\"426\"\u003e\u003c\/p\u003e\n\u003cp\u003eSee also the attached data figure.\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/P1001_HiPure_Plasmid_DNA_Mini_Kit-GBS_1.pdf?v=1700841634\"\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Preps","offer_id":39766548119652,"sku":"P100101","price":74.94,"currency_code":"CAD","in_stock":true},{"title":"250 Preps","offer_id":39766548185188,"sku":"P100103","price":349.27,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/p100101_image_3.jpg?v=1742213763"},{"product_id":"marine-animal-tissue-genomic-dna-kit","title":"Marine Animal Tissue Genomic DNA Kit","description":"\u003cp\u003e\u003cspan\u003eThe Marine Animal Tissue Genomic DNA Purification Kit provides a simple and rapid method for high quality genomic DNA purification from tissues(either fresh or frozen at -70\u003cspan\u003e°\u003c\/span\u003eC until use) of marine animal, such as fish, shrimp, shellfish or crab. The Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol\/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-35 µg from 10 mg of tissue. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Features\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Efficient: 3-35 µg of genomic DNA from 10 mg of tissue or 1 x 106-107 cultrue cells. \u003cbr\u003e Fast: Procedure takes only 30 min. \u003cbr\u003e Universal: Purifies genomic DNA from various sources. \u003cbr\u003e Safe: No phenol\/cholroform extraction step. \u003cbr\u003e High purity: Purified DNA is ready for downstream application such as PCR, restriction digestion.\u003cbr\u003e\u003cbr\u003e Downstream Applications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Purified DNA is free from contaminants and enzyme inhibitors, and typically has A260\/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eü\u003c\/span\u003e\u003cspan\u003e Restriction digestion\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eü\u003c\/span\u003e\u003cspan\u003e PCR\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eü\u003c\/span\u003e\u003cspan\u003e Labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eü\u003c\/span\u003e\u003cspan\u003e Library construction\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eKit Content: (for a 50-prep kit size)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eComponent \u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eVolume or Size\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eSolution DS\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eSolution MS\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eProteinase K\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWash Buffer (PS)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e30 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWash Buffer (PE)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eElution Buffer TE\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e 5 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eSpin Columns\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50 each\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003cp\u003e\u003cspan\u003e\u003cbr\u003e\u003cbr\u003e Storage\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Store Protein K at -20\u003cspan\u003e℃\u003c\/span\u003e, other reagents can be stored at room temperature for up to 1 year. Any precipitate in the Solution DS and Solution MS can be re-dissolved by incubating at 37\u003cspan\u003e°\u003c\/span\u003eC before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e Important Notes\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Prior to the initial use of the kit, dilute the Wash Buffer(PE) with ethanol (96-100%):\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eN1172(100preps)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWash Buffer(PE)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e30 ml x2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eEthanol\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e, , 90 ml x2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eTotal Volume\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e120 ml x2\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAfter the ethanol has been added, mark the check box on the bottle to indicate the completed step.\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e Examine the solution for precipitates before each use. Re-dissolve any precipitate by warming the solution to 37\u003cspan\u003e°\u003c\/span\u003eC and cooling to 25\u003cspan\u003e°\u003c\/span\u003eC.\u003cbr\u003e\u003cspan\u003e•\u003c\/span\u003e All purification steps should be carried out at room temperature.