Trans5α Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^8 cfu/μg DNA (tested by pUC19 plasmid DNA).
High efficiency: >10^8 cfu/μg ( pUC19 DNA)./Reduced recombination of cloned DNA./Blue/white selection.
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Trans5α Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA).
F-φ80d lacZΔM15 Δ(lacZYA-argF) U169 end A1 recA1 hsdR17 (rk-, mk+) supE44λ- thi-1 gyrA96relA1 phoA
• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
• Reduced recombination of cloned DNA.
• Blue/white selection.
Competent Cell at -70°C for six months; others at -20°C for one year
Higher efficiency transformation can be achieved by transforming cells immediately following thawing.
Avoid repeated thawing.
Gentle handling is required for the entire procedure.
|Fungicides induced triazole-resistance in Aspergillus fumigatus associated with mutations of TR46/Y121F/T289A and its appearance in agricultural fields||Journal of Hazardous Materials||6.065||Jingbei Ren,et al.||2017Mar||The original link|
|Automatic Enumeration of Gold Nanomaterials at the Single-Particle Level||Anal Chem||5.886||Xiao Xu, et al.||2015 Feb||The original link|
|Utilization of biodiesel by-product as substrate for high-production of ??-farnesene via relatively balanced mevalonate pathway in Escherichia coli||Bioresource Technology||5.65||Shengping You et al||2017 Jun||The original link|
|The antitumor activity and preliminary modeling on the potential mechanism of action of human peroxiredoxin-5||Oncotarget||5.1657||Juanjuan Liu,et al.||2017Mar||The original link|
|Monitoring and evaluation of antibiotic resistance genes in four municipal wastewater treatment plants in Harbin, Northeast China||Environ Pollut||5.0989||Wen Q, et al.||2016 Jan||The original link|