TransDB3.1 Chemically Competent Cell

Description

TransDB3.1 Chemically Competent Cell is specifically designed for chemical transformation of DNA. This cell contains the gyrA462 gene which provides resistance to the toxic effects from the ccdB gene. TransDB3.1 Chemically Competent Cell can be used for transformation and propagation of plasmid containing the ccdB gene. It permits a transformation efficiency of over 10⁸ cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype

F- gyrA462 endA1 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) supE44ara-14 galK2 lacY1 proA2rpsL20(SmR) xyl-5 λ- leu mtl1

Features

• High transformation efficiency: >108 cfu/μg (pUC19 DNA).

• Transformation and propagation of plasmids containing the ccdB gene.

• StrR.

Storage

at -70 °C for six months

Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.