{"title":"Long PCR","description":"\u003cp\u003e\u003cspan style=\"color: #fbe329;\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan style=\"color: #000000;\"\u003eLong PCR is PCR of long or large ragments, often also referred to as long-range PCR. In order to perform long PCR, not only the cycling times need to be increased, but also the polymerases need to be modified. Specific formulations with Taq DNA polymerase spiked with a low amount of proof-reading enzymes such as Pfu DNA polymerases are often used (W M Barnes (1994) Proc Natl Acad Sci U S A. 91(6): 2216–2220.)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e \u003c\/span\u003e\u003c\/p\u003e","products":[{"product_id":"long-pcr-taq-master-mix-with-optimizer","title":"Long PCR Taq Master Mix with Optimizer","description":"\u003cp\u003e\u003cspan\u003eThis product is a 2X Long PCR reagent Mix, plus a separately supplied Long PCR DNA Polymerase. The mix contains dNTPs, Mg2+ and Buffer at optimal concentrations. This Optimzer format provides flexibility of optimizing Long DNA polymerase activity for each unique reaction and still the convenience that comes with master mixes. The Long Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex targets, such as GC-rich targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eLong PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, \u003c\/span\u003ewhich can be used in TA clone\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e 5 x 1 ml is supplied. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR amplification of DNA fragments any sizes around 5 kb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR for cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High fidelity: three times fidelity of Taq DNA Polymerase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Longer fragment: amplify long templates as long as 40kb.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Amplification of complex template (GC rich or repetitive sequence).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Generates 3'-dA and blunt end PCR products. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10 x Long PCR Buffer is classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10 x Long PCR Buffer \u003cspan\u003eⅡ\u003c\/span\u003e is an alternative long PCR buffer . It is for better fidelity but may not be robust for longer templates above 10 kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNotes on cycling conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e- Initial denaturation can be performed over an interval of 1~5 min at 95\u003cspan\u003e℃\u003c\/span\u003e depending on the GC content of template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Denaturation for 30 sec to 2 min at 94~95\u003cspan\u003e℃\u003c\/span\u003e is sufficient. If the amplified DNA has a very high GC content, denaturation time may be increased up to 4 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Optimal annealing temperature is 5\u003cspan\u003e℃\u003c\/span\u003e lower than the melting temperature of primer-temperature DNA duplex. If nonspecific PCR products are obtained optimization of annealing temperature can be performed by increasing temperature stepwise by 2\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The number of PCR cycles depends on the amount of emplate DNA in the reaction mix and on the expected yield of the PCR products, 25-35 cycles are usually sufficient \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003efor the majority PCR reaction. Low amounts of starting template may require 40 cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The time of the final extensi, on step can be extended for amplicons that will be cloned into T\/A vectors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"Default Title","offer_id":39766538748004,"sku":"P306","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/229.jpg?v=1643677180"},{"product_id":"long-pcr-taq-polymerase-master-mix","title":"Long PCR Taq polymerase Master Mix","description":"\u003cp\u003e\u003cspan\u003eMaster mix Long PCR Taq DNA Polymerase, the same high-performing DNA polymerases, now in the master mix format to provide you with convenience of use. The master mix is a special formulation containing the Long PCR Taq DNA polymerase. The Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex targets, such as GC-rich targets. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eJust add primers and template, your PCR reaction is ready to begin!\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, \u003c\/span\u003ewhich can be used in TA clone\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR amplification of DNA fragments any sizes around 5 kb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR for cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High fidelity: three times fidelity of Taq DNA Polymerase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Longer fragment: amplify long templates as long as 40kb.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Amplification of complex template (GC rich or repetitive sequence).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Generates 3'-dA and blunt end PCR products. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer\u003cspan\u003eⅠ\u003c\/span\u003eis classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer \u003cspan\u003eⅡ\u003c\/span\u003e is an alternatie long PCR buffer . It is for better fidelity but may not be robust for longer templates above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eBasic PCR Protocol\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following basic protocol serves as a general guideline and a starting point for any PCR amplification. Optimal reaction conditions \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(incubation time and temperature, concentration of Taq DNA Polymerase, primers, Mg2+, and template DNA) vary and need to be optimized. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNotes on cycling conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e- Initial denaturation can be performed over an interval of 1~5 min at 95\u003cspan\u003e℃\u003c\/span\u003e depending on the GC content of template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Denaturation for 30 sec to 2 min at 94~95\u003cspan\u003e℃\u003c\/span\u003e is sufficient. If the amplified DNA has a very high GC content, denaturation time may be increased up to 4 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Optimal annealing temperature is 5\u003cspan\u003e℃\u003c\/span\u003e lower than the melting temperature of primer-temperature DNA duplex. If nonspecific PCR products are obtained optimization of annealing temperature can be performed by increasing temperature stepwise by 2\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The number of PCR cycles depends on the amount of emplate DNA in the reaction mix and on the expected yield of the PCR products, 25-35 cycles are usually sufficient \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003efor the majority PCR reaction. Low amounts of starting template may require 40 cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The time of the final extensi, on step can be extended for amplicons that will be cloned into T\/A vectors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL","offer_id":39766544351332,"sku":"DNK300P-01","price":76.81,"currency_code":"CAD","in_stock":true},{"title":"3mL","offer_id":39766544416868,"sku":"DNK300P-02","price":197.52,"currency_code":"CAD","in_stock":true},{"title":"6mL","offer_id":39766544449636,"sku":"DNK300P-03","price":329.