Trans10 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^8 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to streptomycin sulfate (StrR), featuring:
High efficiency: >10^8 cfu/μg ( pUC19 DNA)./StrR./ Blue/white selection/Toxic gene cloning; stable replication of plasmid DNA.
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Trans10 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to streptomycin sulfate (StrR).
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA1 araΔ139Δ(ara-leu)7697 galU galKrpsL (StrR) endA1 nupG
• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
• Blue/white selection.
• Toxic gene cloning and stable replication of plasmid DNA.
at -70 °C for six months
Higher efficiency transformation can be achieved by transforming cells immediately following thawing.
Avoid repeated thawing.
Gentle handling is required for the entire procedure.
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