Trans1-Blue Chemically Competent Cell

Trans1-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^8 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR), featuring:

/High transformation efficiency: >10^8 cfu/μg ( pUC19 DNA)/TetR /Blue/white selection.

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CAD$177.00

  • 20 reactions
  • 40 reactions

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Description

Trans1-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR).

Genotype

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 (rk-,mk+), relA1 lac [F' proAB lacIqZΔM15: Tn10 (TetR)]

Features

• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).

• TetR.

• Blue/white selection.

Storage

at -70 °C for six months

Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.

References:

LiteratureJournalIFAuthorDateLink
The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/??-Glucanase That Can Degrade Crystalline CelluloseApplied and Environmental Microbiology3.668Xianli Xue, et al.2015 MarThe original link
Controlling microbial PHB synthesis via CRISPRiapplied microbiology and biotechnology3.42Dan Li,et al.2017MayThe original link
Molecular cloning and characterization of DXS and DXR genes in the terpenoid biosynthetic pathway of Tripterygium wilfordiiinternational journal of molecular sciences3.257Tong Y, et al. School of Traditional Chinese Medic2015 OctThe original link
Metabolic engineering of an E. coli ndh knockout strain for PHB production from mixed glucose-xylose feedstockjournal of chemical technology and biotechnology3.1349Guangxin Huo,et al.2017MayThe original link
Engineering Escherichia coli for poly-(3-hydroxybutyrate) production guided by genome-scale metabolic network analysisenzyme and microbial technology2.502Yangyang Zheng,et al.2017NovThe original link

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