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/245.jpg?v=1643677287"},{"product_id":"long-pcr-taq-dna-polymerase","title":"Long PCR Taq DNA polymerase","description":"\u003cp\u003e\u003cspan\u003eLong PCR Taq DNA Polymerase,a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragment. This specially formulated Long PCR Taq was shown to amplify long templates from λ phage genome of up to 20 kb. It is also a better choice for amplifying complex template, such as GC-rich template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long PCR Taq in your PCR reactions results in 3´-dA overhangs PCR products, \u003c\/span\u003ewhich can be used in TA clone\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eContents: Long PCR Taq DNA Polymerase, PCR Enhancer, 6x gel loading buffer, 10X Long PCR Taq Buffer \u003cspan\u003eⅠ\u003c\/span\u003e with Mg2+, 10X Long PCR Taq Buffer \u003cspan\u003eⅡ\u003c\/span\u003e with Mg2+\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR amplification of DNA fragments any sizes around 5 kb\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA labeling\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• DNA sequencing\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• PCR for cloning\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eUnit Definition\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eOne unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP’s into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eStorage Buffer \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e20mM TrisCl ( pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT\u003cspan\u003e，\u003c\/span\u003e0.1% NP-40, 0.1% Tween20, 0.2mg\/ml BSA, 50% (v\/v) glycerol\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e10X Long PCR Taq Buffer \u003cspan\u003eⅠ\u003c\/span\u003e with Mg2+\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e500mM Tris-HCl pH 8.8\u003cspan\u003e，\u003c\/span\u003e160mM (NH4)2SO4 \u003cspan\u003e，\u003c\/span\u003e25mM MgCl2 \u003cspan\u003e，\u003c\/span\u003e1% Triton X-100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e10X Long PCR Taq Buffer \u003cspan\u003eⅡ\u003c\/span\u003e with Mg2+\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e200mM Tris-HCl PH8.8\u003cspan\u003e，\u003c\/span\u003e100mM KCl\u003cspan\u003e，\u003c\/span\u003e100mM (NH4)2SO4\u003cspan\u003e，\u003c\/span\u003e16mM MgSO4\u003cspan\u003e，\u003c\/span\u003e1% Tritonx-100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• High fidelity: three times fidelity of Taq DNA Polymerase.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Longer fragment: amplify long templates as long as 40kb.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Amplification of complex template (GC rich or repetitive sequence).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Generates 3'-dA and blunt end PCR products. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNote:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer\u003cspan\u003eⅠ\u003c\/span\u003eis classical Long PCR Taq DNA Polymerase buffer, is good for long template especially above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003cspan\u003e 10xLong PCR Buffer \u003cspan\u003eⅡ\u003c\/span\u003e is an alternatie long PCR buffer . It is for better fidelity but may not be robust for longer templates above 10kb.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003e• Users may choose compare the two buffers for different template. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e Basic PCR Protocol \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe following basic protocol serves as a general guideline and a starting point for any PCR amplification. Optimal reaction conditions \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(incubation time and temperature, concentration of Taq DNA Polymerase, primers, Mg2+, and template DNA) vary and need to be optimized. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e 1. Add the following components to a sterile microcentrifuge tube sitting on ice:\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eReagent\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVolume (50 µl rxn)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFinal concentration\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10x PCR Buffer\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e5 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1x\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003edNTPs(10 mM each)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.2 mM each\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer I\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.4-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer II\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.4-1 µM\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eLong PCR DNA polymerase (5U\/µl)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e0.25-0.5 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e1.25-2.5U\/50 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eWater\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eVariable to 50 µl\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eN.A.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. Mix contents of tube. Cap tubes and centrifuge briefly to collect the contents to the bottom. When using a thermal cycler that does not\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e contain a heated lid, overlay the reaction mixture with 25 μl mineral oil. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Perform 25-35 cycles of PCR amplification as follows:\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eInitial Denaturation\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e94°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e3 min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e25-35 Cycles\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e94°C \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e55-68°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e72°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e30s\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e30s\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e1-10 mins\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eFinal Extension\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e72°C \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e10 min\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan\u003e4. Incubate for an additional 10 min at 72°C and maintain the reaction at 4°C. The samples can be stored at -20°C until use. