Size: 10 reactions
pEASY®-Blunt M3 Expression Vector utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector under regulation of enhanced CMV promoter. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.
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• 5 minutes fast ligation of Pfu-amplified PCR products.
• Enhanced CMV promoter for higher protein yield.
• CMV forward primer and TK polyA reverse primer for sequencing.
• C-terminal Flag-tag for protein detection.
• Neomycin resistance gene for stable cell line selection.
• M3 Expression Plasmid included as negative control.
Competent Cell at -70°C for six months; others at -20°C for nine months
CM311-01 (10 rxns)
pEASY ®-Blunt M3 Expression Vector (15 ng/μl)
M3 Expression Plasmid (Negative Control) (15 ng/μl)
MControl Template (5 ng/μl)
MControl Forward Primers (10 μM)
MControl Reverse Primers (10 μM)
CMV Forward Primer (10 μM)
TK PolyA Reverse Primer (10 μM)
Trans1-T1 Phage Resistant
Chemically Competent Cell