Trans1-T1 Phage Chemically Competent Cell

Trans1-T1 Phage Resistant Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10^9 cfu/μg DNA (tested by pUC19 plasmid DNA).

• High efficiency: >10^9 cfu/μg.

• Fast-growth.

• Resistance to T1 and T5 phage.

• Blue/white selection.

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CAD$99.00

  • 10 reactions
  • 20 reactions
  • 40 reactions

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Description

Trans1-T1 Phage Resistant Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 109 cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype

F-φ80(lacZ)ΔM15ΔlacX74hsdR(rk-, mk+)ΔrecA1398endA1tonA

Features

• High transformation efficiency: >109 cfu/μg (pUC19 DNA).

• Fast-growing, colonies are visible in 8~9 hours.

• Resistance to T1 and T5 phage.

• Blue/white selection.

Storage

at -70 °C for six months

Notes

Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.

References:

LiteratureJournalIFAuthorDateLink
Removal of antibiotic resistance genes from wastewater treatment plant effluent by coagulationwater research6.9419Na Li,et al.2017MarThe original link
Production and characterization of poly(3-hydroxypropionate-co-4-hydroxybutyrate) with fully controllable structures by recombinant Escherichia coli containing an engineered pathwayMetabolic Engineering6.859Meng DC, et al. MOE Key Lab of Bioinformatics, Dep2012 JulThe original link
Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplificationanalytical chemistry6.32Yi Hu,et al.2016NovThe original link
Prevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 yearsjournal of hazardous materials6.065Shuang Peng,et al.2017QctThe original link
Effect of fumigation with chloropicrin on soil bacterial communities and genes encoding key enzymes involved in nitrogen cyclingEnvironmental Pollution5.0989Jun Li,et al.2017AugThe original link

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