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Analyze the amplification products by agarose gel electrophoresis and visualize by ethidium bromide staining. Use appropriate molecular weigh standards.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eNotes on cycling conditions\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e- Initial denaturation can be performed over an interval of 1~5 min at 95\u003cspan\u003e℃\u003c\/span\u003e depending on the GC content of template.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Denaturation for 30 sec to 2 min at 94~95\u003cspan\u003e℃\u003c\/span\u003e is sufficient. If the amplified DNA has a very high GC content, denaturation time may be increased up to 4 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-Optimal annealing temperature is 5\u003cspan\u003e℃\u003c\/span\u003e lower than the melting temperature of primer-temperature DNA duplex. If nonspecific PCR products are obtained optimization of annealing temperature can be performed by increasing temperature stepwise by 2\u003cspan\u003e℃\u003c\/span\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The number of PCR cycles depends on the amount of emplate DNA in the reaction mix and on the expected yield of the PCR products, 25-35 cycles are usually sufficient \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003efor the majority PCR reaction. Low amounts of starting template may require 40 cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e-The time of the final extensi, on step can be extended for amplicons that will be cloned into T\/A vectors.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"250U","offer_id":39766556278884,"sku":"P1061","price":101.61,"currency_code":"CAD","in_stock":true},{"title":"1000U","offer_id":39766556311652,"sku":"P1063","price":355.62,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/324.jpg?v=1643677684"},{"product_id":"2-phanta-max-master-mix","title":"2X Phanta Max Master Mix","description":"\u003cp\u003e \u003c\/p\u003e\n\u003ch2 data-start=\"111\" data-end=\"145\"\u003e2× Phanta Max Master Mix\u003c\/h2\u003e\n\u003cp data-start=\"146\" data-end=\"191\" class=\"\"\u003e\u003cstrong data-start=\"146\" data-end=\"191\"\u003eUltra-High Fidelity. Supreme Performance.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-start=\"193\" data-end=\"607\" class=\"\"\u003e\u003cstrong data-start=\"193\" data-end=\"221\"\u003e2× Phanta Max Master Mix\u003c\/strong\u003e is a high-performance PCR solution featuring \u003cstrong data-start=\"267\" data-end=\"311\"\u003ePhanta Max Super-Fidelity DNA Polymerase\u003c\/strong\u003e, optimized for long-fragment amplification, superior specificity, and exceptionally low error rates. Thanks to its unique \u003cstrong data-start=\"434\" data-end=\"454\"\u003eextension factor\u003c\/strong\u003e, \u003cstrong data-start=\"456\" data-end=\"489\"\u003especificity-promoting factors\u003c\/strong\u003e, and \u003cstrong data-start=\"495\" data-end=\"524\"\u003eplateau-inhibiting factor\u003c\/strong\u003e, Phanta Max delivers unmatched fidelity and yield, even for challenging templates.\u003c\/p\u003e\n\u003cp data-start=\"609\" data-end=\"927\" class=\"\"\u003eCapable of amplifying fragments up to \u003cstrong data-start=\"647\" data-end=\"664\"\u003e40 kb (λ DNA)\u003c\/strong\u003e, \u003cstrong data-start=\"666\" data-end=\"689\"\u003e20 kb (genomic DNA)\u003c\/strong\u003e, and \u003cstrong data-start=\"695\" data-end=\"711\"\u003e10 kb (cDNA)\u003c\/strong\u003e, it’s an excellent choice for demanding applications. With an error rate \u003cstrong data-start=\"785\" data-end=\"807\"\u003e53x lower than Taq\u003c\/strong\u003e and \u003cstrong data-start=\"812\" data-end=\"833\"\u003e6x lower than Pfu\u003c\/strong\u003e, Phanta Max ensures high accuracy for cloning, sequencing, and other downstream applications.\u003c\/p\u003e\n\u003ch2 data-start=\"934\" data-end=\"956\"\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"959\" data-end=\"982\"\u003eUltra-High Fidelity\u003c\/strong\u003e – Ideal for accurate amplification and cloning\u003cbr data-start=\"1029\" data-end=\"1032\"\u003e\u003cstrong data-start=\"1034\" data-end=\"1069\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1034\" data-end=\"1069\"\u003eExceptional Amplification Range\u003c\/strong\u003e – 40 kb λ DNA, 20 kb genomic DNA, 10 kb cDNA\u003cbr data-start=\"1114\" data-end=\"1117\"\u003e\u003cstrong data-start=\"1119\" data-end=\"1150\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1119\" data-end=\"1150\"\u003eStrong Inhibitor Resistance\u003c\/strong\u003e – Direct PCR from bacteria, fungi, plant tissue, animal tissue, and even whole blood\u003cbr data-start=\"1235\" data-end=\"1238\"\u003e\u003cstrong data-start=\"1240\" data-end=\"1264\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1240\" data-end=\"1264\"\u003eHot-Start Technology\u003c\/strong\u003e – Dual monoclonal antibodies inhibit polymerase and exonuclease activity at room temperature\u003cbr data-start=\"1357\" data-end=\"1360\"\u003e\u003cstrong data-start=\"1362\" data-end=\"1384\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1362\" data-end=\"1384\"\u003eBlunt-End Products\u003c\/strong\u003e – Perfect for cloning with \u003cstrong data-start=\"1412\" data-end=\"1452\"\u003eClonExpress II One Step Cloning Kits\u003c\/strong\u003e (Cat. \u003ca href=\"https:\/\/www.genebiosystems.com\/products\/clonexpress-ii-one-step-cloning-kit\" rel=\"noopener\" target=\"_blank\"\u003e#C112\u003c\/a\u003e, #\u003ca href=\"https:\/\/www.genebiosystems.com\/products\/clonexpress-multis-one-step-cloning-kit\" rel=\"noopener\" target=\"_blank\"\u003eC113\u003c\/a\u003e)\u003cbr data-start=\"1472\" data-end=\"1475\"\u003e\u003cstrong data-start=\"1477\" data-end=\"1506\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1477\" data-end=\"1506\"\u003eConvenient \u0026amp; Reproducible\u003c\/strong\u003e – Just add primers and template; optimized for consistent performance\u003cbr data-start=\"1576\" data-end=\"1579\"\u003e\u003cstrong data-start=\"1581\" data-end=\"1603\"\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"957\" data-end=\"1666\"\u003e\n\u003cstrong data-start=\"1581\" data-end=\"1603\"\u003eFreeze-Thaw Stable\u003c\/strong\u003e – Protective formulation resists multiple freeze-thaw cycles\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1673\" data-end=\"1702\"\u003eIncluded in the Mix\u003c\/h2\u003e\n\u003cul data-start=\"1703\" data-end=\"1787\"\u003e\n\u003cli data-start=\"1703\" data-end=\"1747\" class=\"\"\u003e\n\u003cp data-start=\"1705\" data-end=\"1747\" class=\"\"\u003ePhanta Max Super-Fidelity DNA