\u003cbr\u003e\u003cbr\u003e Protocol\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1.Marine animal tissue (either fresh or frozen at -70\u003cspan\u003e°\u003c\/span\u003eC until use) can be processed by freezing with liquid nitrogen and grinding into a fine powder using a mortar and pestle. Add 10mg of this tissue powder to a 1.5ml microcentrifuge tube. Do not exceed 10 mg or it will reduce the lyse efficiency.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.Add 200 \u003cspan\u003eμ\u003c\/span\u003el Solution DS. Vortex vigorously to resuspend cells.It is essential that the sample and Solution DS are mixed immediately and thoroughly by vortexing or pipetting to yield a homogeneous solution. \u003cbr\u003e Optional If RNA-free genomic DNA is required, add 4 \u003cspan\u003eμ\u003c\/span\u003el RNase A (100 mg\/ml) and incubate for 5 minutes at room temperature. RNase A (100 mg\/ml) can be purchased separately .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3.Add 20 \u003cspan\u003eμ\u003c\/span\u003el Proteinase K. Mix thoroughly by vortexing. Incubate at 55\u003cspan\u003e℃\u003c\/span\u003e and inverting at times till a clear homogeneou solution is produced, spin down the water beads from the wall of the tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4.Add 220 \u003cspan\u003eμ\u003c\/span\u003el Solution MS, Mix thoroughly by vortexing. Incubate at 65\u003cspan\u003e°\u003c\/span\u003eC for 10 minutes to yield a homogeneous solution. Spin down the water beads from the wall of the tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5.Add 220 \u003cspan\u003eμ\u003c\/span\u003el ethanol (96\u003cspan\u003e–\u003c\/span\u003e100%) to the sample, and mix thoroughly by inverting. A precipitate may appear. Pipet the mixture from step 4 into the spin column placed in a 2 ml collection tube (provided). Centrifuge at 12,000rpm for 1 min. Discard flow-through.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6.Add 500 \u003cspan\u003eμ\u003c\/span\u003el Wash Buffer PS, and centrifuge for 1 min at 12,000 rpm. Discard flow-through.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7.Add 500 \u003cspan\u003eμ\u003c\/span\u003el Wash Buffer PE, and centrifuge for 1 min at 12,000 rpm. Discard flow-through. Repeat step 6 again.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e8.Centrifuge for 3 min at 12,000 rpm to dry the column membrane. Discard flow-through and collection tube. \u003cbr\u003e Note It is important to dry the membrane of the spin column, since residual ethanol may interfere with subsequent reactions. This centrifugation step ensures that no residual ethanol will be carried over during the following elution.Following the centrifugation step, remove the spin column carefully so that the column does not come into contact with the flow-through, since this will result in carryover of ethanol. If carryover of ethanol occurs, empty the collection tube, then reuse it in another centrifugation for 1 min at 12,000 rpm.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e9.Place the spin column in a clean 1.5 ml microcentrifuge tube (not provided), and pipet 30-100 \u003cspan\u003eμ\u003c\/span\u003el Eluent Buffer AE (prewarm to 65\u003cspan\u003e℃\u003c\/span\u003e)directly onto the membrane. Incubate at room temperature for 2 minutes, and then centrifuge for 2 min at 12,000 rpm to elute. The tube contains the purified DNA. Store the DNA at -20\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766548676708,"sku":"N117-01","price":109.74,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766548709476,"sku":"N117-02","price":208.5,"currency_code":"CAD","in_stock":true},{"title":"200 preps","offer_id":39766548742244,"sku":"N117-03","price":395.05,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/263.jpg?v=1643677387"},{"product_id":"gb-clone-t4-dna-ligase","title":"GB-Clone™ T4 DNA Ligase","description":"\u003cp\u003eThis enzyme is supplied in a buffer of 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 10 mM 2-mercaptoethanol, 0.1 mM EDTA and 50% glycerol. It was purified from an E. coli strain expressing a recombinant clone of T4 DNA ligase. Its molecular Weight is 68kDa.\u003c\/p\u003e\n\u003cp\u003eRequirements: Mg2+, ATP and DTT. The optimum concentration of Mg2+ is 10mM. Mn2+ may be\u003cbr\u003esubstituted for Mg2+ but is only 25% as effective as Mg2+.\u003cbr\u003eInhibition: 50% inhibition by greater than 150mM NaCl (activity measured at nicks. Other inhibitors\u003cbr\u003einclude 0.2M K+, Cs+, Li+, NH4+ and 1mM spermine.\u003cbr\u003eInactivation: Heat to 70°C for 10 minutes.\u003c\/p\u003e\n\u003cp\u003eContents: GB-Clone™ T4 DNA Ligase (200 U\/μl)\u003cbr\u003e10 X Ligation Buffer\u003c\/p\u003e\n\u003cp\u003eWe recommend that 0.5-1 μl of the ligase is used per 10 μl ligation reaction. \u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003eApplications\u003cbr\u003e Cloning of restriction fragments.