pEASY®-Blunt3 Cloning Kit

pEASY ®- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning Pfu-amplified PCR products.

•5 min fast ligation

•Ampicillin resistance marker

•Blue/white selection

•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.

•Includes Competent Cell, high efficiency (>10^9 cfu/μg pUC19 DNA) and fast growing.

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CAD$296.00

  • 20 reactions
  • 60 reactions

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Description

pEASY ®- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion.

•5 minutes fast ligation of Pfu-amplified PCR products.

•Ampicillin resistance gene for selection.

•Easy blue/white selection.

•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.

•T7 promoter and SP6 promoter for in vitro transcription.

  • Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

Storage

Competent Cell at -70℃ for six months; others at -20℃ for 9 months

Kit Content

Component

CB301-01

CB301-02

(20 rxns)

(60 rxns)

pEASY ®-Blunt3 Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)

5 µl

5 µl

Control Primers (10 μM)

5 µl

5 µl

M13 Forward Primer (10 μM)

50 µl

150 µl

M13 Reverse Primer (10 μM)

50 µl

150 µl

Trans1-T1 Phage Resistant

10×100 μl

30×100 μl

Chemically Competent Cell

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