Polymerase\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1748\" data-end=\"1757\" class=\"\"\u003e\n\u003cp data-start=\"1750\" data-end=\"1757\" class=\"\"\u003edNTPs\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1748\" data-end=\"1757\" class=\"\"\u003e\n\u003cp data-start=\"1750\" data-end=\"1757\" class=\"\"\u003eOptimized reaction buffer\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-start=\"1794\" data-end=\"1811\"\u003eStorage\u003c\/h2\u003e\n\u003cp data-start=\"1812\" data-end=\"1872\" class=\"\"\u003e❄ \u003cstrong data-start=\"1814\" data-end=\"1832\"\u003eStore at –20°C\u003c\/strong\u003e for maximum stability and performance\u003c\/p\u003e","brand":"Gene Bio Systems","offers":[{"title":"1mL","offer_id":39771527872612,"sku":"P505-01","price":139.71,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":39771527807076,"sku":"P505-02","price":508.03,"currency_code":"CAD","in_stock":true},{"title":"15mL","offer_id":39771527839844,"sku":"P505-03","price":1270.08,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/741.jpg?v=1643777949"},{"product_id":"transstart-r-fastpfu-fly-dna-polymerase","title":"TransStart® FastPfu Fly DNA Polymerase","description":"\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cem\u003eTransStart\u003c\/em\u003e®\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eFastPfu\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eFly DNA Polymerase is a hot start, high-fidelity and high processivity DNA Polymerase used for fast PCR. Compared with\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTransStart\u003c\/em\u003e®\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eFastPfu\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eDNA Polymerase,\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTransStart\u003c\/em\u003e®\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eFastPfu\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eFly DNA Polymerase has higher extension rate (6kb\/min vs 4kb\/min), featuring higher amplification efficiency, higher yield, higher fidelity and higher specificity.2×\u003cem\u003eTransStart\u003c\/em\u003e® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water and\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eTransStart\u003c\/em\u003e®\u003cem\u003e\u003cspan\u003e \u003c\/span\u003eFastPfu\u003cspan\u003e \u003c\/span\u003e\u003c\/em\u003eFly DNA Polymerase. The Reaction can be carried out with the concentration of Reaction Mix being 1×. PCR products are blunt end and can be cloned directly into\u003cspan\u003e \u003c\/span\u003e\u003cem\u003epEASY\u003c\/em\u003e®-Blunt series of vectors.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e• Offers 108-fold fidelity as compared to\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eEasyTaq\u003c\/em\u003e® DNA Polymerase.\u003c\/p\u003e\n\u003cp\u003e• PCR products can be directly cloned into\u003cspan\u003e \u003c\/span\u003e\u003cem\u003epEASY\u003c\/em\u003e®-Blunt vectors. \u003c\/p\u003e\n\u003cp\u003e• Amplification of genomic DNA fragment up to 15 kb. \u003c\/p\u003e\n\u003cp\u003e• Amplification of plasmid DNA fragment up to 20 kb.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e• Hot start, high specificity.\u003c\/p\u003e\n\u003cp\u003e• High amplification efficiency.\u003c\/p\u003e\n\u003cp\u003e• Fast and high fidelity.\u003c\/p\u003e\n\u003cp\u003e• High sensitivity.\u003c\/p\u003e\n\u003cp\u003e• High production.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003cbr\u003eat -20 ℃ for two years\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping\u003c\/strong\u003e\u003cbr\u003eDry ice (-70 ℃)\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cdiv class=\"clear\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eProduct Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ctable border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"firstRow\"\u003e\n\u003ctd\u003eComponent\u003c\/td\u003e\n\u003ctd\u003eAP231-21-V3\u003c\/td\u003e\n\u003ctd\u003eAP231-22-V3\u003c\/td\u003e\n\u003ctd\u003eAP231-23-V3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eTransStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cem\u003eFastPfu\u003c\/em\u003e\u003cspan\u003e \u003c\/span\u003eFly DNA Polymerase\u003c\/td\u003e\n\u003ctd\u003e250 U×1\u003c\/td\u003e\n\u003ctd\u003e500 U×1\u003c\/td\u003e\n\u003ctd\u003e500 U×6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2×\u003cem\u003eTransStart\u003c\/em\u003e\u003csup\u003e®\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003eFastPfu Fly Reaction\u003c\/td\u003e\n\u003ctd\u003e1 ml×3\u003c\/td\u003e\n\u003ctd\u003e1 ml ×6\u003c\/td\u003e\n\u003ctd\u003e1 ml ×36\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50 mM MgSO\u003csub\u003e4\u003c\/sub\u003e\n\u003c\/td\u003e\n\u003ctd\u003e200 μl×1\u003c\/td\u003e\n\u003ctd\u003e400 μl ×1\u003c\/td\u003e\n\u003ctd\u003e1 ml ×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePCR Stimulant\u003c\/td\u003e\n\u003ctd\u003e200 μl×1\u003c\/td\u003e\n\u003ctd\u003e400 μl×1\u003c\/td\u003e\n\u003ctd\u003e1 ml ×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e6×DNA Loading Buffer\u003c\/td\u003e\n\u003ctd\u003e500 μl×1\u003c\/td\u003e\n\u003ctd\u003e1 ml ×1\u003c\/td\u003e\n\u003ctd\u003e1 ml ×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cdiv class=\"clear\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/AP231_2023-03-05-GBS.pdf?v=1700584260\"\u003e\u003cimg height=\"43\" width=\"153\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cspan class=\"author\"\u003e\u003cstrong\u003eCitations\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"acontentc\"\u003e\n\u003cp\u003e\u003cspan\u003eZhu X J , Sun S , Xie B , \u003cem\u003eet al\u003c\/em\u003e. Guanine-rich sequences inhibit proofreading DNA polymerases[J]. Scientific Reports, 2016, 6:28769.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eYe F , Lan X E , Zhu W B , \u003cem\u003eet al\u003c\/em\u003e. Mitochondrial genomes of praying mantises (Dictyoptera, Mantodea): rearrangement, duplication, and reassignment of tRNA genes[J]. Scientific Reports, 2016, 6:25634.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eHu L , Li H , Qin R ,\u003cem\u003e et al\u003c\/em\u003e. Plant phosphomannose isomerase as a selectable marker for rice transformation[J]. Scientific Reports, 2016, 6(1):25921.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eChao H , Zhou N Y . GenR, an IclR-Type Regulator, Activates and Represses the Transcription of gen Genes Involved in 3-Hydroxybenzoate and Gentisate Catabolism in Corynebacterium glutamicum[J]. Journal of Bacteriology, 2013, 195(7):1598-1609.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eLiu X, Pu J, Zeng S, \u003cem\u003eet al\u003c\/em\u003e. Hyriopsis cumingii Hic52-A novel nacreous layer matrix protein with a collagen-like structure[J].Int J Biol Macromol. 2017 Sep;102:667-673.