\u003cbr\u003e Joining linkers and adapters to blunt-ended DNA\u003cbr\u003eUnit Definition\u003cbr\u003eOne unit of GB-Clone™ T4 DNA Ligase is defined as the amount of enzyme required to give 50%\u003cbr\u003eligation of Hind III fragments of λ DNA (5´ DNA termini concentration of 0.12 μM, 300-\u003cbr\u003eμg\/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1x T4 DNA Ligase\u003cbr\u003eReaction Buffer.\u003cbr\u003e\u003cbr\u003eStorage： Store at -20°C\u003cbr\u003eStorage Buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 200 μg\/ml BSA and 50% glycerol.\u003cbr\u003e10x T4 Ligase Buffer:\u003cbr\u003e660mM Tris-HCl(PH7.6), 66mM MgCl2, 100mM DTT, 10mM ATP\u003cbr\u003ePhysical Purity\u003cbr\u003eThe purity is ≥90% as judged by SDS-polyacrylamide gel electrophoresis with\u003cbr\u003eCoomassie® blue staining.\u003c\/p\u003e\n\u003cp\u003eStandard Applications\u003cbr\u003eWe recommend using a 1:1, 1:3 or 3:1 molar ratio of vector:insert DNA when cloning a\u003cbr\u003efragment into a plasmid vector. These ratios will vary with other types of vectors, for\u003cbr\u003eexample, cDNA and genomic cloning vectors. The following example illustrates the\u003cbr\u003econversion of molar ratios to mass ratios for a 3.0kb plasmid and a 0.5kb insert DNA\u003cbr\u003efragment.\u003c\/p\u003e\n\u003cp\u003eProtocol:\u003cbr\u003eThe following ligation reaction of a 3kb vector and a 0.5kb insert DNA uses a 1:1\u003cbr\u003evector:insert ratio. Typical ligation reactions use 100–200ng of vector DNA.\u003cbr\u003e1. Assemble the following reaction in a sterile microcentrifuge tube:\u003cbr\u003e10 X Ligation Buffer 1 μl\u003cbr\u003evector DNA 100ng\u003cbr\u003einsert DNA 1:1 to 10:1 molar ratio over vector\u003cbr\u003eT4 DNA Ligase 0.5–1μl\u003cbr\u003eNuclease-Free Water to final volume of 10μl\u003cbr\u003e2. Incubate the reaction at room temperature for 3 hours, or 16°C for 4-18 hours, or\u003cbr\u003e4 °C over night.\u003cbr\u003eNotes:\u003cbr\u003e1. Concatamers may form as ligation products. The extent of concatamer formation\u003cbr\u003edepends on the vector:insert ratio, incubation temperature and incubation time. This\u003cbr\u003eshould be taken into account when screening transformants.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/GB-Clone_T4_DNA_Lignase.pdf?v=1700752426\"\u003e\u003cspan\u003e\u003cimg data-mce-fragment=\"1\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"164\" height=\"46\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" data-mce-selected=\"1\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50uL","offer_id":39766548873316,"sku":"DNK4301-01","price":89.98,"currency_code":"CAD","in_stock":true},{"title":"200uL","offer_id":39766548906084,"sku":"DNK4302-02","price":301.77,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/264.jpg?v=1643677393"},{"product_id":"low-melting-lm-agarose","title":"Low-Melting (LM) Agarose","description":"\u003cp\u003e\u003cspan\u003eLow-Melting (LM) Agarose is \u003c\/span\u003e\u003cspan\u003e an high purity \u003c\/span\u003e\u003cb\u003eLow Melting\u003c\/b\u003e\u003cspan\u003ePoint \u003c\/span\u003e\u003cb\u003eAgarose\u003c\/b\u003e\u003cspan\u003e, which is ideal for resolving DNA fragments from 10 to 1,000 bp with a \u003c\/span\u003e\u003cb\u003elow melting\u003c\/b\u003e\u003cspan\u003e temperature of 65°C or less for easy recovery of DNA from agarose bands. \u003c\/span\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"10g","offer_id":39766549004388,"sku":"ELA-02-01","price":149.97,"currency_code":"CAD","in_stock":true},{"title":"25g","offer_id":39766549037156,"sku":"ELA-02-02","price":342.79,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_add83506-36d0-43a4-9706-9a0814148b15.jpg?v=1707315039"},{"product_id":"mieasy-microrna-kit","title":"miEASY microRNA Kit","description":"\u003cp\u003emiEASY microRNA Blood\/Serum\/Plasma Kit is a Blood microRNA extraction kit. Similar to Qiagen miRNeasy* Serum\/Plasma \/whole blood Kit. In addition to Serum and plasma, our miEASY kit can also be used to isolate miRNA from whole blood.\u003c\/p\u003e\n\u003cp\u003eFor purification of cell-free total RNA, including miRNA, from animal and human plasma and serum\u003c\/p\u003e\n\u003cp\u003e• Internal control available for normalization\u003c\/p\u003e\n\u003cp\u003e• Minimal elution volume\u003c\/p\u003e\n\u003cp\u003e• High-purity RNA suitable for all downstream applications\u003c\/p\u003e\n\u003cp\u003e• Easy, robust procedures\u003c\/p\u003e\n\u003cp\u003e• Automatable protocol\u003c\/p\u003e\n\u003cp\u003eThe MiEasy Serum\/Plasma Kit is designed for purification of cell-free total RNA — primarily miRNA and other small RNA — from small volumes of serum and plasma. RNA from serum and plasma typically consists of molecules \u0026lt;100 nucleotides.