pEASY®-Uni Seamless Cloning and Assembly Kit

This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.

•Fast: 15 min

•Broad: no restriction enzyme digestions. Can be cloned into any sites

•Up to 95% cloning efficiency

•Seamless: no extra sequences introduced; up to 5 fragments assembly

More details

CAD$215.00

  • 10 reactions
  • 20 reactions
  • 60 reactions

More info

Description

This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.

•Fast: 15 minutes.

•Broad: no restriction enzyme digestions. Can be cloned into any sites.

•High efficiency: up to 95% cloning efficiency.

•Seamless: no extra sequences introduced; up to 5 fragments assembly.

Storage

Competent Cell at -70°C for six months; others at -20°C for 9 months

Kit Content

Component

CU101-01

CU101-02

CU101-03

2×Assembly Mix

50 μl

100 μl

3×100 μl

Linearized pUC19 Control Vector (10 ng/μl)

3 μl

6 μl

18 μl

Control Insert (1 kb,20 ng/μl)

3 μl

6 μl

18 μl

Trans1-T1 Phage Resistant Chemically Competent Cell

5×100 μl

10×100 μl

30×100 μl

References:

LiteratureJournalIFAuthorDateLink
Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathwayMETABOLIC ENGINEERING8.142Wang Y, et al. State Key Laboratory of Microbial M2017 JanThe original link
A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging systemnew phytologist7.21Borong Lin, et al.2015 OctThe original link
Phosphorylation-mediated Regulatory Networks in Mycelia of Pyricularia oryzae Revealed by Phosphoproteomic AnalysesMOLECULAR & CELLULAR PROTEOMICS6.5399Ruijin Wang,et al.2017JulThe original link
The maize transcription factor EREB58 mediates the jasmonate-induced production of sesquiterpene volatiles.Plant journal5.4679Li S, et al. Biotechnology Research Institute, Chi2015 OctThe original link
Characterization of Pseudooxynicotine Amine Oxidase of Pseudomonas putida S16 that Is Crucial for Nicotine Degradation.Scientific Reports5.2278Hu H, et al. State Key Laboratory of Microbial Met2015 DecThe original link

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