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Gene Bio Systems","offers":[{"title":"250 Units |","offer_id":41524913438820,"sku":"AP231-01","price":342.32,"currency_code":"CAD","in_stock":true},{"title":"500 Units   |","offer_id":41524913471588,"sku":"AP231-02","price":615.69,"currency_code":"CAD","in_stock":true},{"title":"6x500 Units","offer_id":41524913504356,"sku":"AP231-03","price":3142.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/products\/TransStartFastPFUFlybox.png?v=1676739537"},{"product_id":"2x-gb-amp™-pacer™-hp™-master-mix","title":"2x GB-AMP™ PaCeR™ HP™ Master Mix","description":"\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e\u003cstrong\u003e2x GB-AMP™ PaCeR™ HP™ Master Mix\u003c\/strong\u003e is a convenient ready-to-use mixture of GB-AMP™ PaCeR™  HP™ DNA polymerase, dNTP, buffer and magnesium ion. To set up a PCR reaction, you will only need to add the primers, the template and water. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e \u003c\/p\u003e\n\u003ch2 class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e\u003cb\u003e\u003ci\u003eFor a \u003cspan style=\"color: rgb(43, 0, 255);\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_Brochure.pdf?v=1668003992\" style=\"color: rgb(43, 0, 255);\"\u003ebrochure\u003c\/a\u003e \u003c\/span\u003eon this product, please follow this\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_Brochure.pdf?v=1668003992\"\u003e\u003cspan style=\"color: rgb(43, 0, 255);\"\u003e link\u003c\/span\u003e.\u003c\/a\u003e \u003c\/i\u003e\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003eGB-AMP™ PaCeR™  HP™ DNA polymerase was derived from Pfu DNA Polymerase, after several iterative rounds of protein engineering, the enzyme boasts the on-board unique extension factor, specificity-promoting factors and plateau-inhibiting factor. It is a Fast Pfu-derived polymerase. \u003c\/p\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003e\n\u003cstrong\u003eSuper Fidelity:\u003c\/strong\u003e 53-fold higher than Taq DNA Polymerase and 6-fold better than Pfu DNA polymerase\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003e\n\u003cstrong\u003eLong Range capability:\u003c\/strong\u003e amplify fragments as long as 40 kbλ DNA, 20 kb genomic DNA and 10 kb cDNA\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003e\n\u003cstrong\u003eDifficult PCR suitability:\u003c\/strong\u003e Amplify targets with as high as 85% GC\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003e\n\u003cstrong\u003eDirect-PCR: \u003c\/strong\u003eresists inhibitors in crude lysates of bacteria, fungi, whole blood, cultured cells, plant tissues, etc.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003eSuperior Hot-Start enzyme, employing two MAb as molecular thermal switches of polymerase and proof-reading activities.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003e\n\u003cstrong\u003eFast PCR: \u003c\/strong\u003erate of synthesis more than 2x that of Taq DNA polymerase\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003eIn an internal comparison studies, GB-AMP™ PaCeR™  HP™ was compared in the following aspects with the leading all-round enzymes such as Phusion (Thermo) and eight other leading DNA polymerases for PCR:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003eSuccess rate in amplifying human, mouse, rice, wheat, lambda and plasmid DNA, \u003cstrong\u003eranging in size from 305 to 14768 bp\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003eSensitivity, i.e., ability to detect low copy templates,\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003eSpeed of amplification with \u003cstrong\u003eextension time as short as 1 s (for 450 bp) and 5 s (1135 bp)\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003eAmplification of  High GC template with \u003cstrong\u003eGC as high as 85%\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003eGB-AMP™ PaCeR™  HP™ was clearly the best all-round enzyme for robustness, sensitivity, speed, high GC amplification and Direct PCR!\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e\u003ci\u003e\u003cstrong\u003eA tip on the use:\u003c\/strong\u003e \u003c\/i\u003eThe annealing temperature should be chosen based on the Tm values of the primers, in general, 55-65°C. See the user manual for details. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_HP_2x_master_mix_manual-V3_2.pdf?v=1738672872\" target=\"_blank\" title=\"PaCeR Master Mix -User Manual\" rel=\"noopener\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"214\" height=\"60\"\u003e\u003c\/a\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_HP_2x_master_mix_manual-V3_2.pdf?v=1738672872\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cblockquote\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal;\" class=\"MsoNormal\"\u003e\u003cstrong\u003eLooking for a similar DNA Polymerase with higher fidelity and amplification speed? Check out our\u003cspan style=\"color: rgb(43, 0, 255);\"\u003e \u003ca href=\"https:\/\/www.genebiosystems.com\/products\/2-pacer-phanta-flash-master-mix\" style=\"color: rgb(43, 0, 255);\"\u003e2× PaCeR (Phanta) Flash Master Mix (P510)\u003c\/a\u003e\u003c\/span\u003e. \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e\u003ci\u003eThe enzyme generates blunt-ended products and thus products can be cloned by our \u003ca href=\"https:\/\/www.genebiosystems.com\/51-blunt-end-cloning-kits\"\u003eBlunt End Cloning Kits.\u003c\/a\u003e\u003c\/i\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e\u003ci\u003ePlease note: If you are getting a low yield or even no amplification with PaCeR, please consider lowering the annealing temperature by 3-5 °C and\/or increasing the annealing time (doubling). Talk to our Tech Support as you need. \u003c\/i\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCitations\u003c\/strong\u003e\u003c\/p\u003e\n\u003col start=\"1\" type=\"1\"\u003e\n\u003cli data-olk-copy-source=\"MessageBody\" class=\"x_MsoNormal\"\u003eNash D., et al. Hybrid sequencing reveals the genome of a\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eChrysochromulina parva\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003evirus provides insights into nucleocytoplasmic large DNA virus evolution.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eBMC Genomics.\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2025;26:534.\u003cbr\u003edoi:10.1186\/s12864-025-11700-z\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003e\n\u003cspan lang=\"FR\"\u003eDhaliwal J., Sharma P., et al.\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003eProtocol for the efficient and inducible generation of gene knockouts in\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eCandida auris\u003c\/i\u003e.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSTAR Protoc.\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2024;5(3):102639.\u003cbr\u003edoi:10.1016\/j.xpro.2024.102639\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003eDeecker S.R., et al. Type I-F CRISPR-Cas distribution and array dynamics in\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eLegionella pneumophila\u003c\/i\u003e.