\u003c\/p\u003e\n\u003cp\u003e*miRNeasy is a name of similar product of Qiagen\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 Preps","offer_id":39766549299300,"sku":"RNK4601-01","price":274.33,"currency_code":"CAD","in_stock":true},{"title":"100 Preps","offer_id":39766549332068,"sku":"RNK4601-02","price":526.74,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/266.jpg?v=1643677408"},{"product_id":"le-agarose","title":"LE Agarose","description":"\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eGeneBio's LE agarose is a ultra pure agarose indicated for all your molecular biology agarose DNA electrophoresis experiments. \u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures include\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNuclease and protease-free\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eLow electroendosmosis (EEO) so you get a high quality gel each time\u003c\/li\u003e\n\u003cli\u003eGreat for analyzing your PCR products, plasmid preps, restriction digestion and other DNA fragment analyses\u003c\/li\u003e\n\u003cli\u003eHigh gel strength so that you can pick up and move your gels around without breakage\u003c\/li\u003e\n\u003cli\u003eLow in sulfide and other impurities. \u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Gene Bio Systems","offers":[{"title":"100g","offer_id":39766549430372,"sku":"ELA-03-01","price":131.03,"currency_code":"CAD","in_stock":true},{"title":"500g","offer_id":39766549463140,"sku":"ELA-03-02","price":539.24,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/agaroseimage2.png?v=1693241760"},{"product_id":"pax-blood-mirna-kit","title":"PAX Blood miRNA Kit","description":"\u003cp\u003ePAX Blood miRNA extraction Kit is used to extract miRNA form PAXgene blood tube. Kits of 50 preps are 100 preps are both available.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"50 preps","offer_id":39766549561444,"sku":"RNK5101-01","price":438.94,"currency_code":"CAD","in_stock":true},{"title":"100 preps","offer_id":39766549594212,"sku":"RNK5101-02","price":768.14,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/271.jpg?v=1643677419"},{"product_id":"on-column-dnase-digestion-kit","title":"On-Column DNase Digestion Kit","description":"\u003cp\u003e\u003cstrong\u003e50rxns\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eDNase Digestion Kit (On Spin Columns) is for removing DNA on spin columns used during RNA isolation procedures. Although these procedures are capable of removing most DNA, small amount of DNA remains. For demanding applications where all DNA must be removed, on-column digestion can be performed. Same as Qiagen RNase free DNase set (Cat# 79254*).\u003c\/p\u003e\n\u003cp\u003eSee protocol details for RNI02 in manual:\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/RNK3401_RNI02_Protocol_ON_Column_DNase_Digestion-_GBS_April_2025.pdf?v=1743602031\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"228\" height=\"64\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e*Note: this catalog number is that of a product of Qiagen.\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766549659748,"sku":"RNI02","price":108.63,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/1_194ad8f7-7c81-4ae4-af8a-50100cafdca9.jpg?v=1757609201"},{"product_id":"cold-sensitive-mutant-csm-taq-dna-pol","title":"Cold Sensitive Mutant (CSM) Taq DNA Pol","description":"\u003cp\u003e\u003cspan\u003eCSM Taq polymerases (Cold sensitive mutant Taq polymerases ) is a kind of HotStart Polymerase. Sourced from the mutant Ecoli. CSM Taq polymerase, unlike the monoclonal antibody based hot start Taq or chemical modified hot start Taq,Cold Taq is the cold sensitive mutant Taq. It retaisn the hot start ability throughout the whole amplification cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCold sensitive mutant Taq polymerases are designed for Hot Start PCR, it is not active at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq. It is designed for PCR with difficult templates such as GC-rich fragments and microsatellites. Cold sensitive mutant Taq polymerases are particularly well suited to primer extension of Single Nucleotide Polymorphism (SNP) markers.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCold sensitive mutant Taq polymerases maintain excellent specificity and minimal background even in conditions designed for high yield. In fact, even on genomic templates, the enzyme can be used with MgCl2+concentrations as high as 10 mM.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCold sensitive mutant Taq polymerases are capable of extending through difficult regions, e.g. regions, which include inverted tandem repeats and those with high amounts of secondary structure.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCold sensitive mutant Taq polymerases work in a totally unique way, involving improved nucleotide selection at the active site, and a much lower rate of mis-match extension, meaning that only perfectly aligned primers will be extended. As a result, the enzyme can give even higher specificity than hot-start (manual or automatic) techniques without the need for inconvenient pre-incubation steps.