Trans10 Chemically Competent Cell

Trans10 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^8 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to streptomycin sulfate (StrR), featuring:

High efficiency: >10^8 cfu/μg ( pUC19 DNA)./StrR./ Blue/white selection/Toxic gene cloning; stable replication of plasmid DNA.

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CAD$144.00

  • 20 reactions
  • 40 reactions

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Description

Trans10 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to streptomycin sulfate (StrR).

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA1 araΔ139Δ(ara-leu)7697 galU galKrpsL (StrR) endA1 nupG

Features

• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).

• StrR.

• Blue/white selection.

• Toxic gene cloning and stable replication of plasmid DNA.

Storage

at -70 °C for six months

Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.

References:

LiteratureJournalIFAuthorDateLink
MAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback mothPLoS Genetics6.6609Zhaojiang Guo2015 AprThe original link
Improved flux and anti-biofouling performances of reverse osmosis membrane via surface layer-bylayer assemblyjournal of membrane science6.0349Yao Wang,et al.2017OctThe original link
Producing aglycons of ginsenosides in bakers' yeastScientific Reports5.5779Zhubo Dai, et al. Tianjin Institute of Industrial2014 JanThe original link
Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegansScientific Reports5.5779Chen X, et al. School of Life Sciences, University2014 DecThe original link
Heterologous Expression of Helicoverpa armigera Cytochrome P450 CYP6B7 in Pichia pastoris and Interactions of CYP6B7 with Insecticidespest management science3.253Chunqing Zhao,et al.2017MarThe original link

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