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eG3 (Bethesda).\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2020;10(3):1039–1050.\u003cbr\u003edoi:10.1534\/g3.119.400908\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003e\n\u003cspan lang=\"FR\"\u003eHurst J.R., et al.\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003eMonkeypox virus shedding despite tecovirimat treatment.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eJ Infect Dis.\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2025; advanced online publication.\u003cbr\u003edoi:10.1093\/infdis\/jiaf471\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003eMonteiro1 VL et al. Ataxin-2 preserves oocyte genome integrity by promoting ribosomal RNA processing.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ebioRxiv.\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003ePosted June 16, 2025.\u003cbr\u003edoi:10.1101\/2025.06.13.659559\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003eRoscow O.M.A. Development of a full-length infectious clone of grapevine fanleaf virus and its application to study host-virus interactions. MSc Thesis. University of Guelph; 2019.\u003cbr\u003eAvailable at:\u003cspan\u003e \u003c\/span\u003e\u003ca data-ogsc=\"\" title=\"https:\/\/atrium.lib.uoguelph.ca\/bitstream\/10214\/17655\/3\/Roscow_Olivia_201912_Msc.pdf?utm_source=chatgpt.com\" data-linkindex=\"0\" href=\"https:\/\/atrium.lib.uoguelph.ca\/bitstream\/10214\/17655\/3\/Roscow_Olivia_201912_Msc.pdf?utm_source=chatgpt.com\" rel=\"noopener noreferrer\" data-auth=\"NotApplicable\" target=\"_blank\"\u003ehttps:\/\/atrium.lib.uoguelph.ca\/bitstream\/10214\/17655\/3\/Roscow_Olivia_201912_Msc.pdf\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003e\n\u003cspan lang=\"FR\"\u003eGoetz J.A., et al.\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003eExploring functional interplay amongst\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eefflux pumps.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eMicrobiology (Reading).\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2022;168(6):001233.\u003cbr\u003edoi:10.1099\/mic.0.001233\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"GeneBio Systems","offers":[{"title":"1mL","offer_id":44444788293732,"sku":"PCR-002-01","price":139.71,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":44444788326500,"sku":"PCR-002-02","price":565.19,"currency_code":"CAD","in_stock":true},{"title":"15mL","offer_id":44444788359268,"sku":"PCR-002-03","price":1270.08,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_better_colors.jpg?v=1743596738"},{"product_id":"gb-amp™-pacer™-hp™-dna-polymerase","title":"GB-AMP™ PaCeR™ HP™ DNA polymerase","description":"\u003cp style=\"margin-bottom: 0cm; line-height: normal;\" class=\"MsoNormal\"\u003e\u003cstrong\u003eGB-AMP™ PaCeR™  HP™ DNA polymerase\u003c\/strong\u003e was derived from Pfu DNA Polymerase, after several iterative rounds of protein engineering, the enzyme boasts the on-board unique extension factor, specificity-promoting factors and plateau-inhibiting factor. It is a Fast Pfu-derived polymerase. \u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0cm; line-height: normal;\" class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch2 class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e\u003cem\u003e\u003cstrong\u003eFor a\u003cspan\u003e \u003c\/span\u003e\u003cspan style=\"color: rgb(43, 0, 255);\"\u003e\u003ca style=\"color: rgb(43, 0, 255);\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_Brochure.pdf?v=1668003992\"\u003ebrochure \u003c\/a\u003e\u003c\/span\u003eon this product, please follow this\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_Brochure.pdf?v=1668003992\"\u003e\u003cspan\u003e \u003c\/span\u003elink.\u003c\/a\u003e \u003c\/strong\u003e\u003c\/em\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli style=\"line-height: normal;\" class=\"MsoNormal\"\u003e\n\u003cstrong\u003eSuper Fidelity:\u003c\/strong\u003e 53-fold higher than Taq DNA Polymerase and 6-fold better than Pfu DNA polymerase\u003c\/li\u003e\n\u003cli style=\"line-height: normal;\" class=\"MsoNormal\"\u003e\n\u003cstrong\u003eLong Range capability:\u003c\/strong\u003e amplify fragments as long as 40 kbλ DNA, 20 kb genomic DNA and 10 kb cDNA\u003c\/li\u003e\n\u003cli style=\"line-height: normal;\" class=\"MsoNormal\"\u003e\n\u003cstrong\u003eDifficult PCR suitability:\u003c\/strong\u003e Amplify targets with as high as 85% GC\u003c\/li\u003e\n\u003cli style=\"line-height: normal;\" class=\"MsoNormal\"\u003e\n\u003cstrong\u003eDirect-PCR: \u003c\/strong\u003eresists inhibitors in crude lysates of bacteria, fungi, whole blood, cultured cells, plant tissues, etc.\u003c\/li\u003e\n\u003cli style=\"line-height: normal;\" class=\"MsoNormal\"\u003eSuperior Hot-Start enzyme, employing two MAb as molecular thermal switches of polymerase and proof-reading activities.\u003c\/li\u003e\n\u003cli style=\"line-height: normal;\" class=\"MsoNormal\"\u003e\n\u003cstrong\u003eFast PCR: \u003c\/strong\u003erate of synthesis more than 2x that of Taq DNA polymerase\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e \u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003eIn an internal comparison studies, GB-AMP™ PaCeR™  HP™ was compared in the following aspects with the leading all-round enzymes such as Phusion (Thermo) and eight other leading DNA polymerases for PCR:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003eSuccess rate in amplifying human, mouse, rice, wheat, lambda and plasmid DNA, \u003cstrong\u003eranging in size from 305 to 14768 bp\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003eSensitivity, i.e., ability to detect low copy templates,\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003eSpeed of amplification with \u003cstrong\u003eextension time as short as 1 s (for 450 bp) and 5 s (1135 bp)\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"line-height: normal;\"\u003eAmplification of  High GC template with \u003cstrong\u003eGC as high as 85%\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eGB-AMP™ PaCeR™ HP™ was clearly the best all-round enzyme for robustness, sensitivity, speed, high GC amplification and Direct PCR!\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003ci\u003e\u003cstrong\u003eA tip on the use:\u003c\/strong\u003e \u003c\/i\u003eThe annealing temperature should be chosen based on the Tm values of the primers, in general, 55-65°C. See the user manual for details. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_HP_Pol_manual-v2_12bbfac3-4041-4436-b8f9-5bf33ccaf59f.pdf?v=1710895436\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"149\" height=\"42\"\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cblockquote\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; line-height: normal;\"\u003e\u003cstrong\u003eLooking for a similar DNA Polymerase with higher fidelity and amplification speed? Check out our\u003cspan style=\"color: rgb(43, 0, 255);\"\u003e \u003ca style=\"color: rgb(43, 0, 255);\" href=\"https:\/\/www.genebiosystems.com\/products\/2-pacer-phanta-flash-master-mix\"\u003e2× PaCeR (Phanta) Flash Master Mix (P510)\u003c\/a\u003e\u003c\/span\u003e. \u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/blockquote\u003e\n\u003cp\u003e\u003cem\u003e\u003cspan\u003eThe enzyme generates blunt-ended products and thus products can be cloned by our \u003ca href=\"https:\/\/www.genebiosystems.com\/51-blunt-end-cloning-kits\"\u003eBlunt End Cloning Kits.\u003c\/a\u003e\u003c\/span\u003e\u003c\/em\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_HP_Pol_manual-v2_12bbfac3-4041-4436-b8f9-5bf33ccaf59f.pdf?v=1710895436\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCitations\u003c\/strong\u003e\u003c\/p\u003e\n\u003col type=\"1\" start=\"1\"\u003e\n\u003cli class=\"x_MsoNormal\" data-olk-copy-source=\"MessageBody\"\u003eNash D., et al. Hybrid sequencing reveals the genome of a\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eChrysochromulina parva\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003evirus provides insights into nucleocytoplasmic large DNA virus evolution.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eBMC Genomics.\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2025;26:534.\u003cbr\u003edoi:10.1186\/s12864-025-11700-z\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003e\n\u003cspan lang=\"FR\"\u003eDhaliwal J., Sharma P., et al.\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003eProtocol for the efficient and inducible generation of gene knockouts in\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eCandida auris\u003c\/i\u003e.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eSTAR Protoc.\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2024;5(3):102639.\u003cbr\u003edoi:10.1016\/j.xpro.2024.102639\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003eDeecker S.R., et al. Type I-F CRISPR-Cas distribution and array dynamics in\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eLegionella pneumophila\u003c\/i\u003e.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eG3 (Bethesda).\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2020;10(3):1039–1050.\u003cbr\u003edoi:10.1534\/g3.119.400908\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003e\n\u003cspan lang=\"FR\"\u003eHurst J.R., et al.\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003eMonkeypox virus shedding despite tecovirimat treatment.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eJ Infect Dis.\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2025; advanced online publication.\u003cbr\u003edoi:10.1093\/infdis\/jiaf471\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003eMonteiro1 VL et al. Ataxin-2 preserves oocyte genome integrity by promoting ribosomal RNA processing.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003ebioRxiv.\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003ePosted June 16, 2025.\u003cbr\u003edoi:10.1101\/2025.06.13.659559\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003eRoscow O.M.A. Development of a full-length infectious clone of grapevine fanleaf virus and its application to study host-virus interactions. MSc Thesis. University of Guelph; 2019.\u003cbr\u003eAvailable at:\u003cspan\u003e \u003c\/span\u003e\u003ca data-auth=\"NotApplicable\" rel=\"noopener noreferrer\" href=\"https:\/\/atrium.lib.uoguelph.ca\/bitstream\/10214\/17655\/3\/Roscow_Olivia_201912_Msc.pdf?utm_source=chatgpt.com\" data-linkindex=\"0\" title=\"https:\/\/atrium.lib.uoguelph.ca\/bitstream\/10214\/17655\/3\/Roscow_Olivia_201912_Msc.pdf?utm_source=chatgpt.com\" data-ogsc=\"\" target=\"_blank\"\u003ehttps:\/\/atrium.lib.uoguelph.ca\/bitstream\/10214\/17655\/3\/Roscow_Olivia_201912_Msc.pdf\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli class=\"x_MsoNormal\"\u003e\n\u003cspan lang=\"FR\"\u003eGoetz J.A., et al.\u003cspan\u003e \u003c\/span\u003e\u003c\/span\u003eExploring functional interplay amongst\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eEscherichia coli\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003eefflux pumps.\u003cspan\u003e \u003c\/span\u003e\u003ci\u003eMicrobiology (Reading).\u003c\/i\u003e\u003cspan\u003e \u003c\/span\u003e2022;168(6):001233.\u003cbr\u003edoi:10.1099\/mic.0.001233\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"GeneBio Systems","offers":[{"title":"100U","offer_id":44445387522148,"sku":"PCR-001-01","price":119.07,"currency_code":"CAD","in_stock":true},{"title":"500U","offer_id":44445387554916,"sku":"PCR-001-02","price":523.91,"currency_code":"CAD","in_stock":true},{"title":"1000U","offer_id":44445387587684,"sku":"PCR-001-03","price":1011.53,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/vials_of_pacer.png?v=1743596738"},{"product_id":"2x-gb-amp™-pacer™-ii-hp™-master-mix","title":"2x GB-AMP™ PaCeR™ II HP™ Master Mix","description":"\u003cp class=\"MsoNormal\" style=\"line-height: 141%; margin: 0cm .05pt 0cm .5pt;\"\u003e\u003cspan style=\"font-size: 10.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003ePaCeR™ II HP™ DNA Polymerase is a superior performance, highly flexible enzyme for robust PCR with extreme fidelity, featuring 53x error rate lower than \u003ci\u003eTaq\u003c\/i\u003e polymerase and extension rate as fast as 1 sec \/ kb*. PaCeR™ II HP™ DNA Polymerase possesses 5´→ 3´polymerase activity, 3´→5´exonuclease activity and will generate blunt-ended products. This product is also capable of amplifying long fragments such as 20 kb genomic DNA and 40 kb λDNA. Our suggested applications include essentially all your PCR applications, including cloning, sequencing, genomic DNA amplification and high throughput PCR, etc. It is ideal for use as all-round, versatile, one-for-all enzyme for a busy and productive PCR lab. It is a convenient 2 x Master Mix format with pre-spiked loading dye. \u003c\/span\u003e\u003c\/p\u003e\n\u003ch1 style=\"margin-left: .5pt;\"\u003e \u003c\/h1\u003e\n\u003ch1 style=\"margin-left: .5pt;\"\u003eAdvantages\u003c\/h1\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify; text-justify: inter-ideograph; line-height: 141%; margin: 0cm .05pt 0cm 3.95pt;\"\u003e\u003cb\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003eRobust: \u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003eHigh probability of PCR success with minimal optimization. