DMT Chemical Competent cells have high transformation efficiency: >10ˆ8 cfu/μg (pUC19 DNA), are resistant to T1 and T5 phage. The cells harbor DMT enzyme (DpnI) to achieve in vivo digestion of methylated DN and are ideal for some site-directed mutagenesis protocols.
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F- φ80 lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 thi-1 gyrA96relA1 tonA
• High transformation efficiency: >108 cfu/μg (pUC19 DNA).
• Resistance to T1 and T5 phage.
• In vivo digestion of methylated DNA, suitable for site-directed mutagenesis.
at -70 °C for six months
Higher efficiency transformation can be achieved by transforming cells immediately following thawing.
Avoid repeated thawing.
Gentle handling is required for the entire procedure.
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