Species Reactivity:Rat (Rattus norvegicus)
UniProt:P27435
Abbreviation:TPSAB1
Alternative Names:TPS1; TPS2; TPSB1; mast cell alpha II tryptase|mast cell beta I tryptase|tryptase alpha II|tryptase beta 1|tryptase beta I|tryptase-I|tryptase-III
Application:ELISA
Range:0.156-10 ng/mL
Sensitivity:0.055 ng/mL
Intra-AssayCV:?5.2%
Inter-AssayCV:?7.7%
Recovery:1
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Quantitive
Test principle:This assay employs a two-site sandwich ELISA to quantitate TPSAB1 in samples. An antibody specific for TPSAB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPSAB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPSAB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPSAB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. TPSb2 is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).