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify; text-justify: inter-ideograph; line-height: 141%; margin: 0cm .05pt 0cm 3.95pt;\"\u003e\u003cb\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003eHot Start:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e The enzyme has an inherent hot start capability\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify; text-justify: inter-ideograph; line-height: 141%; margin: 0cm .05pt 0cm 3.95pt;\"\u003e\u003cb\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003eFast:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e up to 12kb\/min\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify; text-justify: inter-ideograph; text-indent: 2.95pt; line-height: 141%; margin: 0cm .05pt 0cm .5pt;\"\u003e\u003cb\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003eExtreme Fidelity:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e 53× error rate lower than that of \u003ci\u003eTaq\u003c\/i\u003e, 6 x lower than that of \u003ci\u003ePfu\u003c\/i\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify; text-justify: inter-ideograph; text-indent: 2.95pt; line-height: 141%; margin: 0cm .05pt 0cm .5pt;\"\u003e\u003cb\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003eHigh Yield:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e Increased product yield compared to those using regular PCR reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify; text-justify: inter-ideograph; text-indent: 2.95pt; line-height: 141%; margin: 0cm .05pt 0cm .5pt;\"\u003e\u003cb\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003eVersatile:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e Recommended for routine PCR, or those with long, and\/or difficult templates or direct PCR\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify; text-justify: inter-ideograph; text-indent: 2.95pt; line-height: 141%; margin: 0cm .05pt 0cm .5pt;\"\u003e\u003cb\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003eExtreme freeze thaw stability:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e at least 15 cycles of freeze thaw tolerated. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify; text-justify: inter-ideograph; text-indent: 2.95pt; line-height: 141%; margin: 0cm .05pt 0cm .5pt;\"\u003e\u003cb\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003eReady to Directly Load to Agarose Gel:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e the master mix contains a gel loading dye that does not impact on PCR amplification\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify; text-justify: inter-ideograph; text-indent: 2.95pt; line-height: 141%; margin: 0cm .05pt 0cm .5pt;\"\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 141%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003ch1 style=\"margin-left: .4pt;\"\u003ePackage Information\u003c\/h1\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm; tab-stops: center 231.6pt 326.9pt 426.9pt;\"\u003e\u003cb style=\"mso-bidi-font-weight: normal;\"\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 112%;\"\u003eComponents\u003cspan style=\"mso-tab-count: 1;\"\u003e                                    \u003c\/span\u003e\u003cspan style=\"mso-spacerun: yes;\"\u003e                       \u003c\/span\u003ePCR-003-01 \u003cspan style=\"mso-tab-count: 1;\"\u003e \u003c\/span\u003e\u003cspan style=\"mso-spacerun: yes;\"\u003e         \u003c\/span\u003ePCR-003-02\u003cspan style=\"mso-spacerun: yes;\"\u003e         \u003c\/span\u003ePCR-003-03\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ctable class=\"TableGrid\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\" width=\"647\" style=\"width: 485.55pt; margin-left: .45pt; border-collapse: collapse; mso-yfti-tbllook: 1184; mso-padding-alt: 3.4pt 0cm 3.2pt 0cm;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"mso-yfti-irow: 0; mso-yfti-firstrow: yes; mso-yfti-lastrow: yes; height: 18.15pt;\"\u003e\n\u003ctd width=\"269\" valign=\"top\" style=\"width: 201.95pt; border-top: solid #221714 1.0pt; border-left: none; border-bottom: solid #221714 1.0pt; border-right: none; mso-border-top-alt: solid #221714 .75pt; mso-border-bottom-alt: solid #221714 .75pt; padding: 3.4pt 0cm 3.2pt 0cm; height: 18.15pt;\"\u003e\n\u003cp class=\"MsoNormal\" style=\"margin: 0cm 0cm 0cm .75pt;\"\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 112%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e2× PaCeR™ II HP™ Master Mix\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"30\" valign=\"top\" style=\"width: 22.6pt; border-top: solid #221714 1.0pt; border-left: none; border-bottom: solid #221714 1.0pt; border-right: none; mso-border-top-alt: solid #221714 .75pt; mso-border-bottom-alt: solid #221714 .75pt; padding: 3.4pt 0cm 3.2pt 0cm; height: 18.15pt;\"\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm;\"\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 112%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"157\" valign=\"top\" style=\"width: 117.65pt; border-top: solid #221714 1.0pt; border-left: none; border-bottom: solid #221714 1.0pt; border-right: none; mso-border-top-alt: solid #221714 .75pt; mso-border-bottom-alt: solid #221714 .75pt; padding: 3.4pt 0cm 3.2pt 0cm; height: 18.15pt;\"\u003e\n\u003cp class=\"MsoNormal\" style=\"margin-bottom: 0cm;\"\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 112%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e\u003cspan style=\"mso-spacerun: yes;\"\u003e         \u003c\/span\u003e1 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"107\" valign=\"top\" style=\"width: 80.35pt; border-top: solid #221714 1.0pt; border-left: none; border-bottom: solid #221714 1.0pt; border-right: none; mso-border-top-alt: solid #221714 .75pt; mso-border-bottom-alt: solid #221714 .75pt; padding: 3.4pt 0cm 3.2pt 0cm; height: 18.15pt;\"\u003e\n\u003cp class=\"MsoNormal\" style=\"margin: 0cm; text-indent: 0cm;\"\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 112%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e5 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"84\" valign=\"top\" style=\"width: 63.0pt; border-top: solid #221714 1.0pt; border-left: none; border-bottom: solid #221714 1.0pt; border-right: none; mso-border-top-alt: solid #221714 .75pt; mso-border-bottom-alt: solid #221714 .75pt; padding: 3.4pt 0cm 3.2pt 0cm; height: 18.15pt;\"\u003e\n\u003cp class=\"MsoNormal\" style=\"margin: 0cm; text-indent: 0cm;\"\u003e\u003cspan style=\"font-size: 10.0pt; mso-bidi-font-size: 11.0pt; line-height: 112%; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Arial Unicode MS';\"\u003e15 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeR_II_HP_2x_Master_Mix_Manual.pdf?v=1777168871\" target=\"_blank\" title=\"PaCeR II Master Mix -User Manual\" rel=\"noopener\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/usermanualbutton_2d5025b5-57b4-4ac5-8bf3-ee7038ddf8fa_480x480.png?v=1700366910\" width=\"214\" height=\"60\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"GeneBio Systems","offers":[{"title":"1mL","offer_id":48316069544036,"sku":"PCR-003-01","price":153.68,"currency_code":"CAD","in_stock":true},{"title":"5mL","offer_id":48316069576804,"sku":"PCR-003-02","price":621.7,"currency_code":"CAD","in_stock":true},{"title":"15mL","offer_id":48316069609572,"sku":"PCR-003-03","price":1397.09,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0558\/8588\/9636\/files\/PaCeRIIimage.png?v=1777168677"}],"url":"https:\/\/www.genebiosystems.com\/en-de\/collections\/long-pcr.oembed","provider":"GeneBio ","version":"1.0","type":"link"}