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eConc. 5 U\/μl Package: Bulk\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStore at -20°C\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1 Hot start PCR amplification\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2 Specific amplification of complex cDNA and genomic template, for amplification of difficult templates, such as GC-rich fragments and microsatellites\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3 Primer extension of SNP markers\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4 Amplification of genomic DNA targets up to 10 kb with high fidelity, specificity, and sensitivity\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5 Amplification from low copy number DNA template, high through-put Hot Start PCR with high specificity, sensitivity, and yield\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6 Routine diagnostic Hot Start PCR requiring high reproducibility.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7 Real-Time PCR\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e8 Multiple PCR\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e9 Generation of PCR products for TA cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eQuality Control:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFunctional absence of double and single-stranded endonuclease activity; Purity\u0026gt;99% test by SDS gel electrophoresis; Each lot of CSM Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA ; Retain full activity at room temperature for one week; No host DNA residue.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1000 U","offer_id":39766549921892,"sku":"DNK2101","price":109.74,"currency_code":"CAD","in_stock":true},{"title":"5000 U","offer_id":39766549954660,"sku":"DNK2101","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/274.jpg?v=1643677433"},{"product_id":"trans1-t1-phage-chemically-competent-cell","title":"Trans1-T1 Phage Chemically Competent Cell","description":"\u003ch3\u003e\u003cspan style=\"color: #ff2a00;\"\u003e\u003cstrong\u003eAdditional $40 shipping charge required for dry ice shipping\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cblockquote\u003e\n\u003cp\u003e\u003cstrong\u003eSupplied in 2 x 50μl  reactions per vial; 100μl per vial.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"text-decoration: underline;\"\u003e\u003cem\u003e20 reaction size is delivered in 10 vials; 40 reaction size is delivered in 20 vials. \u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003ci\u003eTrans1\u003c\/i\u003e-T1 Phage Resistant Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10\u003csup\u003e9\u003c\/sup\u003e cfu\/μg DNA (tested by pUC19 plasmid DNA).\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eGenotype\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eF-φ80(lacZ)ΔM15ΔlacX74hsdR(rk\u003c\/span\u003e\u003cspan\u003e-\u003c\/span\u003e\u003cspan\u003e, mk\u003c\/span\u003e\u003cspan\u003e+\u003c\/span\u003e\u003cspan\u003e)ΔrecA1398endA1tonA\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli class=\"p\"\u003e\n\u003cspan\u003eHigh transformation efficiency: \u0026gt;10\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e9\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e cfu\/μg (pUC19 DNA).\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\"\u003e\n\u003cspan\u003eFast-growing, colonies are visible in 8~9 hours.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\"\u003e\n\u003cspan\u003eResistance to T1 and T5 phage.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"p\"\u003e\u003cspan\u003eBlue\/white selection.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eCompetent Cell at -70°C for six months\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eNotes\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eHigher efficiency transformation can be achieved by transforming cells immediately following thawing.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eAvoid repeated thawing.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eGentle handling is required for the entire procedure.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eReferences:\u003c\/h2\u003e\n\u003ctable class=\"layui-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eLiterature\u003c\/th\u003e\n\u003cth\u003eJournal\u003c\/th\u003e\n\u003cth\u003eIF\u003c\/th\u003e\n\u003cth\u003eAuthor\u003c\/th\u003e\n\u003cth\u003eDate\u003c\/th\u003e\n\u003cth\u003eLink\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eRemoval of antibiotic resistance genes from wastewater treatment plant effluent by coagulation\u003c\/td\u003e\n\u003ctd\u003ewater research\u003c\/td\u003e\n\u003ctd\u003e6.9419\u003c\/td\u003e\n\u003ctd\u003eNa Li,et al.