Trans109 Chemically Competent Cell


Trans109 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA).

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  • 20 reactions
  • 40 reactions

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endA1 recA1 gyrA96 thi-1 hsdR17 (rk-, mk+relA1 supE44 D (lac-proAB) [F′traD36 proAB laqIqZΔM15]


• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).

• The lowest homologous recombination favorable for plasmid DNA preparation.

• Routine cloning.

• Blue/white selection.


at -70 °C for six months


Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

Avoid repeated thawing.

Gentle handling is required for the entire procedure.


MAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback mothPLoS Genetics8.167Zhaojiang Guo?? et al2015 AprThe original link
Bacillus thuringiensis subsp. sichuansis strain MC28 produces a novel crystal protein with activity against Culex quinquefasciatus larvaeWorld Journal of Microbiology and Biotechnology1.262Peng Guan, et al. State Key Laboratory of Hybrid R2014 AprThe original link
Cloning and characterisation of a novel cry1H gene effective against lepidopterous larvae from a Bacillus thuringiensis strainBiocontrol Science and Technology0.848Yu Z, et al. Sichuan agricultural university2015The original link

LiteratureJournalIFAuthorDateLinkDown-regulation of a novel ABC transporter gene (Pxwhite) is associated with Cry1Ac resistance in the diamondback moth, Plutella xylostella (L.)Insect Biochemistry and Molecular Biology3.42Zhaojiang Guo?? et al2015 JanThe original linkCloning, expression, and characterization of a novel xylose reductase from Rhizopus oryzaeJournal of Basic Microbiology1.823M Zhang, et al.2015 FebThe original linkProduction of poly(3-hydroxypropionate) and poly(3-hydroxybutyrate-co-3-hydroxypropionate) from glucose by engineering Escherichia coli0De-Chuan Meng, et al.2015 AprThe original link

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