pEASY®-Blunt Zero Cloning Kit

pEASY ®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white selection is not required.

•5 minutes fast ligation

•High cloning efficiency. Positive clones up to 100%

•No blue/white selection needed

More details

CAD$377.00

  • 20 reactions
  • 60 reactions

More info

Description

pEASY ®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white selection is not required.

•5 minutes fast ligation of Pfu-amplified PCR products.

•High cloning efficiency. Positive clones up to 100%.

•No blue/white selection needed.

•Suitable for larger fragment cloning.

•Kanamycin and Ampicillin resistance genes for selection.

•M13 forward primer and M13 reverse primer for sequencing.

•T3 promoter and T7 promoter for in vitro transcription.

  • Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70°C for six months; others at -20°C for 9 months

Kit Content

Component

CB501-01

CB501-02

(20 rxns)

(60 rxns)

pEASY ®-Blunt Zero Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)

5 µl

5 µl

Control Primers (10 μM)

5 µl

5 µl

M13 Forward Primer (10 μM)

50 µl

150 µl

M13 Reverse Primer (10 μM)

50 µl

150 µl

Trans 1-T1 Phage Resistant

10×100 μl

30×100 μl

Chemically Competent Cells

References:

LiteratureJournalIFAuthorDateLink
NdhV is a subunit of NADPH dehydrogenase essential for cyclic electron transport in Synechocystis sp. strain PCC 6803plant physiology6.28Gao F, et al. College of Life and Environment Scie2016 FebThe original link
Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB.Scientific Reports5.228He W, et al. Key Laboratory of Combinatorial Biosy2015 JulThe original link
Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stressFRONTIERS IN PLANT SCIENCE4.495Wang H, et al. Key Laboratory of Coastal Biology a2015 SepThe original link
CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacumplant molecular biology3.905Gao J, et al. State Key Laboratory of Silkworm Gen2015 JanThe original link
The Different Potential of Sponge Bacterial Symbionts in N2 Release Indicated by the Phylogenetic Diversity and Abundance Analyses of Denitrification Genes, nirK and nosZPLoS One3.73Zhang X, et al. Marine Biotechnology Laboratory, S2013 JunThe original link

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