\u003c\/td\u003e\n\u003ctd\u003e2017Mar\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Removal+of+antibiotic+resistance+genes+from+wastewater+treatment+plant+effluent+by+coagulation\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduction and characterization of poly(3-hydroxypropionate-co-4-hydroxybutyrate) with fully controllable structures by recombinant Escherichia coli containing an engineered pathway\u003c\/td\u003e\n\u003ctd\u003eMetabolic Engineering\u003c\/td\u003e\n\u003ctd\u003e6.859\u003c\/td\u003e\n\u003ctd\u003eMeng DC, et al. MOE Key Lab of Bioinformatics, Dep\u003c\/td\u003e\n\u003ctd\u003e2012 Jul\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22561235\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAbsolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification\u003c\/td\u003e\n\u003ctd\u003eanalytical chemistry\u003c\/td\u003e\n\u003ctd\u003e6.32\u003c\/td\u003e\n\u003ctd\u003eYi Hu,et al.\u003c\/td\u003e\n\u003ctd\u003e2016Nov\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Absolute+Quantification+of+H5-subtype+Avian+Influenza+Viruses+Using+Droplet+Digital+Loop-mediated+Isothermal+Amplification\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 years\u003c\/td\u003e\n\u003ctd\u003ejournal of hazardous materials\u003c\/td\u003e\n\u003ctd\u003e6.065\u003c\/td\u003e\n\u003ctd\u003eShuang Peng,et al.\u003c\/td\u003e\n\u003ctd\u003e2017Qct\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Prevalence+of+antibiotic+resistance+genes+in+soils+after+continually+applied+with+different+animal+manure+for+30+years\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEffect of fumigation with chloropicrin on soil bacterial communities and genes encoding key enzymes involved in nitrogen cycling\u003c\/td\u003e\n\u003ctd\u003eEnvironmental Pollution\u003c\/td\u003e\n\u003ctd\u003e5.0989\u003c\/td\u003e\n\u003ctd\u003eJun Li,et al.\u003c\/td\u003e\n\u003ctd\u003e2017Aug\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Effect+of+fumigation+with+chloropicrin+on+soil+bacterial+communities+and+genes+encoding+key+enzymes+involved+in+nitrogen+cycling\"\u003eThe original link\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"10 reactions","offer_id":39766550052964,"sku":"CD501-01","price":182.84,"currency_code":"CAD","in_stock":true},{"title":"20 reactions","offer_id":39766550085732,"sku":"CD501-02","price":332.65,"currency_code":"CAD","in_stock":true},{"title":"40 reactions","offer_id":39766550118500,"sku":"CD501-03","price":626.7,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/275.jpg?v=1643677438"},{"product_id":"peasy-r-blunt3-cloning-kit","title":"pEASY®-Blunt3 Cloning Kit","description":"\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cem\u003e\u003cspan\u003epEASY\u003c\/span\u003e\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e®\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e•5 minutes fast ligation of Pfu-amplified PCR products.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e•Ampicillin resistance gene for selection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e•Easy blue\/white selection.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e•T7 promoter and SP6 promoter for in vitro transcription.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cem\u003e\u003cspan\u003eTrans1\u003c\/span\u003e\u003c\/em\u003e\u003cspan\u003e-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (\u0026gt;109 cfu\/μg pUC19 DNA) and fast growing.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eCompetent Cell at -70℃ for six months; others at -20℃ for 9 months\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eKit Content\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eComponent\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eCB301-01\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e\u003cspan\u003eCB301-02\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e(20 rxns)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e(60 rxns)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cem\u003e\u003cspan\u003epEASY \u003c\/span\u003e\u003c\/em\u003e\u003cspan\u003e\u003cspan\u003e®\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e-Blunt3 Cloning Vector (10 ng\/μl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e20\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e3×20 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eControl Template (5 ng\/μl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eControl Primers (10 μM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eM13 Forward Primer (10 μM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e150\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eM13 Reverse Primer (10 μM)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e50\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e150\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eµl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003e\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003ePlease note: this kit does not include competent cells. \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Gene Bio Systems","offers":[{"title":"20 reactions","offer_id":39766550216804,"sku":"CB301","price":324.81,"currency_code":"CAD","in_stock":true},{"title":"60 reactions","offer_id":39766550249572,"sku":"CB301","price":763.