DMT Chemically Competent Cell

DMT Chemical Competent cells have high transformation efficiency: >10ˆ8 cfu/μg (pUC19 DNA), are resistant to T1 and T5 phage. The cells harbor DMT enzyme (DpnI) to achieve in vivo digestion of methylated DN and are ideal for some site-directed mutagenesis protocols.

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CAD$123.00

  • 20 reactions
  • 40 reactions

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  • DMT Chemical Competent cells have high transformation efficiency: >108 cfu/μg (pUC19 DNA), are resistant to T1 and T5 phage. The cells harbor DMT enzyme (DpnI) to achieve in vivo digestion of methylated DN and are ideal for some site-directed mutagenesis protocols.

Genotype

F- φ80 lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 thi-1 gyrA96relA1 tonA

Features

• High transformation efficiency: >108 cfu/μg (pUC19 DNA).

• Resistance to T1 and T5 phage.

• In vivo digestion of methylated DNA, suitable for site-directed mutagenesis.

Storage

at -70 °C for six months

Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.

References:

LiteratureJournalIFAuthorDateLink
Characterization of EndoTT, a novel single-stranded DNA-specific endonuclease from Thermoanaerobacter tengcongensisnucleic acids research9.202Zhang S, et al. State Key Laboratory of Microbial2010 JunThe original link
Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysisBiotechnology for Biofuels6.4439Nanyu Han,et al.2017 MayThe original link
A Novel Enterovirus 71 (EV71) Virulence Determinant: The 69th Residue of 3C Protease Modulates PathogenicityFront Cell Infect Microbiol5.2179Bingqing Li,et al.2017FebThe original link
The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/??-Glucanase That Can Degrade Crystalline CelluloseApplied and Environmental Microbiology3.668Xianli Xue, et al.2015 MarThe original link

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