T7 High Efficiency Transcription Kit

JT101

T7 High Efficiency Transcription Kit is designed for in vitro RNA synthesis by T7 RNA Polymerase with supercoiled or linearized DNA templates. Up to 150 μg of RNA can be produced from a 20 μl reaction. Synthesized RNA can be used for in vitro translation, RNase protection assays, RNA splicing, and hybridization assays.

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CAD$270.00

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Description

T7 High Efficiency Transcription Kit is designed for in vitro RNA synthesis by T7 RNA Polymerase with supercoiled or linearized DNA templates. Up to 150 μg of RNA can be produced from a 20 μl reaction. Synthesized RNA can be used for in vitro translation, RNase protection assays, RNA splicing, and hybridization assays.

Storage

at -20°C for one year

Kit Content

Component

JT101-01 (25 rxns)

T7 Transcription Enzyme Mix

50 µl

5×T7 Transcription Reaction Buffer

100 µl

10 mM NTP Mix

200 µl

DNase I (1 unit/µl)

25 µl

500 mM EDTA (pH 8.0)

25 µl

RNase-free Water

500 µl

Control Transcription Template (0.5 µg/µl)

10 µl

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