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/284.jpg?v=1643677442"},{"product_id":"purair-pcr-series-workstations-dead-air-models","title":"PURAIR-PCR SERIES WORKSTATIONS (Dead Air models)","description":"\u003cdiv\u003e\n\u003cp\u003e\u003cem\u003e\u003cspan data-olk-copy-source=\"MessageBody\"\u003eThis price is a signal that a quotation must be obtained before placing an order. Please reach out to us for a quote\u003c\/span\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003ch2\u003ePurair PCR Workstations\u003c\/h2\u003e\n\u003ch2\u003e-Features and Benefits\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003ch3\u003eConvenience and Construction Benefits\u003c\/h3\u003e\n\u003cp\u003eClick to view the\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/Purair_PCR_Brochure.pdf?v=1684164469\"\u003e brochure \u003c\/a\u003efor more information\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePurair PCR dead-air cabinets\/PCR workstations\/PCR hoods are available in three model sizes with various options.\u003c\/li\u003e\n\u003cli\u003eWorkstations\/Cabinets are shipped fully assembled with standard power cords; no installation required.\u003c\/li\u003e\n\u003cli\u003eThe integral UV lamp fixtures are pre-installed.\u003c\/li\u003e\n\u003cli\u003eAll PCR workstation\/ hood filters are pre-installed, easy to access and easy to change.\u003c\/li\u003e\n\u003cli\u003ePCR dead air workstations products are designed for desktop use, or may be installed on an optional base (extra charge) stand or mobile cart (not supplied).\u003c\/li\u003e\n\u003cli\u003ePurair PCR workstations (dead air hoods) are constructed of steel with a microbial powder finish coating.\u003c\/li\u003e\n\u003cli\u003eThe optional stainless steel work surface includes lips on all four sides to contain spills.\u003c\/li\u003e\n\u003cli\u003eCSA-certified\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch3\u003eFeatures:\u003c\/h3\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eA. Task Lighting:\u003c\/span\u003e Fluorescent cabinet lamp located away from laminar flow area.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eB. Ultraviolet Lighting:\u003c\/span\u003e Built-in ultraviolet lamp with timer permits decontamination between PCR cycles. Includes a double-flap safety cover of 0.2\" (5 mm) UV absorbing 254 nm beta radiation resistant polycarbonate; provides superior operator protection while allowing easy access to the work zone. Timer can be set over range of 0 to 60 minutes or set to HOLD.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e\u003cspan\u003eC. Comfort:\u003c\/span\u003e\u003cspan\u003e Ergonomically angled front improves reach and user comfort.\u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eD. Decontamination Shelf:\u003c\/span\u003e Mounted on the back wall is proximate to the UV lamp for maximum exposure.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eF. Pass Through Ports:\u003c\/span\u003e Convenient rear-wall pass through ports for safe routing of instrument cords, cables and leads.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eG. Double-Hinged Self-Locking Front Sash:\u003c\/span\u003e With overlap closed, the cabinet sash protects samples on the work surface from contamination with 100 fpm airflow. The sash completely closes unit off when not in use and is easy to open and latch.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cimg alt=\"PCR ductless fume hood callouts\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/t\/6\/assets\/Puair-PCR-ductless-workstation-features.jpg?v=1643677451\"\u003e\n\u003cdiv class=\"caption\"\u003ePurair PCR-24, shown with optional mobile cart and optional stainless steel work surface.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eI. Stand:\u003c\/span\u003e Optional base stand converts to mobile cart with optional locking casters.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eJ. Work Surface:\u003c\/span\u003e Large polypropylene work surface, white or black standard, with optional stainless steel work surface available.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eK. Magnetic Door Latch:\u003c\/span\u003e Safety interlock prevents accidental operator exposure when door is opened.