pEASY®-T1 Cloning Kit

pEASY ®-T1 Cloning Kit is designed for cloning Taq-amplified PCR products.

•5 minutes fast ligation of PCR products.

•Choice of Kanamycin or Ampicillin resistance genes.

•Blue/white selection.

•T7 promoter, M13 forward and M13 reverse primers for sequencing.

•Includes Trans1-T1 Phage Resistant Chemical Competent Cells, high efficiency (>10^9 cfu/μg pUC19 DNA) and fast growth rate.

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CAD$296.00

  • 20 reactions
  • 60 reactions

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Description

pEASY ®-T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products.

•5 minutes fast ligation of Taq-amplified PCR products.

•Kanamycin and Ampicillin resistance genes for selection.

•Easy blue/white selection.

•T7 promoter, M13 forward and M13 reverse primers for sequencing.

•T7 promoter for in vitro transcription.

  • Trans1-T1 Phage Resistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70°C for six months; others at -20°C for 9 months

Kit Content

Component

CT101-01

CT101-02

(20 rxns)

(60 rxns)

pEASY ®-T1 Cloning Vector (10 ng/μl)

20µl

3×20 µl

Control Template (5 ng/μl)

5µl

5µl

Control Primers (10 μM)

5µl

5µl

M13 Forward Primer (10 μM)

50µl

150µl

M13 Reverse Primer (10 μM)

50µl

150µl

Trans1-T1 Phage Resistant

10×100 μl

30×100 μl

Chemically Competent Cells

References:

LiteratureJournalIFAuthorDateLink
A maize wall-associated kinase confers quantitative resistance to head smutNature Genetics29.648Weiliang Zuo et al.2014 DecThe original link
An Adaptive Transposable Element Insertion in the Regulatory Region of the EO Gene in the Domesticated Silkworm, Bombyx moriMolecular Biology and Evolution14.308Wei Sun, et al.2014 OctThe original link
pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic ElementsLife9.322Xiaoyu Xiang, et al.2015 FebThe original link
Undifferentiated embryonic cell transcription factor-1 (UTF1) inhibits the growth of cervical cancer cells by transactivating p27 Kip1Carcinogenesis5.635Wu X L, Zheng P S. Department of Reproductive Medi2013 JulThe original link
Differential regulation of taurine biosynthesis in rainbow trout and Japanese flounderSci Rep.5.578Xuan Wang, et al.2016 FebThe original link

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