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eOTHER FEATURES:\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003e360 Degree Visibility:\u003c\/span\u003e Clear back and side panels\u003c\/div\u003e\n\u003cdiv\u003eallow ambient illumination into the chamber and provide users with an unobstructed view of its contents.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eStandards Compliant:\u003c\/span\u003e Performance specifications and construction meet or exceed OSHA, ANSI and relevant international standards to assure operator safety.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eConstruction:\u003c\/span\u003e All models are available in either metal or polypropylene construction, specify metal or polypropylene when ordering. Available in 120V, 60Hz or 220V, 50Hz models.\u003c\/div\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cspan\u003eSteel Support Frame:\u003c\/span\u003e The chemical resistant epoxy coated steel frame adds mechanical strength. Optional all polypropylene construction is available if desired.\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003eatured Purair PCR Laminar Flow Models\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"featured_products\"\u003e\n\u003cdiv class=\"product\"\u003e\n\u003cimg alt=\"Purair PCR Laminar Flow Cabinet\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/t\/6\/assets\/PCR-24-Purair-PCR-laminar-flow-cabinet2x.jpg?v=1643677453\" width=\"78\" height=\"100\"\u003e\n\u003ch4\u003ePCR-24\u003c\/h4\u003e\n\u003cp\u003e24\"W\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product\"\u003e\n\u003cimg alt=\"Purair PCR Laminar Flow Cabinet\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/t\/6\/assets\/PCR-36-Purair-PCR-laminar-flow-cabinet2x.jpg?v=1643677454\" width=\"112\" height=\"100\"\u003e\n\u003ch4\u003ePCR-36\u003c\/h4\u003e\n\u003cp\u003e36\"W\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product\"\u003e\n\u003cimg alt=\"Purair PCR Laminar Flow Cabinet\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/t\/6\/assets\/PCR-48-Purair-PCR-front2x.jpg?v=1643677456\" width=\"120\" height=\"100\"\u003e\n\u003ch4\u003ePCR-48\u003c\/h4\u003e\n\u003cp\u003e48\"W\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003e\u003cbr\u003ePURAIR-PCR CUSTOM DEAD AIR BOX\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDead air box provides an enclosed work area with chemical resistant work surface. \u003cspan\u003econstruction: \u003cstrong\u003e\u003cspan\u003ewhite powder-coated electro-galvanized steel\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e with \u003cstrong\u003e\u003cspan\u003ePolycarbonate double-flap front cover \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eprovides safety to the operator. Includes fluorescent lighting and cable pass through ports.\u003cspan\u003e \u003c\/span\u003eAll electrical components are \u003cstrong\u003e\u003cspan\u003eUL-approved or recognized\u003c\/span\u003e\u003c\/strong\u003e\u003c\/span\u003e, All Units are 110-130VAC 60HZ or 230VAC 50HZ.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eOPTIONAL EQUIPMENT:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003ch4\u003e\u003cspan\u003eOPTION\u003c\/span\u003e\u003c\/h4\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePCR-24\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePCR-36\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePCR-48\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eGPR chemical resistant spillage tray\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eNo charge\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eNo charge\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eNo charge\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eUV light with dial timer and safety switch\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eIncluded\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eIncluded\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eIncluded\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eStainless Steel spillage tray\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e$350\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e$390\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e$450\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eAny question on PCR workstations, please talk to us first.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"PCR-24 dead air box (2ft wide)","offer_id":39766550446180,"sku":"PCR-24D,PCR-36D, PCR-48D","price":1.21,"currency_code":"CAD","in_stock":true},{"title":"PCR-36 dead air box (3 ft wide)","offer_id":39766550478948,"sku":"PCR-24D,PCR-36D, PCR-48D","price":1.21,"currency_code":"CAD","in_stock":true},{"title":"PCR-48 dead air box (4 ft 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